Human Apolipoprotein E/ApoE Antibody

  (1 citations)
(1 Review)
    
Datasheet / CoA / SDS
Product Details
Citations (1)
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  • Species Reactivity
    Human
  • Specificity
    Detects human ApoE in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) ApoA1 or rhApoA2 is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 395004
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human ApoE
    Lys19-His317
    Accession # P02649
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Product Datasheets

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Note: Certificate of Analysis not available for kit components.

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of Human Apolipoprotein E/ApoE by Western Blot. Western blot shows lysates of human lung tissue and human ovary tissue. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Human Apolipoprotein E/ApoE Monoclonal Antibody (Catalog # MAB41441) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Apolipoprotein E/ApoE at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Apolipoprotein E/ApoE

ApoE is a 34 kDa protein component of serum chylomicrons, VLDL, and HDL particles. It mediates the binding, uptake, and catabolism of these particles through interactions with the ApoE receptor and LDL receptors in the liver and brain. ApoE is important in fatty acid homeostasis and memory formation. Polymorphisms encode three variants (ApoE2, 3, 4) which are differentially related to the development of atherosclerosis and neurogenerative disorders. Mature human ApoE shares 71% amino acid sequence identity with mouse and rat ApoE.

  • Entrez Gene IDs:
    348 (Human); 11816 (Mouse); 25728 (Rat)
  • Alternate Names:
    APOE; Apo-E; Apolipoprotein E; apolipoprotein E3; late onset); MGC1571
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Effect of human very low-density lipoproteins on cardiotrophin-like cytokine factor 1 (CLCF1) activity
    Authors: S Pasquin, S Chehboun, A Dejda, Y Meliani, V Savin, GJ Warner, R Bosse, A Tormo, G Mayer, M Sharma, P Sapieha, C Martel, JF Gauchat
    Sci Rep, 2018;8(1):3990.
    Application: Neut

FAQs

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Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A Isotype Control

Ctrl MAB006 96  
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Normal Rat IgG Control

Ctrl 6-001-A 11
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Normal Rat IgG Control (Azide Free)

Ctrl 6-001-F 1
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Rat IgG2A Isotype Control

Ctrl MAB006R  
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Reconstitution Buffers
Description Application Cat# Citations Images  

Reconstitution Buffer 1 (PBS)

RB01
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Secondary Antibodies
Description Application Cat# Citations Images  

Rat IgG HRP-conjugated Antibody

WB , Simple Western HAF005 7  
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Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

Flow F0113 2  
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Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody

Flow , IHC , ICC NL013 2
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Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0105B 3  
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Goat Anti-Rat IgG Antibody

WB AF005 1
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Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 2
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Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow , IHC , ICC NL015 1
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Rat IgG VisUCyte HRP Polymer Antibody

IHC VC005  
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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow , IHC , ICC NL014
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0104B  
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Average Rating: 4 (Based on 1 review)

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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Western Blot.

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Images Ratings Applications Species Reviewed By Date Details
Western Blot Human Apolipoprotein E/ApoE Antibody MAB41441 Array MAB41441
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Very Good
 WB Human Anonymous 02/22/2017
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Image Details
Western Blot Human Apolipoprotein E/ApoE Antibody MAB41441
Western Blot: Human Apolipoprotein E/ApoE Antibody [MAB41441].

Summary

Application Western Blot
Sample Tested Human fibroblast and neural stem cells
Species Human

Other Experimental Details

Other Experimental Details Protocol was followed as recommended in the datasheet (Using Immunoblot Buffer Group 1, PVDF) A band around 36 kDa was observed in the cells of interest (iPS-differentiated astrocytes) and none in iPS-differentiated neural stem cells and neurons. A nonspecific band (?) was also observed at 55kDa.

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