Detection of Human ABCB5 by Western Blot.
Western blot shows lysates of Bowes human melanoma cell line and K562 human chronic myelogenous leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Human ABCB5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5799) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ABCB5 at approximately 100-110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
ABCB5 (ATP-binding cassette, subfamily B [DR/TAP] member 5) is a 90 kDa (predicted MW) member of the human P-glycoprotein family of molecules. It is expressed by CD133+ pluripotent pigment stem cells, where it serves as a marker for cells that show a high incidence of polyploidy. Human ABCB5 is 812 amino acids (aa) in length. It is possibly a five transmembrane protein with a 247 aa N-terminal extracellular domain (ECD) (aa 1-247) and a 283 aa C-terminal cytoplasmic region (aa 530-812). The ECD contains one ABC transporter (aa 2-177), while the cytoplasmic region contains a second ABC transporter (aa 570-808). There are two potential splice forms. One shows a seven aa substitution for aa 125-812, while another possesses an alternative start site 445 aa upstream of the standard site. Over aa 141-247, human ABCB5 shares 64% aa identity with mouse ABCB5.
ATP-binding Cassette, Sub-family B (MDR/TAP), Member 5
Entrez Gene IDs:
340273 (Human); 77706 (Mouse); 314537 (Rat)
ABCB5 P-gp; ABCB5; ABCB5alpha; ABCB5beta; ATP-binding cassette protein; ATP-binding cassette sub-family B member 5; ATP-binding cassette, sub-family B (MDR/TAP), member 5; EC 3.6.3; EC 126.96.36.199; EST422562; P-glycoprotein ABCB5
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