Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Aminopeptidase P1/XPNPEP1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Aminopeptidase P1/XPNPEP1
Met1-His623
Accession # NP_065116

Specificity

Detects human Aminopeptidase P1/XPNPEP1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross‑reactivity with recombinant human (rh) XPNPEP2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human Aminopeptidase P1/XPNPEP1 Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Aminopeptidase P1/XPNPEP1 (Catalog # 2970-ZN), see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Human Aminopeptidase P1/XPNPEP1 (Catalog # 2970-ZN)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Aminopeptidase P1/XPNPEP1

The human XPNPEP1 gene encodes aminopeptidase P1 (APP1), which is also known as X-prolyl aminopeptidase with gene aliases of SAMP, XPNPEP, XPNPEPL, and XPNPEPL1 (1‑3). It is a member of the M24 family of metalloproteases, which also contains methionine aminopeptidases, X-Pro dipeptidase, aminopeptidase P2, aminopeptidase P homolog, proliferation-associated protein 1, and suppressor of Ty homolog or chromatin-specific transcription elongation factor large subunit (4). It is a soluble enzyme, in contrast to the GPI-anchored APP2 encoded by XPNPEP2 (5). Human APP1 is widely expressed (3). The purified rhAPP1 is an active aminopeptidase, removing a N‑terminal amino acid from a peptide that contains a Pro residue at the second position. The amino acid sequence of human APP1 is 99%, 97%, 95%, 74% and 73% identical to that of canine, bovine, mouse/rat, Xenopus and zebrafish, respectively.

References

  1. Cottrell, G.S. et al. (2000) Biochemistry 39:15121.
  2. Sprinkle, T.J. et al. (2000) Arch. Biochem. Biophys. 378:51.
  3. Vanhoof, G. et al. (1997) Cytogenet. Cell Genet. 78:275.
  4. Turner, A.J. and G.S. Cottrell (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, A.J. et al.) pp. 931, Elsevier Academic Press, San Diego. 
  5. Simmons, W.H. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, A.J. et al.) pp. 934, Elsevier Academic Press, San Diego. 

Alternate Names

APP1, SAMP, XPNPEP1

Entrez Gene IDs

7511 (Human)

Gene Symbol

XPNPEP1

UniProt

NP_065116

Additional Aminopeptidase P1/XPNPEP1 Products

Product Documents for Human Aminopeptidase P1/XPNPEP1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Aminopeptidase P1/XPNPEP1 Antibody

For research use only

Related Research Areas

Metalloproteases and Regulators

Citations for Human Aminopeptidase P1/XPNPEP1 Antibody

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