Detects human BACE‑2 Ectodomain in direct ELISAs. In direct ELISAs, less than 10% cross-reactivity with recombinant mouse BACE-2 is observed and no cross-reactivity with recombinant human (rh) BACE, rhCathepsin D, or rhCathepsin E is observed.
Monoclonal Rat IgG2A Clone # 391017
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human BACE-2 Ala21-Tyr473 Accession # Q9Y5Z0
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
BACE‑2 in Human Skin. BACE‑2 was detected in immersion fixed paraffin-embedded sections of human skin using Rat Anti-Human BACE‑2 Ectodomain Monoclonal Antibody (Catalog # MAB4097) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
BACE-2 ( beta -site APP cleaving enzyme 2; also Asp-1 and Memapsin-1) is a 73‑75 kDa member of the peptidase A1 family. It is expressed both in- and outside the CNS, and is classified as a Golgi aspartyl protease. Its status as a beta -secretase is unclear; if it has physiological activity, it may be associated with glia. Mature BACE-2 is a 456 amino acid (aa) type I transmembrane glycoprotein. It contains a 411 aa extracellular domain (aa 63‑473) that has two catalytic residues (Asp93 and Asp289). Two potential splice forms exist; one shows a deletion of aa 329‑378, while a second shows an 18 aa substitution for aa 380‑518. Over aa 63‑466, human BACE-2 shares 92% aa sequence identity with mouse BACE-2.
beta-site Ayloid Precursor Protein Cleaving Enzyme 2
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.