Human BAFF/BLyS/TNFSF13B Quantikine ELISA Kit

Newer Version Available: DBLYS0B

Discontinued Product

DBLYS0 has been discontinued and is replaced by DBLYS0B.

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Citations (47)
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Human BAFF/BLyS/TNFSF13B Quantikine ELISA Kit Summary

Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (75 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity
11.9 pg/mL
Assay Range
31.2 - 2,000 pg/mL (Cell Culture Supernates), 62.5 - 4,000 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human BAFF
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.

Product Summary

The Quantikine Human BAFF Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human BAFF in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human BAFF and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human BAFF showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human BAFF.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 141 462 871 156 482 955
Standard Deviation 7.53 18.8 37.5 13.5 41.7 96.7
CV% 5.3 4.1 4.3 8.7 8.7 10.1

Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 458 1371 2545 498 1476 2854
Standard Deviation 17.5 82.8 123 38.9 106 256
CV% 3.8 6 4.8 7.8 7.2 9

Recovery

The recovery of BAFF spiked to levels throughout the range of the assay in various matrices was evaluated. One sample showed < 50% recovery and was not included in the data.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 101 87-112
EDTA Plasma (n=4) 101 85-114
Heparin Plasma (n=4) 98 86-110
Serum (n=4) 101 86-114

Linearity

To assess the linearity of the assay, samples spiked with high concentrations of BAFF were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Product Datasheets

Preparation and Storage

Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: BAFF/BLyS/TNFSF13B

BAFF (B-cell activating factor), also known as BLyS, TALL-1, THANK, and TNFSF13B, is a TNF superfamily ligand that plays a critical role in the development and survival of B lineage cells. It can be expressed as a homotrimer or as a heteromer in association with the related protein APRIL. It is produced by many hematopoietic cell types, and a soluble form can be released by activated neutrophils. Both BAFF and APRIL signal through the receptors BCMA and TACI, and BAFF additionally signals through BAFF R. Mice that overexpress BAFF exhibit elevated B cell numbers, increased formation and size of germinal centers, and symptoms of autoimmunity. In addition, BAFF costimulates T cell activation, promotes a Th1 biased immune response, and promotes the expansion of Treg cells.

Long Name:
B cell Activating Factor
Entrez Gene IDs:
10673 (Human); 24099 (Mouse); 498666 (Rat)
Alternate Names:
ApoL related ligand TALL-1; B lymphocyte stimulator; BAFF; BAFFB-cell activating factor; B-cell-activating factor; BLyS; BLYSB-lymphocyte stimulator; CD257 antigen; CD257; Dendritic cell-derived TNF-like molecule; DTL; TALL1; TALL-1delta BAFF; TALL1Delta4 BAFF; THANK; TNF- and APOL-related leukocyte expressed ligand 1; TNF and ApoL-related leukocyte expressed ligand 1; TNF homolog that activates apoptosis; TNFSF13B; TNFSF20; tumor necrosis factor (ligand) superfamily, member 13b; tumor necrosis factor (ligand) superfamily, member 20; tumor necrosis factor ligand superfamily member 13B; tumor necrosis factor superfamily, member 13b; tumor necrosis factor-like protein ZTNF4; ZTNF4

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 3 hours on a horizontal orbital microplate shaker.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 1 hour on the shaker.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human BAFF/BLyS/TNFSF13B Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

47 Citations: Showing 1 - 10
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  1. Peripheral B-Cell Subset Distribution in Primary Antiphospholipid Syndrome
    Authors: L Alvarez, L Riancho, J Calvo-Alén, M Lopez Hoyo, V Martinez-T
    Int J Mol Sci, 2018;19(2):.
    Species: Human
    Sample Types: Serum
  2. Testosterone is an endogenous regulator of BAFF and splenic B cell number
    Authors: AS Wilhelmson, M Lantero Ro, A Stubelius, P Fogelstran, I Johansson, MB Buechler, S Lianoglou, VN Kapoor, ME Johansson, JB Fagman, A Duhlin, P Tripathi, A Camponesch, BT Porse, AG Rolink, H Nissbrandt, SJ Turley, H Carlsten, IL Mårtensson, MCI Karlsson, Å Tivesten
    Nat Commun, 2018;9(1):2067.
    Species: Human
    Sample Types: Serum
  3. Comparison of non-invasive biomarkers faecal BAFF, calprotectin and FOBT in discriminating IBS from IBD and evaluation of intestinal inflammation
    Authors: Y Fu, L Wang, C Xie, K Zou, L Tu, W Yan, X Hou
    Sci Rep, 2017;7(1):2669.
    Species: Human
    Sample Types: Faecal
  4. BAFF Index and CXCL13 levels in the cerebrospinal fluid associate respectively with intrathecal IgG synthesis and cortical atrophy in multiple sclerosis at clinical onset
    Authors: M Puthenpara, L Federle, S Miante, A Zito, E Toffanin, S Ruggero, M Ermani, S Pravato, D Poggiali, P Perini, F Rinaldi, P Gallo
    J Neuroinflammation, 2017;14(1):11.
    Species: Human
    Sample Types: Serum
  5. Intrathecal Th17- and B cell-associated cytokine and chemokine responses in relation to clinical outcome in Lyme neuroborreliosis: a large retrospective study
    Authors: P Gyllemark, P Forsberg, J Ernerudh, AJ Henningsso
    J Neuroinflammation, 2017;14(1):27.
    Species: Human
    Sample Types: Serum
  6. Elevated C-X-C motif ligand 13 and B-cell-activating factor levels in neuromyelitis optica during remission
    Authors: S Wang, T Yang, J Wan, Y Zhang, Y Fan
    Brain Behav, 2017;7(4):e00648.
    Species: Human
    Sample Types: Serum
  7. Case-control, exploratory study of cerebrospinal fluid chemokines/cytokines and lymphocyte subsets in childhood Tourette syndrome with positive streptococcal markers
    Authors: MR Pranzatell, ED Tate, TJ Allison
    Cytokine, 2017;96(0):49-53.
    Species: Human
    Sample Types: CSF
  8. Effect of FCGR polymorphism on the occurrence of late-onset neutropenia and flare-free survival in rheumatic patients treated with rituximab
    Authors: S Ajeganova, D Tesfa, H Hägglund, B Fadeel, I Vedin, AL Zignego, J Palmblad
    Arthritis Res. Ther, 2017;19(1):44.
    Species: Human
    Sample Types: Serum
  9. Smoking and pre-existing organ damage reduce the efficacy of belimumab in systemic lupus erythematosus
    Authors: I Parodis, C Sjöwall, A Jönsen, D Ramsköld, A Zickert, M Frodlund, A Sohrabian, L Arnaud, J Rönnelid, V Malmström, AA Bengtsson, I Gunnarsson
    Autoimmun Rev, 2017;0(0):.
    Species: Human
    Sample Types: Serum
  10. A distinct plasmablast and na�ve B-cell phenotype in primary immune thrombocytopenia
    Haematologica, 2016;101(6):698-706.
    Species: Human
    Sample Types: Serum
  11. Hepatitis B Virus e Antigen Regulates Monocyte Function and Promotes B Lymphocyte Activation
    Authors: Bingru Lu
    Viral Immunol, 2016;0(0):.
  12. Plasmacytoid dendritic cells and myeloid cells differently contribute to BAFF over-expression during primary HIV infection.
    Authors: Borhis G, Burelout C, Chaoul N, Smith N, Goujard C, Meyer L, Paul S, Saoudin H, Hosmalin A, Gilbert C, Herbeuval J, Richard Y
    AIDS, 2016;30(0):365-376.
    Species: Human
    Sample Types: Cell Culture Supernates
  13. Human Basophils Modulate Plasma Cell Differentiation and Maturation
    Authors: Almut Meyer-Bahl
    J. Immunol., 2016;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  14. Serum B-cell activating factor predicts prognosis in nasal type, extranodal NK/T cell lymphoma
    Cytokine, 2016;88(0):196-198.
    Species: Human
    Sample Types: Serum
  15. Toll-like receptor ligands induce cytokine and chemokine production in human inner ear endolymphatic sac fibroblasts
    Authors: Shigeharu Fujieda
    Auris Nasus Larynx, 2016;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  16. B Lymphocyte Stimulator (BLyS) is expressed in human adipocytes in vivo and is related to obesity but not to insulin resistance.
    Authors: Muller N, Schulte D, Hillebrand S, Turk K, Hampe J, Schafmayer C, Brosch M, von Schonfels W, Ahrens M, Zeuner R, Schroder J, Bluher M, Gutschow C, Freitag-Wolf S, Stelmach-Mardas M, Saggau C, Schreiber S, Laudes M
    PLoS ONE, 2014;9(4):e94282.
    Species: Human
    Sample Types: Serum
  17. BAFF and BAFF receptor levels correlate with B cell subset activation and redistribution in controlled human malaria infection.
    Authors: Scholzen A, Teirlinck A, Bijker E, Roestenberg M, Hermsen C, Hoffman S, Sauerwein R
    J Immunol, 2014;192(8):3719-29.
    Species: Human
    Sample Types: Plasma
  18. Neutrophil-mediated IFN activation in the bone marrow alters B cell development in human and murine systemic lupus erythematosus.
    Authors: Palanichamy A, Bauer J, Yalavarthi S, Meednu N, Barnard J, Owen T, Cistrone C, Bird A, Rabinovich A, Nevarez S, Knight J, Dedrick R, Rosenberg A, Wei C, Rangel-Moreno J, Liesveld J, Sanz I, Baechler E, Kaplan M, Anolik J
    J Immunol, 2014;192(3):906-18.
    Species: Human
    Sample Types: Cell Culture Supernates
  19. The B lymphocyte differentiation factor (BAFF) is expressed in the airways of children with CF and in lungs of mice infected with Pseudomonas aeruginosa.
    Authors: Neill, Daniel R, Saint, Gemma L, Bricio-Moreno, Laura, Fothergill, Joanne L, Southern, Kevin W, Winstanley, Craig, Christmas, Stephen, Slupsky, Joseph R, McNamara, Paul S, Kadioglu, Aras, Flanagan, Brian F
    PLoS ONE, 2014;9(5):e95892.
    Species: Human
    Sample Types: BALF
  20. Serum levels of B-cell activating factor in chronic hepatitis B virus infection: association with clinical diseases.
    Authors: Yang, Cuiling, Li, Na, Wang, Yawen, Zhang, Pingping, Zhu, Qianqian, Li, Fang, Han, Qunying, Lv, Yi, Yu, Ling, Wei, Ping, Liu, Zhengwen
    J Interferon Cytokine Res, 2014;34(10):787-94.
    Species: Human
    Sample Types: Serum
  21. The strength of the antibody response to the nematode Ascaris lumbricoides inversely correlates with levels of B-Cell Activating Factor (BAFF).
    Authors: Bornacelly, Adriana, Mercado, Dilia, Acevedo, Nathalie, Caraballo, Luis
    BMC Immunol, 2014;15(0):22.
    Species: Human
    Sample Types: Plasma
  22. IFN-beta therapy modulates B-cell and monocyte crosstalk via TLR7 in multiple sclerosis patients.
    Authors: Giacomini E, Severa M, Rizzo F, Mechelli R, Annibali V, Ristori G, Riccieri V, Salvetti M, Coccia E
    Eur J Immunol, 2013;43(7):1963-72.
    Species: Human
    Sample Types: Serum
  23. BAFF/APRIL system in pediatric OMS: relation to severity, neuroinflammation, and immunotherapy.
    Authors: Pranzatelli, Michael, Tate, Elizabet, McGee, Nathan R, Travelstead, Anna L, Colliver, Jerry A, Ness, Jayne M, Ransohoff, Richard
    J Neuroinflammation, 2013;10(0):10.
    Species: Human
    Sample Types: Serum
  24. Study of the quantitative, functional, cytogenetic, and immunoregulatory properties of bone marrow mesenchymal stem cells in patients with B-cell chronic lymphocytic leukemia.
    Authors: Pontikoglou C, Kastrinaki M, Klaus M, Kalpadakis C, Katonis P, Alpantaki K, Pangalis G, Papadaki H
    Stem Cells Dev, 2013;22(9):1329-41.
    Species: Human
    Sample Types: Cell Culture Supernates
  25. Role of BAFF in Opsoclonus-Myoclonus syndrome, a bridge between cancer and autoimmunity.
    Authors: Raffaghello L, Fuhlhuber V, Bianchi G, Conte M, Blaes F, Gambini C, Pistoia V
    J Leukoc Biol, 2013;94(1):183-91.
    Species: Human
    Sample Types: Whole Cells
  26. BAFF levels are increased in lesional skin and sera in patients with cutaneous T-cell lymphoma.
    Authors: Ohmatsu H, Sugaya M, Miyagaki T, Suga H, Fujita H, Asano Y, Tada Y, Kadono T, Sato S
    Br. J. Dermatol., 2012;167(2):359-67.
    Species: Human
    Sample Types: Serum
  27. IL-15 Expression on RA Synovial Fibroblasts Promotes B Cell Survival.
    Authors: Benito-Miguel M, Garcia-Carmona Y, Balsa A, Bautista-Caro MB, Arroyo-Villa I, Cobo-Ibanez T, Bonilla-Hernan MG, De Ayala CP, Sanchez-Mateos P, Martin-Mola E, Miranda-Carus ME
    PLoS ONE, 2012;7(7):e40620.
    Species: Human
    Sample Types: Cell Culture Supernates
  28. Default in plasma and intestinal IgA responses during acute infection by simian immunodeficiency virus.
    Authors: Chaoul N, Burelout C, Peruchon S, van Buu B, Laurent P, Proust A, Raphael M, Garraud O, Le Grand R, Prevot S, Richard Y
    Retrovirology, 2012;9(0):43.
    Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
    Sample Types: Plasma
  29. B-cell activating factor levels in rheumatoid arthritis patients in response to treatment with biologics.
    J. Interferon Cytokine Res., 2012;32(7):338-40.
    Species: Human
    Sample Types: Serum
  30. Active chronic sarcoidosis is characterized by increased transitional blood B cells, increased IL-10-producing regulatory B cells and high BAFF levels.
    Authors: Saussine A, Tazi A, Feuillet S, Rybojad M, Juillard C, Bergeron A, Dessirier V, Bouhidel F, Janin A, Bensussan A, Bagot M, Bouaziz J
    PLoS ONE, 2012;7(8):e43588.
    Species: Human
    Sample Types: Serum
  31. Recovery of B-cell homeostasis after rituximab in chronic graft-versus-host disease.
    Authors: Sarantopoulos S, Stevenson KE, Kim HT
    Blood, 2011;117(7):2275-83.
    Species: Human
    Sample Types: Plasma
  32. Primary B-cell deficiencies reveal a link between human IL-17-producing CD4 T-cell homeostasis and B-cell differentiation.
    Authors: Barbosa RR, Silva SP, Silva SL, Melo AC, Pedro E, Barbosa MP, Pereira-Santos MC, Victorino RM, Sousa AE
    PLoS ONE, 2011;6(8):e22848.
    Species: Human
    Sample Types: Serum
  33. Significant differences in B-cell subpopulations characterize patients with chronic graft-versus-host disease-associated dysgammaglobulinemia.
    Authors: Kuzmina Z, Greinix HT, Weigl R, Kormoczi U, Rottal A, Frantal S, Eder S, Pickl WF
    Blood, 2011;117(7):2265-74.
    Species: Human
    Sample Types: Plasma
  34. B-cell reconstitution and BAFF after alemtuzumab (Campath-1H) treatment of multiple sclerosis.
    Authors: Thompson SA, Jones JL, Cox AL, Compston DA, Coles AJ
    J. Clin. Immunol., 2010;30(1):99-105.
    Species: Human
    Sample Types: Serum
  35. Peripheral blood B cell subset skewing is associated with altered cell cycling and intrinsic resistance to apoptosis and reflects a state of immune activation in chronic hepatitis C virus infection.
    Authors: Sugalski JM, Rodriguez B, Moir S
    J. Immunol., 2010;185(5):3019-27.
    Species: Human
    Sample Types: Serum
  36. Rituximab mediates a strong elevation of B-cell-activating factor associated with increased pathogen-specific IgG but not autoantibodies in pemphigus vulgaris.
    Authors: Nagel A, Podstawa E, Eickmann M, Muller HH, Hertl M, Eming R
    J. Invest. Dermatol., 2009;129(9):2202-10.
    Species: Human
    Sample Types: Serum
  37. BAFF is increased in renal transplant patients following treatment with alemtuzumab.
    Authors: Bloom D, Chang Z, Pauly K
    Am. J. Transplant., 2009;9(8):1835-45.
    Species: Human
    Sample Types: Serum
  38. Elevated B-cell activating factor BAFF, but not APRIL, correlates with CSF cerebellar autoantibodies in pediatric opsoclonus-myoclonus syndrome.
    Authors: Fuhlhuber V, Bick S, Kirsten A, Hahn A, Gerriets T, Tschernatsch M, Kaps M, Preissner KT, Blaes F, Altenkamper S
    J. Neuroimmunol., 2009;210(1):87-91.
    Species: Human
    Sample Types: Serum
  39. Functional interaction of plasmacytoid dendritic cells with multiple myeloma cells: a therapeutic target.
    Authors: Chauhan D, Singh AV, Brahmandam M, Carrasco R, Bandi M, Hideshima T, Bianchi G, Podar K, Tai YT, Mitsiades C, Raje N, Jaye DL, Kumar SK, Richardson P, Munshi N, Anderson KC
    Cancer Cell, 2009;16(4):309-23.
    Species: Human
    Sample Types: Cell Culture Supernates
  40. Elevation of B cell-activating factor belonging to the tumour necrosis factor [corrected] family (BAFF) in haemophilia A patients with inhibitor.
    Authors: Takeda T, Sakurai Y, Tatsumi K, Kato J, Kasuda S, Yoshioka A, Shima M
    Thromb. Haemost., 2009;101(2):408-10.
    Species: Human
    Sample Types: Plasma
  41. BAFF may modulate the rate of B-cell repopulation after rituximab therapy for acute renal transplant rejection.
    Authors: Zarkhin V, Li L, Sarwal MM
    Transplantation, 2009;88(10):1229-30.
    Species: Human
    Sample Types: Serum
  42. BAFF and APRIL expression in B-cell chronic lymphocytic leukemia: correlation with biological and clinical features.
    Authors: Bojarska-Junak A, Hus I, Chocholska S, Wasik-Szczepanek E, Sieklucka M, Dmoszynska A, Rolinski J
    Leuk. Res., 2009;33(10):1319-27.
    Species: Human
    Sample Types: Plasma
  43. Altered B-cell homeostasis and excess BAFF in human chronic graft-versus-host disease.
    Authors: Sarantopoulos S, Stevenson KE, Kim HT, Cutler CS, Bhuiya NS, Schowalter M, Ho VT, Alyea EP, Koreth J, Blazar BR, Soiffer RJ, Antin JH, Ritz J
    Blood, 2009;113(16):3865-74.
    Species: Human
    Sample Types: Plasma
  44. Evidence of a role for B cell-activating factor of the TNF family in the pathogenesis of chronic rhinosinusitis with nasal polyps.
    Authors: Kato A, Peters A, Suh L, Carter R, Harris KE, Chandra R, Conley D, Grammer LC, Kern R, Schleimer RP
    J. Allergy Clin. Immunol., 2008;121(6):1385-92, 1392.
    Species: Human
    Sample Types: Nasal Lavage
  45. Abnormal cytokine production by bone marrow stromal cells of multiple myeloma patients in response to RPMI8226 myeloma cells.
    Authors: Zdzisinska B, Bojarska-Junak A, Dmoszynska A, Kandefer-Szerszen M
    Arch. Immunol. Ther. Exp. (Warsz.), 2008;56(3):207-21.
    Species: Human
    Sample Types: Cell Culture Supernates
  46. Targeting MEK1/2 blocks osteoclast differentiation, function and cytokine secretion in multiple myeloma.
    Authors: Breitkreutz I, Raab MS, Vallet S, Hideshima T, Raje N, Chauhan D, Munshi NC, Richardson PG, Anderson KC
    Br. J. Haematol., 2007;139(1):55-63.
    Species: Human
    Sample Types: Cell Culture Supernates
  47. Role of B-cell-activating factor in adhesion and growth of human multiple myeloma cells in the bone marrow microenvironment.
    Authors: Tai YT, Li XF, Breitkreutz I, Song W, Neri P, Catley L, Podar K, Hideshima T, Chauhan D, Raje N, Schlossman R, Richardson P, Munshi NC, Anderson KC
    Cancer Res., 2006;66(13):6675-82.
    Species: Human
    Sample Types: Cell Culture Supernates

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Human BAFF/BLyS/TNFSF13B Quantikine ELISA Kit
By Anonymous on 12/04/2017
Sample Tested: Serum