Detection of Human alpha -N-terminal Methyltransferase 1A/ METTL11A by Western Blot.
Western blot shows lysates of U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human alpha ‑N‑terminal Methyltransferase 1A/METTL11A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7159) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for alpha ‑N‑terminal Methyltransferase 1A/ METTL11A at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
METTL11A, also named N-terminal RCC1 methyltransferase (NRMT), is an alpha-N-methyltransferase that methylates the N-terminal amino group of target proteins containing the N-terminal motif [Ala/Pro/Ser]-Pro-Lys when the initiator Met is cleaved (1). It is responsible for N-terminal methylation of RCC1, KLHL31, MYL2, MYL3, RB1, RPL23A and SET. NRMT lacks a SET domain but possesses a Rossman-like a/b fold. The residues Asn169, Asp178, Asp181, and Ser183 of NRMT are important for substrate binding (2). RCC1 (Ran guanine nucleotide-exchange factor) is the first protein for which any biological function of alpha-N-methylation by NRMT has been identified (3, 4). The multi-spindle phenotype associated with either NRMT knockdown or methylation-defective RCC1 mutants demonstrated the importance of alpha-N-methylation of RCC1 for normal bipolar spindle formation and chromosome aggregation (2). NRMT is robustly overexpressed in gastrointestinal cancers.
Webb, K. J. et al. (2010) Biochemistry 49:5225.
Christine, S. E. et al. (2010) Nature 466:1125.
Chen, T. et al. (2007) Nature Cell Biol. 9:596.
Hao, Y. et al. (2008) J. Cell. Biol. 182:827.
alpha-N-terminal Protein Methyltransferase 1A
Entrez Gene IDs:
28989 (Human); 66617 (Mouse); 362103 (Rat)
AD-003; alpha N-terminal protein methyltransferase 1A; C9orf32; chromosome 9 open reading frame 32; EC 2.1.1.-; methyltransferase like 11A; Methyltransferase-like protein 11A; METTL11A; NRMT; N-terminal RCC1 methyltransferase; NTM1A; NTMT1; X-Pro-Lys N-terminal protein methyltransferase 1A
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