Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Chicken, Fish, Hamster, Rabbit

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Microarray

Cited:

Western Blot, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # H9010

Format

BSA Free
View all HSP90 beta Primary Antibodies »

Product Specifications

Immunogen

Recombinant human HSP90beta

Localization

Cytoplasm, Melanosome

Marker

ER Stress Marker

Specificity

Detects 90 kDa protein. Detects HSP90 beta in all reactive species except in Chicken, where it detects both alpha and beta isoforms.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for HSP90 beta Antibody (H9010) - BSA Free

Western Blot: HSP90 beta Antibody (H9010) [NB110-61640]

Western Blot: HSP90 beta Antibody (H9010) [NB110-61640]

Western Blot: HSP90 beta Antibody (H9010) [NB110-61640] - Western blot analysis of Human Lysates showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 (NB110-61640). Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (NB110-61640) at 1:1000. Comparison of clone H9010 behavior with Hsp90 human beta (1) and Hsp90 human alpha (2). Courtesy of: David Toft, Mayo Clinic.
Immunocytochemistry/ Immunofluorescence: HSP90 beta Antibody (H9010) [NB110-61640]

Immunocytochemistry/ Immunofluorescence: HSP90 beta Antibody (H9010) [NB110-61640]

Immunocytochemistry/Immunofluorescence: HSP90 beta Antibody (H9010) [NB110-61640] - Tissue: backskin. Species: Mouse. Fixation: Bouins Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.
Immunohistochemistry: HSP90 beta Antibody (H9010) [NB110-61640]

Immunohistochemistry: HSP90 beta Antibody (H9010) [NB110-61640]

Immunohistochemistry: HSP90 beta Antibody (H9010) [NB110-61640] - Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 (NB110-61640). Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (NB110-61640) at 1:10000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 l for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications..
Western Blot: HSP90 beta Antibody (H9010) [NB110-61640]

Western Blot: HSP90 beta Antibody (H9010) [NB110-61640]

Western Blot: HSP90 beta Antibody (H9010) [NB110-61640] - Human cell lysates from various cell lines showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010. 15 ug protein each lane. Block: 1.5% BSA for 30 minutes at RT. Primary antibody at 1:1000 for 2 hours at RT. Secondary antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Western Blot: HSP90 beta Antibody (H9010) [NB110-61640]

Western Blot: HSP90 beta Antibody (H9010) [NB110-61640]

Western Blot: HSP90 beta Antibody (H9010) [NB110-61640] - Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 (NB110-61640). Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (NB110-61640) at 1:1000. Secondary Antibody: HRP Goat Anti-Mouse.
Immunohistochemistry: HSP90 beta Antibody (H9010) [NB110-61640]

Immunohistochemistry: HSP90 beta Antibody (H9010) [NB110-61640]

Immunohistochemistry: HSP90 beta Antibody (H9010) [NB110-61640] - Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 (NB110-61640). Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (NB110-61640) at 1:10000 for 12 hours at 4C. Secondary Antibody: Alexa Fluor 555 Goat Anti-Mouse (red) at 1:5000 for 1 hour at RT. Localization: Inflammatory and epithelial mucosa. Magnification: 40x. Inflammatory and epithelial mucosa. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications..
Immunohistochemistry: HSP90 beta Antibody (H9010) [NB110-61640]

Immunohistochemistry: HSP90 beta Antibody (H9010) [NB110-61640]

Immunohistochemistry: HSP90 beta Antibody (H9010) [NB110-61640] - Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 (NB110-61640). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (NB110-61640) at 1:10000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 l for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications..
Immunohistochemistry: HSP90 beta Antibody (H9010) [NB110-61640]

Immunohistochemistry: HSP90 beta Antibody (H9010) [NB110-61640]

Immunohistochemistry: HSP90 beta Antibody (H9010) [NB110-61640] - Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 (NB110-61640). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (NB110-61640) at 1:10000 for 12 hours at 4C. Secondary Antibody: Alexa Fluor 555 Goat Anti-Mouse (red) at 1:5000 for 1 hour at RT. Magnification: 40x. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications..

Applications for HSP90 beta Antibody (H9010) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:100

Western Blot

1:2500
Application Notes
1 ug/ml of HSP90 antibody was sufficient for detection of HSP90beta in 20 ug of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Reviewed Applications

Read 5 reviews rated 4.8 using NB110-61640 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.2), 50% Glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HSP90 beta

HSP90 is an abundantly and ubiquitously expressed heat shock protein. It is understood to exist in two principal forms alpha and beta, which share 85% sequence amino acid homology. The two isoforms of Hsp90, are expressed in the cytosolic compartment (1). Despite the similarities, HSP90alpha exists predominantly as a homodimer while HSP90beta; exists mainly as a monomer.(2) From a functional perspective, hsp90 participates in the folding, assembly, maturation, and stabilization of specific proteins as an integral component of a chaperone complex. (3-6) Furthermore, Hsp90 is highly conserved between species; having 60% and 78% amino acid similarity between mammalian and the corresponding yeast and Drosophila proteins, respectively. Hsp90 is a highly conserved and essential stress protein that is expressed in all eukaryotic cells. Despite it's label of being a heat-shock protein, hsp90 is one of the most highly expressed proteins in unstressed cells (1'2% ofcytosolic protein). It carries out a number of housekeeping functions including controlling the activity, turnover, and trafficking of a variety of proteins. Most of the hsp90- regulated proteins that have been discovered to date are involved in cell signaling. (7-8). The number of proteins now know to interact with Hsp90 is about 100. Target proteins include the kinases v-Src, Wee1, and c-Raf, transcriptional regulators such as p53 and steroid receptors, and the polymerases of the hepatitis B virus and telomerase.5 When bound to ATP, Hsp90 interacts with co-chaperones Cdc37, p23, and an assortment of immunophilin-like proteins, forming a complex that stabilizes and protects target proteins from proteasomal degradation. In most cases, hsp90-interacting proteins have been shown to co-precipitate with hsp90 when carrying out immunoadsorption studies, and to exist in cytosolic heterocomplexes with it. In a number of cases, variations in hsp90 expression or hsp90 mutation has been shown to degrade signaling function via the protein or to impair a specific function of the protein (such as steroid binding, kinase activity) in vivo. Ansamycin antibiotics, such as geldanamycin and radicicol, inhibit hsp90 function (9).

Long Name

Heat Shock Protein 90 beta

Alternate Names

D6S182, HSP84, HSP90AB1, HSP90B, HSPC2, HSPCB

Gene Symbol

HSP90AB1

Additional HSP90 beta Products

Product Documents for HSP90 beta Antibody (H9010) - BSA Free

Certificate of Analysis

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Product Specific Notices for HSP90 beta Antibody (H9010) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for HSP90 beta Antibody (H9010) - BSA Free

Customer Reviews for HSP90 beta Antibody (H9010) - BSA Free (5)

4.8 out of 5
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Customer Images

Showing  1 - 5 of 5 reviews Showing All
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  • HSP90 beta Antibody (H9010)
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Human liver cancer cell line (HuH7) and HepG2
    Species: Human
    Verified Customer | Posted 04/04/2020
    HSP90 beta Antibody (H9010) - BSA Free NB110-61640
  • HSP90 beta Antibody (H9010)
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Breast cancer tissue
    Species: Human
    Verified Customer | Posted 04/04/2020
    HSP90 beta Antibody (H9010) - BSA Free NB110-61640
  • HSP90 beta Antibody (H90-10)
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Breast cancer tissue
    Species: Human
    Verified Customer | Posted 02/19/2019
    HSP90 beta Antibody (H9010) - BSA Free NB110-61640
  • HSP90AB1 Antibody (H90-10)
    Name: Anonymous
    Application: Western Blot
    Sample Tested: hela cell and 293T
    Species: Human
    Verified Customer | Posted 12/19/2018
    HSP90 beta Antibody (H9010) - BSA Free NB110-61640
  • HSP90AB1 Antibody (H90-10)
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HCC827 human non-small cell lung cancer cell line and A549 cells
    Species: Human
    Verified Customer | Posted 10/17/2018
    HSP90 beta Antibody (H9010) - BSA Free NB110-61640

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Protocols

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Associated Pathways

Apoptosis Signaling Pathway Apoptosis Signaling Pathway Thumbnail