ApoE is a 34 kDa protein component of serum chylomicrons, VLDL, and HDL particles. It mediates the binding, uptake, and catabolism of these particles through interactions with the ApoE receptor and LDL receptors in the liver and brain. ApoE is important in fatty acid homeostasis and memory formation. Polymorphisms encode three variants (ApoE2, 3, 4) which are differentially related to the development of atherosclerosis and neurogenerative disorders. Mature human ApoE shares 71% amino acid sequence identity with mouse and rat ApoE.
Human Apolipoprotein E/ApoE Antibody
R&D Systems | Catalog # MAB41441
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Western Blot, Neutralization, Immunoprecipitation, ELISA Capture, ELISA Development (Capture)
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 395004
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Product Specifications
Immunogen
E. coli-derived recombinant human ApoE
Lys19-His317
Accession # P02649
Lys19-His317
Accession # P02649
Specificity
Detects human ApoE in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) ApoA1 or rhApoA2 is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Human Apolipoprotein E/ApoE Antibody
Detection of Human Apolipoprotein E/ApoE by Western Blot.
Western blot shows lysates of human lung tissue and human ovary tissue. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Human Apolipoprotein E/ApoE Monoclonal Antibody (Catalog # MAB41441) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Apolipoprotein E/ApoE at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Apolipoprotein E/ApoE Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: Human lung tissue and human ovary tissue
Sample: Human lung tissue and human ovary tissue
Reviewed Applications
Read 1 review rated 4 using MAB41441 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Apolipoprotein E/ApoE
Alternate Names
APOE
Gene Symbol
APOE
UniProt
Additional Apolipoprotein E/ApoE Products
Product Documents for Human Apolipoprotein E/ApoE Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Apolipoprotein E/ApoE Antibody
For research use only
Related Research Areas
Citations for Human Apolipoprotein E/ApoE Antibody
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Customer Reviews for Human Apolipoprotein E/ApoE Antibody (1)
4 out of 5
1 Customer Rating
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Application: Western BlotSample Tested: Human fibroblast and neural stem cellsSpecies: HumanVerified Customer | Posted 02/22/2017Protocol was followed as recommended in the datasheet (Using Immunoblot Buffer Group 1, PVDF) A band around 36 kDa was observed in the cells of interest (iPS-differentiated astrocytes) and none in iPS-differentiated neural stem cells and neurons. A nonspecific band (?) was also observed at 55kDa.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
