Detection of Human ATG4A by Western Blot.
Western blot shows lysates of HUVEC human umbilical vein endothelial cells, MCF‑7 human breast cancer cell line, and human skeletal muscle tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human ATG4A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4324) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ATG4A at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
ATG4A (Autophagy related protein 4 homolog A; also Autophagin 2) is a 50 kDa cytosolic cysteine protease mostly found in skeletal muscle and brain. It belongs to the Autophagin protein family which is involved in autophagy, a biological process in which endogenous protein and damaged organelles are destroyed. There is a potential for multiple splice forms, namely isoform 1, isoform 2, and isoform 3. Human ATG4A shares 92% and 94% aa sequence identity with mouse and canine ATG4A, respectively.
ATG4 Autophagy Related 4 Homolog A
Entrez Gene IDs:
115201 (Human); 666468 (Mouse)
APG4 autophagy 4 homolog A (S. cerevisiae); Apg4a; ATG4 autophagy related 4 homolog A (S. cerevisiae); ATG4A; Atg4al; Autl2; AUT-like 2 cysteine endopeptidase; AUT-like 2, cysteine endopeptidase (S. cerevisiae); Autophagin 2; autophagin-2; cysteine endopeptidase; cysteine protease ATG4A; EC 3.4.22; EC 3.4.22.-; hAPG4A
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