Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human ASAHL/N‑acylethanolamine-hydrolyzing Acid A isoform 1
Ser29-Lys359 (Val151Ile)
Accession # Q02083

Specificity

Detects human ASAHL/N‑acylethanolamine-hydrolyzing Acid A in direct ELISAs and Western blots. In Western blots, approximately 25% cross‑reactivity with recombinant mouse ASAHL is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human ASAHL/N‑acylethanolamine-hydrolyzing Acid A Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human N-acylethanolamine-hydrolyzing Acid Amidase/ASAHL (Catalog # 4494-AH), see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Human N-acylethanolamine-hydrolyzing Acid Amidase/ASAHL (Catalog # 4494‑AH)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase

ASAHL (N-acylsphingosine-amidohydrolase-like; N acylethanolamine hydrolyzine acid amidase; also acid ceramidase like protein) is a 30 kDa member of the acid ceramidase family. It is a secreted lysosomal glycoprotein that cleaves fatty acid amides such as palmitoylethanolamine. Human ASAHL is synthesized as a 359 amino acid (aa) preproprecursor that contains a 28 aa signal sequence, a 96 aa prosegment, and a 30 31 kDa, 235 aa mature region that begins at Cys125. Multiple isoforms likely exist. One isoform shows a deletion of aa 324 359, while another has a two aa substitution for aa 198 359. The human proform (aa 29 359) is 78% aa identical to mouse ASAHL proform.

References

    Alternate Names

    Acid ceramidase-like protein, ASAH-like protein, NAAA, Nacylethanolaminehydrolyzing Acid Amidase

    Entrez Gene IDs

    27163 (Human); 67111 (Mouse); 497009 (Rat)

    Gene Symbol

    NAAA

    UniProt

    Q02083

    Additional ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase Products

    Product Documents for Human ASAHL/N‑acylethanolamine-hydrolyzing Acid A Antibody

    Certificate of Analysis

    To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

    Note: Certificate of Analysis not available for kit components.

    Download SDS

    Product Specific Notices for Human ASAHL/N‑acylethanolamine-hydrolyzing Acid A Antibody

    For research use only

    Citations for Human ASAHL/N‑acylethanolamine-hydrolyzing Acid A Antibody

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