Key Product Details

Species Reactivity

Validated:

Multi-Species

Cited:

Human

Applications

Validated:

Intracellular Staining by Flow Cytometry

Cited:

Flow Cytometry

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # AD1.1.10
View all His Tag Primary Antibodies »

Product Specifications

Immunogen

His-tagged peptide

Specificity

Detects proteins containing accessible consecutive histidine regions. The antibody detects His tags localized at the amino- or carboxyl-terminus.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for His Tag Alexa Fluor® 488‑conjugated Antibody

Detection of His Tag in HEK293 cell line transfected with His-tagged protein cells by Flow Cytometry.

HEK293 cell line transfected with His-tagged protein cells were stained with Mouse Anti-His Tag Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC050G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with Flow Cytometry Fixation Buffer (Catalog # FC004). View our protocol for Staining Intracellular Molecules.

Applications for His Tag Alexa Fluor® 488‑conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

0.5 µg/106 cells
Sample: HEK293 cell line transfected with His-tagged protein

Reviewed Applications

Read 3 reviews rated 5 using IC050G in the following applications:

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: His Tag

Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected or purified by using anti-polyHis antibodies.

Long Name

Histidine Tag

Alternate Names

polyHistidine

Additional His Tag Products

Product Documents for His Tag Alexa Fluor® 488‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for His Tag Alexa Fluor® 488‑conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

Citations for His Tag Alexa Fluor® 488‑conjugated Antibody

Customer Reviews for His Tag Alexa Fluor® 488‑conjugated Antibody (3)

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Showing  1 - 3 of 3 reviews Showing All
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  • His Tag Alexa Fluor® 488-conjugated Antibody
    Name: Jenna Nguyen
    Application: Affinity Purification
    Sample Tested: 4T1 mouse breast cancer cell line
    Species: Mouse
    Verified Customer | Posted 02/08/2019
  • His Tag Alexa Fluor® 488-conjugated Antibody
    Name: Jenna Nguyen
    Application: ELISA
    Sample Tested: 3T3-L1 mouse embryonic fibroblast adipose-like cell line
    Species: Mouse
    Verified Customer | Posted 01/30/2019
  • His Tag Alexa Fluor® 488-conjugated Antibody
    Name: Jenna Nguyen
    Application: Cell depletion
    Sample Tested: 293T human embryonic kidney cell line
    Species: Human
    Verified Customer | Posted 12/06/2018

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