Human Apolipoprotein E/ApoE Antibody (395004)

R&D Systems | Catalog # MAB41441

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot, Immunoprecipitation, ELISA Capture, ELISA Development (Capture)

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 395004
View all Apolipoprotein E/ApoE Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human ApoE
Lys19-His317
Accession # P02649

Specificity

Detects human ApoE in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) ApoA1 or rhApoA2 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Human Apolipoprotein E/ApoE Antibody (395004)

Detection of Human Apolipoprotein E/ApoE antibody by Western Blot.

Detection of Human Apolipoprotein E/ApoE by Western Blot.

Western blot shows lysates of human lung tissue and human ovary tissue. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Human Apolipoprotein E/ApoE Monoclonal Antibody (Catalog # MAB41441) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Apolipoprotein E/ApoE at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Apolipoprotein E/ApoE Antibody (395004)

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: Human lung tissue and human ovary tissue

Reviewed Applications

Read 1 review rated 4 using MAB41441 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Apolipoprotein E/ApoE

ApoE is a 34 kDa protein component of serum chylomicrons, VLDL, and HDL particles. It mediates the binding, uptake, and catabolism of these particles through interactions with the ApoE receptor and LDL receptors in the liver and brain. ApoE is important in fatty acid homeostasis and memory formation. Polymorphisms encode three variants (ApoE2, 3, 4) which are differentially related to the development of atherosclerosis and neurogenerative disorders. Mature human ApoE shares 71% amino acid sequence identity with mouse and rat ApoE.

Alternate Names

APOE

Entrez Gene IDs

348 (Human); 11816 (Mouse); 25728 (Rat)

Gene Symbol

APOE

UniProt

P02649

Additional Apolipoprotein E/ApoE Products

Product Documents for Human Apolipoprotein E/ApoE Antibody (395004)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Apolipoprotein E/ApoE Antibody (395004)

For research use only

Citations for Human Apolipoprotein E/ApoE Antibody (395004)

Customer Reviews for Human Apolipoprotein E/ApoE Antibody (395004) (1)

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  • Human Apolipoprotein E/ApoE Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Human fibroblast and neural stem cells
    Species: Human
    Verified Customer | Posted 02/22/2017
    Protocol was followed as recommended in the datasheet (Using Immunoblot Buffer Group 1, PVDF) A band around 36 kDa was observed in the cells of interest (iPS-differentiated astrocytes) and none in iPS-differentiated neural stem cells and neurons. A nonspecific band (?) was also observed at 55kDa.
    Human Apolipoprotein E/ApoE Antibody (395004) MAB41441

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Associated Pathways

A-beta Pathways: Uptake & Degradation
A-beta Pathways: Uptake & Degradation Thumbnail