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Human Apolipoprotein B/ApoB Antibody

Catalog #: AF3556
7 Citations Datasheet / COA / SDS
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AF3556
AF3556-SP

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Detection of Human Apolipoprotein B/ApoB by Western Blot.
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Citations (7)
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Human Apolipoprotein B/ApoB Antibody Summary

Species Reactivity
Human
Specificity
Detects human Apolipoprotein B/ApoB in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Apolipoprotein B/ApoB
Met1206-Asp1413
Accession # NP_000375
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
5 µg/mL
Human plasma
Immunohistochemistry
1-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Apolipoprotein B/ApoB antibody by Western Blot. View Larger

Detection of Human Apolipoprotein B/ApoB by Western Blot. Western blot shows human plasma. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Apolipoprotein B/ApoB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3556) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Apolipoprotein B/ApoB at approximately 500 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Apolipoprotein B/ApoB antibody in Human Liver by Immunohistochemistry (IHC-P). View Larger

Apolipoprotein B/ApoB in Human Liver. Apolipoprotein B/ApoB was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human Apolipoprotein B/ApoB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3556) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm and plasma membrane of hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western View Larger

Detection of Human Apolipoprotein B/ApoB by Simple WesternTM. Simple Western shows lysates of human plasma, loaded at 1:50. A specific band was detected for Apolipoprotein B/ApoB at approximately 312 kDa (as indicated) using 5 µg/mL of Goat Anti-Human Apolipoprotein B/ApoB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3556). This experiment was conducted under reducing conditions and using the 66-440kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Apolipoprotein B/ApoB

ApoB is a component of LDL and VLDL particles and chylomicans. ApoB100, the full-length ApoB synthesized in the liver, is a 512 kDa secreted palmetoylated glycoprotein that contains 4536 amino acid residues. A truncated ApoB designated ApoB48, containing the N-terminal 48% of ApoB100, is also produced by the intestine as a result of post-transcriptional modification of the ApoB mRNA. Additionally, C-terminal truncated ApoB, varying in length from ApoB25 to ApoB89, exists as a result of mutations in the ApoB gene. Within the region used as the immunogen, the amino acid sequence of the human peptide is 80% and 82% identical to the mouse and canine sequence, respectively.

Entrez Gene IDs
338 (Human); 238055 (Mouse); 54225 (Rat)
Alternate Names
Apo B-100; APOB; ApoB-100; ApoB-48; apolipoprotein B (including Ag(x) antigen); Apolipoprotein B; apolipoprotein B-100; apolipoprotein B48; FLDB; LDLCQ4; mutant Apo B 100

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Citations for Human Apolipoprotein B/ApoB Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Cytokine-Laden Extracellular Vesicles Predict Patient Prognosis after Cerebrovascular Accident
    Authors: Anthony Fringuello, Philip D. Tatman, Tadeusz Wroblewski, John A. Thompson, Xiaoli Yu, Kevin O. Lillehei et al.
    International Journal of Molecular Sciences
  2. Very-Low-Density Lipoprotein (VLDL)-Producing and Hepatitis C Virus-Replicating HepG2 Cells Secrete No More Lipoviroparticles than VLDL-Deficient Huh7.5 Cells
    Authors: Baptiste Jammart, Maud Michelet, Eve-Isabelle Pécheur, Romain Parent, Birke Bartosch, Fabien Zoulim et al.
    Journal of Virology
  3. Amino acid starvation‐induced LDLR trafficking accelerates lipoprotein endocytosis and LDL clearance
    Authors: Ye Chen, Xiao Wu, Jing Zhang, Guopin Pan, Xiaoyun Wang, Xiaosun Guo et al.
    EMBO reports
  4. Amino acid starvation‐induced LDLR trafficking accelerates lipoprotein endocytosis and LDL clearance
    Authors: Ye Chen, Xiao Wu, Jing Zhang, Guopin Pan, Xiaoyun Wang, Xiaosun Guo et al.
    EMBO reports
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Filter-Dense Multicolor Microscopy.
    Authors: Kijani S, Yrlid U, Heyden M, Levin M, Boren J, Fogelstrand P
    PLoS ONE, 2015-03-04;10(3):e0119499.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Scavenger receptor-BI is a receptor for lipoprotein(a).
    Authors: Yang X, Amar M, Vaisman B, Bocharov A, Vishnyakova T, Freeman L, Kurlander R, Patterson A, Becker L, Remaley A
    J Lipid Res, 2013-06-29;54(9):2450-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  7. Apolipoprotein B-dependent hepatitis C virus secretion is inhibited by the grapefruit flavonoid naringenin.
    Authors: Nahmias Y, Goldwasser J, Casali M, van Poll D, Wakita T, Chung R, Yarmush M
    Hepatology, 2008-05-01;47(5):1437-45.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Co-Immunoprecipitation

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