Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all ADAMTS13 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human ADAMTS13
Gln34-Trp688
Accession # Q76LX8

Specificity

Detects human ADAMTS13 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant human (rh) ADAMTS14 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Human ADAMTS13 Antibody

Application
Recommended Usage

ELISA

This antibody functions as an ELISA detection antibody when paired with anti-Human ADAMTS13 monoclonal antibody (Catalog # MAB42451).This product is intended for assay development on various assay platforms requiring antibody pairs.

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human ADAMTS13 Full Length (Catalog # 6156-AD), see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Human ADAMTS13 (Catalog # 4245-AD)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ADAMTS13

ADAMTS13 (A disintegrin and metalloprotease with TSP motifs 13) is a 140-230 kDa, secreted glycoprotein member of the ADAMTS family of proteases. ADAMTS13 is produced by the liver and circulates in plasma where it cleaves and reduces the activity of von Willebrand factor. ProADAMTS13 is 1398 amino acids (aa) in length. It contains a 45 aa prodomain (aa 30-74), one peptidase domain (aa 80-286) and one disintegrin (aa 287-383) domain, multiple TSP regions and two CUB domains. The prodomain does not affect activity, while the TSP and CUB domains appear to be necessary for activity. One truncated form exists that shows truncation after Gln448. Over aa 34-688, human ADAMST13 shares 76% aa identity with mouse ADAMST13.

Long Name

A Disintegrin-like and Metalloproteinase Domain with Thrombospondin Motifs 13

Alternate Names

TTP, vWF-CP

Entrez Gene IDs

11093 (Human); 279028 (Mouse); 362091 (Rat)

Gene Symbol

ADAMTS13

UniProt

Q76LX8

Additional ADAMTS13 Products

Product Documents for Human ADAMTS13 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human ADAMTS13 Antibody

For research use only

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