Human BACE-2 Ectodomain Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human BACE‑2 Ectodomain in direct ELISAs. In direct ELISAs, less than 10% cross-reactivity with recombinant mouse BACE-2 is observed and no cross-reactivity with recombinant human (rh) BACE, rhCathepsin D, or rhCathepsin E is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 391017
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human BACE-2
    Ala21-Tyr473
    Accession # Q9Y5Z0
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunohistochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
BACE‑2 in Human Skin. BACE‑2 was detected in immersion fixed paraffin-embedded sections of human skin using Rat Anti-Human BACE‑2 Ectodomain Monoclonal Antibody (Catalog # MAB4097) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BACE-2
BACE-2 ( beta -site APP cleaving enzyme 2; also Asp-1 and Memapsin-1) is a 73‑75 kDa member of the peptidase A1 family. It is expressed both in- and outside the CNS, and is classified as a Golgi aspartyl protease. Its status as a beta -secretase is unclear; if it has physiological activity, it may be associated with glia. Mature BACE-2 is a 456 amino acid (aa) type I transmembrane glycoprotein. It contains a 411 aa extracellular domain (aa 63‑473) that has two catalytic residues (Asp93 and Asp289). Two potential splice forms exist; one shows a deletion of aa 329‑378, while a second shows an 18 aa substitution for aa 380‑518. Over aa 63‑466, human BACE-2 shares 92% aa sequence identity with mouse BACE-2.
  • Long Name:
    beta-site Ayloid Precursor Protein Cleaving Enzyme 2
  • Entrez Gene IDs:
    25825 (Human); 56175 (Mouse); 288227 (Rat)
  • Alternate Names:
    AEPLC; Asp 1; ASP1; ASP21; Aspartic-like protease 56 kDa; Aspartyl protease 1; BACE2; BACE-2; beta secretase 2; beta-secretase 2; beta-site amyloid beta A4 precursor protein-cleaving enzyme 2; Beta-site amyloid precursor protein cleaving enzyme 2; Beta-site APP cleaving enzyme 2; beta-site APP-cleaving enzyme 2; beta-site APP-cleaving enzyme 2, EC 3.4.2310ALP5656 kDa aspartic-like protease; CEAP1; Down region aspartic protease; Down syndrome region aspartic protease; DRAPBAE2; EC 3.4.23; EC 3.4.23.45; memapsin-1; Membrane-associated aspartic protease 1; theta-secretase; transmembrane aspartic proteinase Asp1
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