Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA Detection (Matched Antibody Pair)

Cited:

Antibody Array Development, ELISA Detection, ELISA Development, Functional Assay, Luminex Development

Label

Biotin

Antibody Source

Monoclonal Mouse IgG1 Clone # 166128
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Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Adiponectin
Glu19-Asn244
Accession # Q15848

Specificity

Detects human Adiponectin in ELISAs. In ELISAs, this antibody shows no cross-reactivity with recombinant mouse Adiponectin. This antibody detects an epitope in the globular domain (amino acids 104 - 244).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human Adiponectin/Acrp30 Biotinylated Antibody

Human Adiponectin/Acrp30 Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.5-2.0 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Human Adiponectin/Acrp30 Antibody (Catalog # MAB10651)
  • Capture Reagent: Human Adiponectin/Acrp30 Antibody (Catalog # MAB10651R)
  • Standard: Recombinant Human Adiponectin/Acrp30 Protein, CF (Catalog # 1065-AP)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.


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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Adiponectin/Acrp30

Adiponectin, also known as adipocyte complement-related protein of 30 kDa (Acrp30), is secreted from adipocytes and has been implicated in energy homeostasis and obesity.

Alternate Names

Acrp30, AdipoQ, ApM1, GBP28

Entrez Gene IDs

9370 (Human); 11450 (Mouse); 246253 (Rat)

Gene Symbol

ADIPOQ

UniProt

Additional Adiponectin/Acrp30 Products

Product Documents for Human Adiponectin/Acrp30 Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Adiponectin/Acrp30 Biotinylated Antibody

For research use only

Citations for Human Adiponectin/Acrp30 Biotinylated Antibody

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FAQs

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