Human Apolipoprotein E3/ApoE3 Antibody

Catalog # Availability Size / Price Qty
MAB41443-100
MAB41443-SP
Apolipoprotein E3/ApoE3  in Human Brain.
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Human Apolipoprotein E3/ApoE3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Apolipoprotein E3/ApoE3 in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 960318
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human Apolipoprotein E3/ApoE3 sythetic peptide
Accession # P02649
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
5-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry Apolipoprotein E3/ApoE3  in Human Brain. View Larger

Apolipoprotein E3/ApoE3 in Human Brain. Apolipoprotein E3/ApoE3 was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Mouse Anti-Human Apolipoprotein E3/ApoE3 Monoclonal Antibody (Catalog # MAB41443) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Apolipoprotein E3/ApoE3

ApoE is a major protein component of serum LDL, VLDL, HDL, and chylomicrons. It is produced predominantly by hepatocytes, macrophages, and non-neuronal cells in the CNS. ApoE-containing particles transport triglycerides and cholesterol to peripheral tissues for cellular uptake and catabolism (1‑4). Mature human ApoE is a 37 kDa glycoprotein that consists of an N-terminal domain composed of four bundled alpha -helices, plus a hinge region and an extended alpha -helical C-terminal domain (2, 5). Its amphipathic nature and flexible structure enables it to adopt dramatically different conformations upon lipid association (2). ApoE is monomeric in lipid particles, although it forms oligomers when lipid-free (6). ApoE3 is the most abundant of the three common alleles in human; ApoE2 and ApoE4 differ by single aa substitutions (1). Mature human ApoE shares 71% aa sequence identity with mouse and rat ApoE. LDL receptor family proteins preferentially bind and internalize the lipid-bound form of ApoE with the exception of VLDLR which also efficiently internalizes lipid-free ApoE (7, 8). Lipoprotein uptake is facilitated by the initial binding of ApoE to cell surface heparan sulfate proteoglycans (HSPG) (9). Receptor/HSPG binding and lipid interactions primarily involve the N- and C-terminal regions of ApoE, respectively (2). Recycled lipid-free ApoE is formed into HDL particles through interactions with the lipid transporter ABCA1 (10). High cellular sterol content activates the nuclear hormone receptor LXR which promotes increased ApoE synthesis and increased sterol efflux, while low sterol content induces LDL R expression with increased sterol uptake and decreased ApoE production (11). ApoE3 dampens the TNF-alpha induced inflammatory response in vascular endothelial cells (12). In the CNS, ApoE blocks production of the amyloid A beta peptide by inhibiting the gamma -secretase cleavage of APP (13). It also complexes with A beta and promotes A beta internalization via LRP2 (14, 15).

References
  1. Martins, I.J. et al. (2006) Mol. Pschiatry 11:721.
  2. Hatters, D.M. et al. (2006) Trends Biochem. Sci. 31:445.
  3. Heeren, J. et al. (2006) Arterioscler. Thromb. Vasc. Biol. 26:442.
  4. Mahley, R.W. et al. (1984) J. Lipid. Res. 25:1277.
  5. Zannis, V.I. et al. (1984) J. Biol. Chem. 259:5495.
  6. Perugini, M.A. et al. (2000) J. Biol. Chem. 275:36758.
  7. Ruiz, J. et al. (2005) J. Lipid Res. 46:1721.
  8. Chroni, A. et al. (2005) Biochemistry 44:13132.
  9. Futamura, M. et al. (2005) J. Biol. Chem. 280:5414.
  10. Krimbou, L. et al. (2004) J. Lipid. Res. 45:839.
  11. Lucic, D. et al. (2007) J. Lipid Res. 48:366.
  12. Mullick, A.E. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:339.
  13. Irizarry, M.C. et al. (2004) J. Neurochem. 90:1132.
  14. Naslund, J. et al. (1995) Neuron 15:219.
  15. Zerbinatti, C.V. et al. (2006) J. Biol. Chem. 281:36180.
Entrez Gene IDs
348 (Human); 11816 (Mouse)
Alternate Names
AD2; Apo-E; ApoE4; apolipoprotein E; Apolipoprotein E3; LDLCQ5; LPG

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Mouse IgG1 Isotype Control

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Reconstitution Buffers

Secondary Antibodies

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