Human alpha -N-terminal Methyltransferase 1A/ METTL11A Antibody

    
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  • Species Reactivity
    Human
  • Specificity
    Detects human alpha -N-terminal Methyltransferase 1A/METTL11A in direct ELISAs and Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human alpha -N-terminal Methyltransferase 1A/METTL11A Thr2-Arg223 Accession # Q9BV86
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of Human alpha -N-terminal Methyltransferase 1A/
METTL11A by Western Blot.

Western blot shows lysates of U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human alpha ‑N‑terminal Methyltransferase 1A/METTL11A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7159) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for alpha ‑N‑terminal Methyltransferase 1A/
METTL11A at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
      12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Background: alpha-N-terminal Methyltransferase 1A/METTL11A
METTL11A, also named N-terminal RCC1 methyltransferase (NRMT), is an alpha-N-methyltransferase that methylates the N-terminal amino group of target proteins containing the N-terminal motif [Ala/Pro/Ser]-Pro-Lys when the initiator Met is cleaved (1). It is responsible for N-terminal methylation of RCC1, KLHL31, MYL2, MYL3, RB1, RPL23A and SET. NRMT lacks a SET domain but possesses a Rossman-like a/b fold. The residues Asn169, Asp178, Asp181, and Ser183 of NRMT are important for substrate binding (2). RCC1 (Ran guanine nucleotide-exchange factor) is the first protein for which any biological function of alpha-N-methylation by NRMT has been identified (3, 4). The multi-spindle phenotype associated with either NRMT knockdown or methylation-defective RCC1 mutants demonstrated the importance of alpha-N-methylation of RCC1 for normal bipolar spindle formation and chromosome aggregation (2). NRMT is robustly overexpressed in gastrointestinal cancers.
  • References:
    1. Webb, K. J. et al. (2010) Biochemistry 49:5225.
    2. Christine, S. E. et al. (2010) Nature 466:1125.
    3. Chen, T. et al. (2007) Nature Cell Biol. 9:596.
    4. Hao, Y. et al. (2008) J. Cell. Biol. 182:827.
  • Long Name:
    alpha-N-terminal Protein Methyltransferase 1A
  • Entrez Gene IDs:
    28989 (Human); 66617 (Mouse); 362103 (Rat)
  • Alternate Names:
    AD-003; alpha N-terminal protein methyltransferase 1A; C9orf32; chromosome 9 open reading frame 32; EC 2.1.1.-; methyltransferase like 11A; Methyltransferase-like protein 11A; METTL11A; NRMT; N-terminal RCC1 methyltransferase; NTM1A; NTMT1; X-Pro-Lys N-terminal protein methyltransferase 1A
Related Research Areas

FAQs

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Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 11
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Reconstitution Buffers
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Reconstitution Buffer 1 (PBS)

RB01
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB , Simple Western HAF016 18  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow , IHC , ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow , IHC , ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow , IHC , ICC NL012 3
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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