Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all alpha-N-terminal Methyltransferase 1A/METTL11A Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human alpha -N-terminal Methyltransferase 1A/METTL11A
Thr2-Arg223
Accession # Q9BV86

Specificity

Detects human alpha -N-terminal Methyltransferase 1A/METTL11A in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human alpha-N-terminal Methyltransferase 1A/METTL11A Antibody

Detection of Human a-N-terminal Methyltransferase 1A/ METTL11A antibody by Western Blot.

Detection of Human alpha -N-terminal Methyltransferase 1A/ METTL11A by Western Blot.

Western blot shows lysates of U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human a-N-terminal Methyltransferase 1A/METTL11A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7159) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for a-N-terminal Methyltransferase 1A/ METTL11A at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human alpha-N-terminal Methyltransferase 1A/METTL11A Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: U937 human histiocytic lymphoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: alpha-N-terminal Methyltransferase 1A/METTL11A

METTL11A, also named N‑terminal RCC1 methyltransferase (NRMT), is an alpha‑N‑methyltransferase that methylates the N‑terminal amino group of target proteins containing the N‑terminal motif [Ala/Pro/Ser]-Pro-Lys when the initiator Met is cleaved (1). It is responsible for N‑terminal methylation of RCC1, KLHL31, MYL2, MYL3, RB1, RPL23A and SET. NRMT lacks a SET domain but possesses a Rossman‑like a/b fold. The residues Asn169, Asp178, Asp181, and Ser183 of NRMT are important for substrate binding (2). RCC1 (Ran guanine nucleotide‑exchange factor) is the first protein for which any biological function of alpha‑N‑methylation by NRMT has been identified (3, 4). The multi‑spindle phenotype associated with either NRMT knockdown or methylation‑defective RCC1 mutants demonstrated the importance of alpha‑N‑methylation of RCC1 for normal bipolar spindle formation and chromosome aggregation (2). NRMT is robustly overexpressed in gastrointestinal cancers.

References

  1. Webb, K. J. et al. (2010) Biochemistry 49:5225.
  2. Christine, S. E. et al. (2010) Nature 466:1125.
  3. Chen, T. et al. (2007) Nature Cell Biol. 9:596.
  4. Hao, Y. et al. (2008) J. Cell. Biol. 182:827.

Long Name

alpha-N-terminal Protein Methyltransferase 1A

Alternate Names

AD-003, C9orf32, NRMT, NTM1A, NTMT1

Entrez Gene IDs

28989 (Human); 66617 (Mouse); 362103 (Rat)

Gene Symbol

NTMT1

UniProt

Q9BV86

Additional alpha-N-terminal Methyltransferase 1A/METTL11A Products

Product Documents for Human alpha-N-terminal Methyltransferase 1A/METTL11A Antibody

Certificate of Analysis

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Product Specific Notices for Human alpha-N-terminal Methyltransferase 1A/METTL11A Antibody

For research use only

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