Detection of Human Apolipoprotein M/ApoM by Western Blot.
Western blot shows lysates of human plasma and liver tissue. PVDF membrane was probed with 0.5 µg/mL of Human Apolipoprotein M/ApoM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4550) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Apolipoprotein M/ApoM at approximately 25 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Apolipoprotein M/ApoM
Apolipoprotein M (ApoM; also known as Protein G3a) is a 25 kDa, secreted member of the lipocalin family of proteins. It is produced by hepatocytes and renal tubular epithelium, and represents a minor component of HDL particles. Human ApoM is 188 amino acids (aa) in length. It contains an uncleaved, hydrophobic N-terminal segment and one utilized N-linked glycosylation site (mouse ApoM shows no glycosylation). At least two potential isoforms exist. One shows an alternate start site at Met73, while a second shows the same start site plus a 21 aa substitution for aa 181‑188. Human ApoM shares 91% and 80% aa identity with pig and mouse ApoM, respectively.
Entrez Gene IDs:
55937 (Human); 55938 (Mouse); 55939 (Rat)
Apolipoprotein M; APOM; apo-M; G3A; HSPC336; MGC22400; NG20; NG20-like protein; Protein G3a
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