Human Apolipoprotein E/ApoE Antibody

Catalog # Availability Size / Price Qty
MAB41441
MAB41441-SP
Detection of Human Apolipoprotein E/ApoE by Western Blot.
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Product Details
Citations (2)
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Reviews (1)

Human Apolipoprotein E/ApoE Antibody Summary

Species Reactivity
Human
Specificity
Detects human ApoE in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) ApoA1 or rhApoA2 is observed.
Source
Monoclonal Rat IgG2A Clone # 395004
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human ApoE
Lys19-His317
Accession # P02649
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human Apolipoprotein E/ApoE by Western Blot. View Larger

Detection of Human Apolipoprotein E/ApoE by Western Blot. Western blot shows lysates of human lung tissue and human ovary tissue. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Human Apolipoprotein E/ApoE Monoclonal Antibody (Catalog # MAB41441) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Apolipoprotein E/ApoE at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Apolipoprotein E/ApoE

ApoE is a 34 kDa protein component of serum chylomicrons, VLDL, and HDL particles. It mediates the binding, uptake, and catabolism of these particles through interactions with the ApoE receptor and LDL receptors in the liver and brain. ApoE is important in fatty acid homeostasis and memory formation. Polymorphisms encode three variants (ApoE2, 3, 4) which are differentially related to the development of atherosclerosis and neurogenerative disorders. Mature human ApoE shares 71% amino acid sequence identity with mouse and rat ApoE.

Entrez Gene IDs
348 (Human); 11816 (Mouse); 25728 (Rat)
Alternate Names
APOE; Apo-E; Apolipoprotein E; apolipoprotein E3; late onset); MGC1571

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Citations for Human Apolipoprotein E/ApoE Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Serum Apolipoprotein E and Other Inflammatory Markers Can Identify Non-Responding Patients to a Dendritic Cell Vaccine
    Authors: H Leeman, E Kaminska, D Green, M Bodman-Smi, A Gravett, K Bodman-Smi, J Copier, G Coulton, A Fusi, AG Dalgleish
    Transl Oncol, 2019;12(3):397-403.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development (Capture)
  2. Effect of human very low-density lipoproteins on cardiotrophin-like cytokine factor 1 (CLCF1) activity
    Authors: S Pasquin, S Chehboun, A Dejda, Y Meliani, V Savin, GJ Warner, R Bosse, A Tormo, G Mayer, M Sharma, P Sapieha, C Martel, JF Gauchat
    Sci Rep, 2018;8(1):3990.
    Applications: Neutralization

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human Apolipoprotein E/ApoE Antibody

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Human Apolipoprotein E/ApoE Antibody
By Anonymous on 02/22/2017
Application: WB Sample Tested: Human fibroblast and neural stem cells Species: Human

Protocol was followed as recommended in the datasheet (Using Immunoblot Buffer Group 1, PVDF) A band around 36 kDa was observed in the cells of interest (iPS-differentiated astrocytes) and none in iPS-differentiated neural stem cells and neurons. A nonspecific band (?) was also observed at 55kDa.