His Tag Alexa Fluor® 488-conjugated Antibody Summary
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of His tag in HEK293 Human Cell Line Transfected with His-tagged Protein by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with His-tagged protein was stained with Mouse Anti-His Tag Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC050G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I
(Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: His Tag
Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected or purified by using anti-polyHis antibodies.
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Reviews for His Tag Alexa Fluor® 488-conjugated Antibody
There are currently no reviews for this product. Be the first to review His Tag Alexa Fluor® 488-conjugated Antibody and earn rewards!
Have you used His Tag Alexa Fluor® 488-conjugated Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image