Human alpha-N-terminal Methyltransferase 1A/METTL11A Antibody

Catalog # Availability Size / Price Qty
AF7159
AF7159-SP
Detection of Human  alpha -N-terminal Methyltransferase 1A/METTL11A by Western Blot.
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Human alpha-N-terminal Methyltransferase 1A/METTL11A Antibody Summary

Species Reactivity
Human
Specificity
Detects human alpha -N-terminal Methyltransferase 1A/METTL11A in direct ELISAs and Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human alpha -N-terminal Methyltransferase 1A/METTL11A
Thr2-Arg223
Accession # Q9BV86
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human  alpha -N-terminal Methyltransferase 1A/METTL11A by Western Blot. View Larger

Detection of Human alpha -N-terminal Methyltransferase 1A/
METTL11A by Western Blot.

Western blot shows lysates of U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human alpha ‑N‑terminal Methyltransferase 1A/METTL11A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7159) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for alpha ‑N‑terminal Methyltransferase 1A/
METTL11A at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: alpha-N-terminal Methyltransferase 1A/METTL11A

METTL11A, also named N‑terminal RCC1 methyltransferase (NRMT), is an alpha‑N‑methyltransferase that methylates the N‑terminal amino group of target proteins containing the N‑terminal motif [Ala/Pro/Ser]-Pro-Lys when the initiator Met is cleaved (1). It is responsible for N‑terminal methylation of RCC1, KLHL31, MYL2, MYL3, RB1, RPL23A and SET. NRMT lacks a SET domain but possesses a Rossman‑like a/b fold. The residues Asn169, Asp178, Asp181, and Ser183 of NRMT are important for substrate binding (2). RCC1 (Ran guanine nucleotide‑exchange factor) is the first protein for which any biological function of alpha‑N‑methylation by NRMT has been identified (3, 4). The multi‑spindle phenotype associated with either NRMT knockdown or methylation‑defective RCC1 mutants demonstrated the importance of alpha‑N‑methylation of RCC1 for normal bipolar spindle formation and chromosome aggregation (2). NRMT is robustly overexpressed in gastrointestinal cancers.

References
  1. Webb, K. J. et al. (2010) Biochemistry 49:5225.
  2. Christine, S. E. et al. (2010) Nature 466:1125.
  3. Chen, T. et al. (2007) Nature Cell Biol. 9:596.
  4. Hao, Y. et al. (2008) J. Cell. Biol. 182:827.
Long Name
alpha-N-terminal Protein Methyltransferase 1A
Entrez Gene IDs
28989 (Human); 66617 (Mouse); 362103 (Rat)
Alternate Names
AD-003; alpha N-terminal protein methyltransferase 1A; C9orf32; chromosome 9 open reading frame 32; EC 2.1.1.-; methyltransferase like 11A; Methyltransferase-like protein 11A; METTL11A; NRMT; N-terminal RCC1 methyltransferase; NTM1A; NTMT1; X-Pro-Lys N-terminal protein methyltransferase 1A

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