Human ADAMTS13 Antibody

Discontinued Product

MAB4245 has been discontinued.
View all ADAMTS13 products.
Detection of ADAMTS13 in HUVEC Human Cells by Flow Cytometry.
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Human ADAMTS13 Antibody Summary

Species Reactivity
Human
Specificity
Detects human ADAMTS13 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) ADAMTS1, rhADAMTS4, rhADAMTS5, or rhADAMTS-L1.2 is observed.
Source
Monoclonal Mouse IgG1 Clone # 442323
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human ADAMTS13 isoform 1
Gln34-Trp688 (predicted)
Accession # Q76LX8
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human ADAMTS13 (Catalog # 4245-AD) under non-reducing conditions only
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human ADAMTS13 Full Length (Catalog # 6156-AD), see our available Western blot detection antibodies
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection of ADAMTS13 antibody in HUVEC Human Cells antibody by Flow Cytometry. View Larger

Detection of ADAMTS13 in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human ADAMTS13 Monoclonal Antibody (Catalog # MAB4245, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ADAMTS13

ADAMTS13 (a disintegrin and metalloproteinase with thrombospondin motifs 13) is a secreted multi-domain zinc protease primarily produced by the liver. In the circulation, it is responsible for cleaving von Willebrand factor (vWF) between Tyr842 and Met843. Defects in ADAMTS13 activity result in the generation of ultra large vWF, leading to thrombotic thrombocytopenic purpura (TTP). The amino acid sequence of human ADAMTS13 in the region of aa 34 to 688 is 76% identical to that of mouse/rat.

Long Name
A Disintegrin-like and Metalloproteinase Domain with Thrombospondin Motifs 13
Entrez Gene IDs
11093 (Human); 279028 (Mouse); 362091 (Rat)
Alternate Names
A disintegrin and metalloproteinase with thrombospondin motifs 13; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondintype 1 motif, 13; ADAM metallopeptidase with thrombospondin type 1 motif, 13; ADAMTS13; ADAM-TS13; ADAMTS-13; DKFZp434C2322; EC 3.4.24.14; EC 3.4.24.82; EC 3.4.24.87; FLJ42993; MGC118899; MGC118900; TTP; TTPADAM-TS 13; vWF-cleaving protease; vWF-CP; vWF-CPC9orf8; VWFCPvon Willebrand factor-cleaving protease

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

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Reviews for Human ADAMTS13 Antibody

Average Rating: 3.7 (Based on 3 Reviews)

5 Star
0%
4 Star
66.67%
3 Star
33.33%
2 Star
0%
1 Star
0%

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Human ADAMTS13 Antibody
By Anonymous on 03/11/2019
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human ADAMTS13 Antibody
By Anonymous on 02/21/2019
Application: Microarray Sample Tested: EDTA Plasma Species: Human

Human ADAMTS13 Antibody
By Anonymous on 11/11/2018
Application: ELISA Sample Tested: Serum and Plasma Species: Human