Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 391017
View all BACE-2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human BACE-2
Ala21-Tyr473
Accession # Q9Y5Z0

Specificity

Detects human BACE‑2 Ectodomain in direct ELISAs. In direct ELISAs, less than 10% cross-reactivity with recombinant mouse BACE-2 is observed and no cross-reactivity with recombinant human (rh) BACE, rhCathepsin D, or rhCathepsin E is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Human BACE‑2 Ectodomain Antibody

BACE-2 antibody in Human Skin by Immunohistochemistry (IHC-P).

BACE‑2 in Human Skin.

BACE-2 was detected in immersion fixed paraffin-embedded sections of human skin using Rat Anti-Human BACE-2 Ectodomain Monoclonal Antibody (Catalog # MAB4097) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human BACE‑2 Ectodomain Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human skin, placenta, and prostate

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: BACE-2

BACE-2 ( beta -site APP cleaving enzyme 2; also Asp-1 and Memapsin-1) is a 73‑75 kDa member of the peptidase A1 family. It is expressed both in- and outside the CNS, and is classified as a Golgi aspartyl protease. Its status as a beta -secretase is unclear; if it has physiological activity, it may be associated with glia. Mature BACE-2 is a 456 amino acid (aa) type I transmembrane glycoprotein. It contains a 411 aa extracellular domain (aa 63‑473) that has two catalytic residues (Asp93 and Asp289). Two potential splice forms exist; one shows a deletion of aa 329‑378, while a second shows an 18 aa substitution for aa 380‑518. Over aa 63‑466, human BACE-2 shares 92% aa sequence identity with mouse BACE-2.

Long Name

beta-site Ayloid Precursor Protein Cleaving Enzyme 2

Alternate Names

BACE2

Entrez Gene IDs

25825 (Human); 56175 (Mouse); 288227 (Rat)

Gene Symbol

BACE2

UniProt

Q9Y5Z0

Additional BACE-2 Products

Product Documents for Human BACE‑2 Ectodomain Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human BACE‑2 Ectodomain Antibody

For research use only

Related Research Areas

Aspartic Proteases and Regulators

Customer Reviews for Human BACE‑2 Ectodomain Antibody

There are currently no reviews for this product. Be the first to review Human BACE‑2 Ectodomain Antibody and earn rewards!

Have you used Human BACE‑2 Ectodomain Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies