hnRNP A2B1 Antibody (DP3B3) - BSA Free

Novus Biologicals | Catalog # NB120-6102

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine, Canine, Chicken, Primate

Cited:

Human, Mouse, Rat

Applications

Validated:

Knockout Validated, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Knockdown Validated

Cited:

Knockout Validated, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chemotaxis, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # DP3B3

Format

BSA Free
View all hnRNP A2B1 Primary Antibodies »

Product Specifications

Immunogen

Bacterially expressed human hnRNP A2 fusion protein.

Reactivity Notes

Mouse, Human, Chicken, Canine, Rat, Monkey, Bovine and Hamster.

Localization

Nuclear

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for hnRNP A2B1 Antibody (DP3B3) - BSA Free

Knockdown Validated: hnRNP A2B1 Antibody (DP3B3) - BSA Free [NB120-6102]

Western Blot: hnRNP A2B1 Antibody (DP3B3) - BSA Free [NB120-6102]

Western Blot: hnRNP A2B1 Antibody (DP3B3) [NB120-6102] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and hnRNP A1 knockout (KO) HEK293T cell line. PVDF membrane was probed with 1.0 ug/ml of Mouse Anti-Human hnRNP A1 Monoclonal Antibody (Catalog # NB120-6102) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for hnRNP A1 at approximately 35 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.
hnRNP A2B1 Antibody (DP3B3) - BSA Free

Western Blot Shows Human hnRNP A2B1 Specificity Using Knockout Cell Line.

Western blot shows lysates of HAP1 human near-haploid cell line and hnRNP A2B1 knockout HAP1 cell line (KO). Nitrocellulose membrane was probed with hnRNP A2B1 Antibody (Catalog # NB120-6102) O/N at 4C, followed by HRP-conjugated Secondary Antibody and ECL detection. A specific band was detected for hnRNP A2B1 (as indicated) in the parental HAP1 cell line, but is not detectable in knockout HAP1 cell line. Primary antibody dilution used: 1/1000. The Ponceau stained transfers of each blot are shown. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).
hnRNP A2B1 Antibody (DP3B3) - BSA Free

Knockout Validation of hnRNP A2B1 Antibody in HAP1 and hnRNP A2B1 Knockout HAP1 Cells in Immunocytochemistry/Immunofluorescence.

HAP1 WT and hnRNP A2B1 KO cells were labelled with a green or a far-red fluorescent dye, respectively. Cells were stained with hnRNP A2B1 antibody and with the corresponding Alexa-fluor 555 coupled secondary antibody including DAPI. Acquisition of the blue (nucleus-DAPI), green (identification of WT cells), red (antibody staining) and far-red (identification of KO cells) channels was performed. Representative images of the blue and red (grayscale) channels are shown. WT and KO cells are outlined with green and magenta dashed line, respectively. Antibody dilution used: 1/1000. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).
Western Blot: hnRNP A2B1 Antibody (DP3B3)BSA Free [NB120-6102]

Western Blot: hnRNP A2B1 Antibody (DP3B3)BSA Free [NB120-6102]

Western Blot: hnRNP A2B1 Antibody (DP3B3) [NB120-6102] - Whole cell protein from PC12 (lane 1), NIH-3T3 (lane 2), A431 (lane 3) and HeLa (lane 4) was separated by SDS-PAGE and transferred to PVDF membrane. The membrane was probed with Anti-hnRNP A2B1 at 1 ug/ml and detected with an HRP conjugated anti-mouse secondary antibody by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: hnRNP A2B1 Antibody (DP3B3) - BSA Free [NB120-6102]

Immunocytochemistry/ Immunofluorescence: hnRNP A2B1 Antibody (DP3B3) - BSA Free [NB120-6102]

Immunocytochemistry/Immunofluorescence: hnRNP A2B1 Antibody (DP3B3) [NB120-6102] - The hnRNP A2B1 antibody was tested in HeLa cells at a 5ug/ml using a Dylight 488 conjugated secondary antibody (Green). Actin (Red) and DNA (Blue) were counterstained using Phalloidin 568 and DAPI.
Western Blot: hnRNP A2B1 Antibody (DP3B3)BSA Free [NB120-6102]

Western Blot: hnRNP A2B1 Antibody (DP3B3)BSA Free [NB120-6102]

Western Blot: hnRNP A2B1 Antibody (DP3B3) [NB120-6102] - hnRNP A2B1 expression in HeLa cells. Lane 1= 15 ug of HeLa nuclear extracts Lane 2= Co-IP of hnRNP A2B1. Image and data from confirmed customer review.
Western Blot: hnRNP A2B1 Antibody (DP3B3)BSA Free [NB120-6102]

Western Blot: hnRNP A2B1 Antibody (DP3B3)BSA Free [NB120-6102]

Western Blot: hnRNP A2B1 Antibody (DP3B3) [NB120-6102] - Cell line lysates were separated on SDS-PAGE and probed with 2 ug/mL Monoclonal Anti-hnRNP-A2/B1 Clone: DP3B3. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. Lanes 1. HEK-293T 2. HeLa 3. G361 4. MDBK 5. MDCK 6. CHO
hnRNP A2B1 Antibody (DP3B3) - BSA Free

Western Blot: hnRNP A2B1 Antibody (DP3B3) - BSA Free [NB120-6102] -

Coilin suppression dysregulates expression of METTL3 and hnRNPA2B1. siRNAs were transfected into cells for 72 h. RNA or protein extracts were collected and subject to qRT-PCR or Western blot, respectively. For qRT-PCR (A,D), targets included GAPDH, METTL3, and hnRNPA2B1. For Western blot (B-C, E-F), signal intensities were determined for the targets (METTL3, hnRNPA2B1) and Beta-Actin. A target/actin ratio was calculated and normalized to control with control set to 1. All qRT-PCR data represent three biological replicates with two to three technical repeats (N=8-9). All Western blot data represent at least three biological replicates with one to two technical replicates (N=5-8). Error bars represent s.d. and black points represent individual data points. *P<0.05, ***P<0.001, ****P<0.0001, ns=not significant. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38050869), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for hnRNP A2B1 Antibody (DP3B3) - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation

reported in scientific literature (PMID 31844323)

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Immunoprecipitation

1:10-1:500

Knockout Validated

reported in scientific literature (PMID 31844323)

Western Blot

1 ug/ml

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: hnRNP A2B1

RNA polymerase II transcripts in the nucleus are in complex with several different proteins called heterogeneous nuclear ribonucleoproteins (hnRNPs). These proteins act in biological activities such as transcription, pre-mRNA processing, cytoplasmic mRNA ranslation, and turnover.2. hnRNPs can be isolated either by immunoprecipitation or by sucrose gradient fractionation of cell extracts. Isolated hnRNPs consist of protein groups named A to U and many of these protein groups consist of more than one isoform.3 The major steadystate proteins of the isolated hnRNP complex are the A1, A2, B1, B2, C1, and C2 with a range of molecular weights (34-43 kDa).3 hnRNP-A2/B1 proteins are located in the nucleus of most tissues cells; however, the hnRNP-A2 protein is found also in the cytoplasm of skin and esophagus cells. In rat brain, hnRNP-A2/B1 proteins are found in neurons in the cerebral cortices, hippocampal formation, olfactory regions, caudate-putamin, and the supraoptic ucleus of the hypothalamus.4 Expression of hnRNP-A2/B1 proteins was found also in lung development and its over expression correlated with lung cancer.5

Alternate Names

DKFZp779B0244, FLJ22720, heterogeneous nuclear ribonucleoprotein A2/B1, heterogeneous nuclear ribonucleoprotein B1, heterogeneous nuclear ribonucleoproteins A2/B1, hnRNP A2 / hnRNP B1, HNRNPA2, HNRNPB1, HNRPA2, HNRPA2B1hnRNP A2/B1, HNRPB1, nuclear ribonucleoprotein particle A2 protein, RNPA2, SNRPB1

Entrez Gene IDs

3181 (Human); 53379 (Mouse); 362361 (Rat)

Gene Symbol

HNRNPA2B1

UniProt

P22626 (Human), O88569 (Mouse), B2GV69 (Rat)

Additional hnRNP A2B1 Products

Product Documents for hnRNP A2B1 Antibody (DP3B3) - BSA Free

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Product Specific Notices for hnRNP A2B1 Antibody (DP3B3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for hnRNP A2B1 Antibody (DP3B3) - BSA Free

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Protocols

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