Intracellular Staining by Flow Cytometry
Detection of His Tag in NS0 Mouse Cell Line Transfected with His-tagged Protein by Flow Cytometry. NS0 mouse myeloma cell line transfected with His-tagged protein was stained with Mouse Anti-His Tag PerCP‑conjugated Monoclonal Antibody (Catalog # IC050C, filled histogram) or isotype control antibody (Catalog # IC002C, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I|
(Catalog # FC005). View our protocol for Staining Intracellular Molecules.
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