Human Apolipoprotein B/ApoB Alexa Fluor® 594-conjugated Antibody

Catalog #: AF3556T Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3556T-100UG

Save 15% on Select RUO Reagents. See Details.

Product Details

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Apolipoprotein B/ApoB Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Apolipoprotein B/ApoB in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Apolipoprotein B/ApoB
Met1206-Asp1413
Accession # NP_000375

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Apolipoprotein B/ApoB

ApoB is a component of LDL and VLDL particles and chylomicans. ApoB100, the full-length ApoB synthesized in the liver, is a 512 kDa secreted palmetoylated glycoprotein that contains 4536 amino acid residues. A truncated ApoB designated ApoB48, containing the N-terminal 48% of ApoB100, is also produced by the intestine as a result of post-transcriptional modification of the ApoB mRNA. Additionally, C-terminal truncated ApoB, varying in length from ApoB25 to ApoB89, exists as a result of mutations in the ApoB gene. Within the region used as the immunogen, the amino acid sequence of the human peptide is 80% and 82% identical to the mouse and canine sequence, respectively.

Entrez Gene IDs

338 (Human); 238055 (Mouse); 54225 (Rat)

Alternate Names

Apo B-100; APOB; ApoB-100; ApoB-48; apolipoprotein B (including Ag(x) antigen); Apolipoprotein B; apolipoprotein B-100; apolipoprotein B48; FLDB; LDLCQ4; mutant Apo B 100