Histone H3 [Citrulline Arg17, Citrulline Arg2, Citrulline Arg8] Antibody

Novus Biologicals | Catalog # NB100-57135

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Bacteria

Cited:

Human, Mouse, Rat, Bacteria, WB

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation (ChIP)

Cited:

Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Histone H3 Primary Antibodies »

Product Specifications

Immunogen

This Histone H3 antibody was raised against synthetic peptide derived from N terminal region of Human Histone H3, containing Citrulline at position 2;8;17

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 31160588). Use in Rat reported in scientific literature (PMID:32890741). Bacteria reactivity reported in (PMID: 32329205).

Modification

Citrulline Arg17, Citrulline Arg2, Citrulline Arg8

Specificity

Reacts with human Histone H3 Citrulline at the 3 positions: 2, 8 and 17. Reacts with mono or di H3-Cit. Not tested in other species. May cross reacts with other species due to sequence homology.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images

Immunocytochemistry/ Immunofluorescence: Histone H3 [Citrulline Arg17, Citrulline Arg2, Citrulline Arg8] Antibody [NB100-57135]

Immunocytochemistry/ Immunofluorescence: Histone H3 [Citrulline Arg17, Citrulline Arg2, Citrulline Arg8] Antibody [NB100-57135]

Immunocytochemistry/Immunofluorescence: Histone H3 [Citrulline 2, Citrulline 8, Citrulline 17] Antibody [NB100-57135] - HL60 cells treated with DMSO and Calcimycin Green: (at 1:1000 Blue Hoechst)
Western Blot: Histone H3 [Citrulline Arg17, Citrulline Arg2, Citrulline Arg8] Antibody [NB100-57135]

Western Blot: Histone H3 [Citrulline Arg17, Citrulline Arg2, Citrulline Arg8] Antibody [NB100-57135]

Western Blot: Histone H3 [Citrulline 2, Citrulline 8, Citrulline 17] Antibody [NB100-57135] - Histone H3/Citrulline (R2,R8,R17) at 1:10000 dilution. Observed molecular weight is ~15 kDa.
Histone H3 [Citrulline Arg17, Citrulline Arg2, Citrulline Arg8] Antibody

Immunohistochemistry: Rabbit Polyclonal Histone H3 [Citrulline Arg17, Citrulline Arg2, Citrulline Arg8] Antibody [NB100-57135]

Immunohistochemistry: Rabbit Polyclonal Histone H3 [Citrulline Arg17, Citrulline Arg2, Citrulline Arg8] Antibody [NB100-57135] - Analysis of Histone H3 on mouse brain tissue. Nucleus (Blue, DAPI) ; H3cit (green). Hemorrhagic stroke model FxFr ; 10 µm thickness 1:400 Secondary antibody AF488. Image from a verified customer review.
Histone H3 [Citrulline Arg17, Citrulline Arg2, Citrulline Arg8] Antibody

Immunocytochemistry/ Immunofluorescence: Histone H3 [Citrulline Arg17, Citrulline Arg2, Citrulline Arg8] Antibody [NB100-57135] -

CLEC2.Fc inhibits SARS‐CoV‐2‐induced immunothrombosis in lungAAAV‐hACE2 mice were treated with saline and challenged with SARS‐CoV‐2 (8 × 104 PFU) for 5 days. Lung tissue sections were stained with DAPI (blue), anti‐MPO antibody (green), anti‐citrullinated histone H3 (Cit‐H3) antibody (red), anti‐CD42b antibody (yellow) and anti‐CD31 antibody (gray). yellow arrow: NETs (DNA+Gr‐1+MPO+Cit‐H3+); red arrow: immunothrombosis (NETs + thrombus (CD42b+)), Scale bar is 100 μm.B, CFor prophylactic treatment, CLEC2.Fc (or vehicle) was given at 1 h before virus challenge; for therapeutic treatment, CLEC2.Fc was given at 8 h post‐infection. The area of NET (colocalization area of MPO and Cit‐H3) (B) and immunothrombosis (colocalization area of Cit‐H3 and CD42b) (C) were measured using MetaMorph software.Data information: Data are mean +/- SEM from two independent experiments (saline: n = 3 for 3 d.p.i., n = 4 for 5 d.p.i.; isotype: n = 3 for 3 d.p.i., n = 4 for 5 d.p.i.; prophylactic treatment of CLEC2.Fc treatment: n = 5 for 3 d.p.i. and 5 d.p.i.; therapeutic treatment of CLEC2.Fc: n = 3 for 3 d.p.i. and 5 d.p.i.). *P < 0.05, **P < 0.01 (two‐way ANOVA).Source data are available online for this figure. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37211986), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:10-1:500

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:5000 - 1:50000
Application Notes
Use in IHC-Frozen reported in scientific literature (PMID:32890741).

Reviewed Applications

Read 3 reviews rated 4 using NB100-57135 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Unpurified

Reconstitution

Lyophilized powder, reconstitute with 0.2 ml distilled water.

Formulation

Antiserum

Preservative

No Preservative

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Histone H3

Chromatin is an arrangement of DNA and proteins which form chromosomes. Chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is very dynamic, where post-translational modifications can regulate DNA to be copied, transcribed, or repaired.

Histones are nuclear proteins responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Changes in chromatin structure play a large role in the regulation of transcription. The chromatin fibers are compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.

Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. Posttranslational modifications such as acetylation of core histones regulates gene expression, thus altering protein function and regulation (1). Histone H3 is primarily acetylated at lysines 9, 14, 18, and 23 and have a theoretical molecular weight of 15 kDa. Acetylation at lysine 9 and 14 appears to control histone deposition, chromatin assembly and active transcription. Methylation of arginine residues within histone H3 has also been linked to transcription regulation. Histone H3 has been linked to various types of cancer as a biomarker through the aberrant expression of histone deacetylase (HDAC) enzymes and changes to chromatins (2-4).

References

1. Zhang, Y. X., Akumuo, R. C., Espana, R. A., Yan, C. X., Gao, W. J., & Li, Y. C. (2018). The histone demethylase KDM6B in the medial prefrontal cortex epigenetically regulates cocaine reward memory. Neuropharmacology, 141, 113-125. doi:10.1016/j.neuropharm.2018.08.030

2. Nandakumar, V., Hansen, N., Glenn, H. L., Han, J. H., Helland, S., Hernandez, K,...Meldrum, D. R. (2016). Vorinostat differentially alters 3D nuclear structure of cancer and non-cancerous esophageal cells. Sci Rep, 6, 30593. doi:10.1038/srep30593

3. Zhou, M., Li, Y., Lin, S., Chen, Y., Qian, Y., Zhao, Z., & Fan, H. (2019). H3K9me3, H3K36me3, and H4K20me3 Expression Correlates with Patient Outcome in Esophageal Squamous Cell Carcinoma as Epigenetic Markers. Dig Dis Sci, 64(8), 2147-2157. doi:10.1007/s10620-019-05529-2

4. Li, Y., Guo, D., Sun, R., Chen, P., Qian, Q., & Fan, H. (2019). Methylation Patterns of Lys9 and Lys27 on Histone H3 Correlate with Patient Outcome in Gastric Cancer. Dig Dis Sci, 64(2), 439-446. doi:10.1007/s10620-018-5341-8

Alternate Names

H3F3A

Entrez Gene IDs

8290 (Human)

Gene Symbol

H3-4

Additional Histone H3 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Epigenetics
Interferons
Notch Pathway

Citations for Histone H3 [Citrulline Arg17, Citrulline Arg2, Citrulline Arg8] Antibody

Customer Reviews (3)

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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Histone H3 [Citrulline Arg17, Citrulline Arg2, Citrulline Arg8] Antibody
    Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: Brain tissue
    Species: Mouse
    Verified Customer | Posted 03/11/2025
    Nucleus (Blue, DAPI) ; H3cit (green) Hemorrhagic stroke model FxFr ; 10 µm thickness 1:400 Secondary antibody AF488
    Nucleus (Blue, DAPI) ; H3cit (green) Hemorrhagic stroke model FxFr ; 10 µm thickness 1:400 Secondary antibody AF488
    Histone H3 [Citrulline Arg17, Citrulline Arg2, Citrulline Arg8] Antibody NB100-57135
  • Histone H3 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: mouse hepatocytes and HepG2 cells
    Species: Human and Mouse
    Verified Customer | Posted 11/25/2017
    Histone H3 [Citrulline Arg17, Citrulline Arg2, Citrulline Arg8] Antibody NB100-57135
  • Name: Anonymous
    Application: Western Blot
    Sample Tested:
    Species: Human
    Verified Customer | Posted 08/06/2016
    Histone H3 [Citrulline Arg17, Citrulline Arg2, Citrulline Arg8] Antibody NB100-57135

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Protocols

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FAQs

Showing  1 - 3 of 3 FAQs Showing All

  • Q: Can you provide NB100-57135 biotynilated?

    A: Unfortunately, item NB100-57135 cannot be offered in a directly conjugated format as the base product is sold as unpurified antisera.

  • Q: What is the source of histone material used for the western blot with NB100-57135?

    A: NB100-57135 has been validated in western blot with HL60 cells treated with DMSO and calcimycin to induce citrullination.

  • Q: Would you please advise us what samples you used to generate WB image with NB100-57135?

    A: Our lab has just confirmed NB100-57135 has been validated in Western blot with HL60 cells treated with DMSO and calcimycin to induce citrullination.

  • Q: Can you provide NB100-57135 biotynilated?

    A: Unfortunately, item NB100-57135 cannot be offered in a directly conjugated format as the base product is sold as unpurified antisera.

  • Q: What is the source of histone material used for the western blot with NB100-57135?

    A: NB100-57135 has been validated in western blot with HL60 cells treated with DMSO and calcimycin to induce citrullination.

  • Q: Would you please advise us what samples you used to generate WB image with NB100-57135?

    A: Our lab has just confirmed NB100-57135 has been validated in Western blot with HL60 cells treated with DMSO and calcimycin to induce citrullination.

  • Q: Can you provide NB100-57135 biotynilated?

    A: Unfortunately, item NB100-57135 cannot be offered in a directly conjugated format as the base product is sold as unpurified antisera.

  • Q: What is the source of histone material used for the western blot with NB100-57135?

    A: NB100-57135 has been validated in western blot with HL60 cells treated with DMSO and calcimycin to induce citrullination.

  • Q: Would you please advise us what samples you used to generate WB image with NB100-57135?

    A: Our lab has just confirmed NB100-57135 has been validated in Western blot with HL60 cells treated with DMSO and calcimycin to induce citrullination.

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