Human Aggrecan G1-IGD-G2 Domains Antibody Summary
Accession # NP_037359
under non-reducing conditions only
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Aggrecan in Human Cartilage. Aggrecan was detected in immersion fixed paraffin-embedded sections of human cartilage using Mouse Anti-Human Aggrecan G1-IGD-G2 Domains Monoclonal Antibody (Catalog # MAB1220) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to chondrocytes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Aggrecan, also known as aggrecan 1, chondroitin sulfate proteoglycan, and large aggregating proteoglycan, is encoded by the AGC1 gene with gene aliases of SEDK; CSPG1; MSK16; CSPGCP (1). As the key component of the cartilage extracellular matrix, aggrecan hydrates the collagen network and provides cartilage with its properties of compressibility and elasticity. Maintenance of aggrecan content is therefore critical to the function of the tissue and aggrecan degradation is an important factor in the erosion of articular cartilage in arthritic diseases (2). The deduced amino acid sequence of human aggrecan core protein consists of 2415 residues and predicts a signal peptide and domains of G1, IGD, G2, KS, CS-1, CS-2 and G3 (3). Two globular domains, G1 and G2, comprise the N-terminus of the proteoglycan and also contain link domains. The third globular domain, G3, corresponds to the C-terminus. The keratan sulfate (KS) and the chondroitin sulfate (CS) attachment domains are between G2 and G3. With KS and CS attached to the 250 kDa core protein, aggrecan monomers exist as a 1,000 to 2,000 kDa molecule. In addition, aggrecan monomers interact with hyaluronan through their G1 domain, resulting in larger aggregates containing 10 to 100 aggrecan monomers on a hyaluronan backbone (2). Aggrecan can be cleaved by MMPs and ADAMTSs at the Asn360-Phe361 and Glu392-Ala393 bond in the IGD (residues are numbered based on Accession # NP_037359), respectively (2). Inhibition of ADATMS4 and ADAMTS5 cleavage prevents aggrecan degradation in osteoarthritic cartilage, while mice with aggrecan resistant to MMP cleavage do not accumulate aggrean and develop normally (2, 4).
- Doege. K.J. et al. (1991) J. Biol. Chem. 266:894.
- Malfait, A.-M. et al. (2002) J. Biol. Chem. 277:22201.
- Caterson, B. et al. (2000) Matrix Biol. 19:333.
- Little, C.B. et al. (2005) Mol. Cel. Biol. 25:3388.
Citations for Human Aggrecan G1-IGD-G2 Domains Antibody
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Citations: Showing 1 - 3
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Influence of Conditioned Media on the Re-Differentiation Capacity of Human Chondrocytes in 3D Spheroid Cultures
Authors: A Klinder, S Kussauer, B Hiemer, A Wree, R Bader, A Jonitz-Hei
J Clin Med, 2020;9(9):.
Sample Types: Whole Tissue
LOXL2 Is Highly Expressed in Cancer-Associated Fibroblasts and Associates to Poor Colon Cancer Survival.
Authors: Torres S, Garcia-Palmero I, Herrera M, Bartolome R, Pena C, Fernandez-Acenero M, Padilla G, Pelaez-Garcia A, Lopez-Lucendo M, Rodriguez-Merlo R, Garcia de Herreros A, Bonilla F, Casal J
Clin Cancer Res, 2015;21(21):4892-902.
Sample Types: Whole Tissue
Interaction of cartilage oligomeric matrix protein/thrombospondin 5 with aggrecan.
Authors: Chen FH, Herndon ME, Patel N, Hecht JT, Tuan RS, Lawler J
J. Biol. Chem., 2007;282(34):24591-8.
Sample Types: Recombinant Protein
Applications: ELISA Development
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