Detection of Human Apolipoprotein E/ApoE by Western Blot.
Western blot shows lysates of human lung tissue and human ovary tissue. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Human Apolipoprotein E/ApoE Monoclonal Antibody (Catalog # MAB41441) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Apolipoprotein E/ApoE at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Apolipoprotein E/ApoE
ApoE is a 34 kDa protein component of serum chylomicrons, VLDL, and HDL particles. It mediates the binding, uptake, and catabolism of these particles through interactions with the ApoE receptor and LDL receptors in the liver and brain. ApoE is important in fatty acid homeostasis and memory formation. Polymorphisms encode three variants (ApoE2, 3, 4) which are differentially related to the development of atherosclerosis and neurogenerative disorders. Mature human ApoE shares 71% amino acid sequence identity with mouse and rat ApoE.
Entrez Gene IDs:
348 (Human); 11816 (Mouse); 25728 (Rat)
APOE; Apo-E; Apolipoprotein E; apolipoprotein E3; late onset); MGC1571
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Western Blot: Human Apolipoprotein E/ApoE Antibody [MAB41441].
Human fibroblast and neural stem cells
Other Experimental Details
Other Experimental Details
Protocol was followed as recommended in the datasheet (Using Immunoblot Buffer Group 1, PVDF) A band around 36 kDa was observed in the cells of interest (iPS-differentiated astrocytes) and none in iPS-differentiated neural stem cells and neurons. A nonspecific band (?) was also observed at 55kDa.