Human ACE Quantikine ELISA Kit

Catalog # Availability Size / Price Qty
DACE00
Control Products Available
Human ACE/CD143 ELISA Cell Culture Supernate/Saliva Standard Curve
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Product Details
Procedure
Citations (4)
FAQs
Supplemental Products
Reviews

Human ACE Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), Heparin Plasma (10 uL), Saliva (25 uL)
Sensitivity
0.051 ng/mL
Assay Range
0.8 - 50 ng/mL (Cell Culture Supernates, Serum, Heparin Plasma, Saliva)
Specificity
Natural and recombinant human ACE. In addition to soluble ACE, this kit recognizes membrane-bound forms from lysates of cultured cells and supernates.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human ACE Immunoassay is a 4.5 hour solid phase ELISA designed to measure ACE in cell culture supernates, serum, plasma, and saliva. It contains NS0-expressed ectodomain of recombinant human sACE, and antibodies raised against the recombinant factor. Natural human ACE showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards, indicating that this kit can be used to determine relative levels of natural human ACE.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Saliva

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (ng/mL) 3.6 10.7 22.1 3.44 10.3 21.8
Standard Deviation 0.08 0.3 0.95 0.21 0.4 0.87
CV% 2.2 2.8 4.3 6.1 3.9 4

Serum, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (ng/mL) 3.57 10.9 22.5 3.77 11.7 24.4
Standard Deviation 0.12 0.43 0.9 0.29 0.6 1.2
CV% 3.4 3.9 4 7.7 5.1 4.9

Recovery

The recovery of ACE spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=4) 98 89-106
Heparin Plasma (n=4) 100 94-106
Serum (n=4) 99 91-106

Linearity

To assess the linearity of the assay, samples containing high concentrations of ACE were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human ACE/CD143 ELISA Linearity

Data Examples

Human ACE/CD143 ELISA Cell Culture Supernate/Saliva Standard Curve

Human ACE/CD143 ELISA Serum/Plasma Standard Curve

Product Datasheets

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: ACE/CD143

ACE and ACE-2, two cell surface proteases, are important regulators of the renin-angiotensin system (RAS), which plays a key role in maintaining blood pressure homeostasis and fluid salt balance in mammals. Because of its location and specificity, ACE plays additional roles in immunity, reproduction and neuropeptide regulation. ACE exists in two isoforms. Somatic ACE comprises two highly similar protease domains whereas germinal ACE comprises a single protease domain. ACE-2 consists of a single protease domain and is an essential regulator of heart and lung function. It is also a cellular receptor for the Spike protein of SARS cornonavirus that causes severe acute respiratory syndrome.

Long Name:
Angiotensin I Converting Enzyme
Entrez Gene IDs:
1636 (Human); 11421 (Mouse)
Alternate Names:
ACE; ACE1angiotensin converting enzyme, somatic isoform; angiotensin I converting enzyme (peptidyl-dipeptidase A) 1; carboxycathepsin; CD143 antigen; CD143; DCP; DCP1; DCP1angiotensin-converting enzyme; dipeptidyl carboxypeptidase 1; Dipeptidyl carboxypeptidase I; EC 3.2.1.-; EC 3.4.15.1; Kininase II; MGC26566; MVCD3; peptidase P; testicular ECA
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human ACE Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. RhoA-Rho associated kinase signaling leads to renin-angiotensin system imbalance and angiotensin converting enzyme 2 has a protective role in acute pulmonary embolism
    Authors: X Xu, L Shi, X Ma, H Su, G Ma, X Wu, K Ying, R Zhang
    Thromb. Res., 2019;176(0):85-94.
    Species: Human
    Sample Types: Plasma
  2. Associations of Angiotensin Converting Enzyme-1 and Angiotensin II Blood Levels and Cognitive Function
    Authors: S Yasar, VR Varma, GC Harris, MC Carlson
    J. Alzheimers Dis., 2018;0(0):.
    Species: Human
    Sample Types: Serum
  3. Circulating ACE is a predictor of weight loss maintenance not only in overweight and obese women, but also in men.
    Authors: Wang P, Holst C, Wodzig W, Andersen M, Astrup A, van Baak M, Larsen T, Jebb S, Kafatos A, Pfeiffer A, Martinez J, Handjieva-Darlenska T, Kunesova M, Viguerie N, Langin D, Saris W, Mariman E
    Int J Obes (Lond), 2012;36(12):1545-51.
    Species: Human
    Sample Types: Serum
  4. Soluble adhesion molecules and angiotensin-converting enzyme in dementia.
    Authors: Nielsen HM, Londos E, Minthon L, Janciauskiene SM
    Neurobiol. Dis., 2007;26(1):27-35.
    Species: Human
    Sample Types: Plasma

FAQs

  1. Is ACE-1 the same as ACE?

    • Yes. ACE-1 is used as an alternative name, but not as commonly as ACE and CD143.

View all ELISA FAQs

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