BAFF (B-cell activating factor), also known as BLyS, TALL-1, THANK, and TNFSF13B, is a TNF superfamily ligand that plays a critical role in the development and survival of B lineage cells. It can be expressed as a homotrimer or as a heteromer in association with the related protein APRIL. It is produced by many hematopoietic cell types, and a soluble form can be released by activated neutrophils. Both BAFF and APRIL signal through the receptors BCMA and TACI, and BAFF additionally signals through BAFF R. Mice that overexpress BAFF exhibit elevated B cell numbers, increased formation and size of germinal centers, and symptoms of autoimmunity. In addition, BAFF costimulates T cell activation, promotes a Th1 biased immune response, and promotes the expansion of Treg cells.
Human BAFF/BLyS/TNFSF13B Quantikine ELISA Kit
R&D Systems | Catalog # DBLYS0
Key Product Details
Assay Length
Sample Type & Volume Required Per Well
Sensitivity
11.9 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Assay Range
62.5-4000 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Human BAFF/BLyS/TNFSF13B Quantikine ELISA Kit
Product Specifications
Measurement
Detection Method
Species
Specificity
Cross-reactivity
Interference
Sample Values
| Sample Type | Mean (pg/mL) | Range (pg/mL) | Standard Deviation (pg/mL) |
| Serum (n=36) | 1169 | 671-2447 | 283 |
| EDTA plasma | 1000 | 609-1946 | 236 |
| Heparin plasma (n=36) | 1002 | 576-2132 | 253 |
Cell Culture Supernates - Human peripheral blood cells were cultured in DMEM supplemented with 5% fetal calf serum, 50 mM b-mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 mg/mL streptomycin sulfate. Cells were cultured unstimulated or stimulated with 10 mg/mL PHA. Aliquots of the cell culture supernates were removed and assayed for levels of natural human BAFF.
| Condition | Day 1 (pg/mL) | Day 2 (pg/mL) |
| Unstimulated | ND | ND |
| Stimulated | ND | 45.9 |
| Cell Line | Value (pg/mL) |
| THP-1 human acute monocytic leukemia | 100 |
| U937 human histiocytic lymphoma (7 day culture) | 1251 |
| U937 human histiocytic lymphoma (10 day culture) | 18,850 |
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 141 | 462 | 871 | 156 | 482 | 955 |
| Standard Deviation | 7.53 | 18.8 | 37.5 | 13.5 | 41.7 | 96.7 |
| CV% | 5.3 | 4.1 | 4.3 | 8.7 | 8.7 | 10.1 |
EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 458 | 1371 | 2545 | 498 | 1476 | 2854 |
| Standard Deviation | 17.5 | 82.8 | 123 | 38.9 | 106 | 256 |
| CV% | 3.8 | 6.0 | 4.8 | 7.8 | 7.2 | 9.0 |
Recovery for Human BAFF/BLyS/TNFSF13B Quantikine ELISA Kit
The recovery of BAFF spiked to levels throughout the range of the assay in various matrices was evaluated. One sample showed < 50% recovery and was not included in the data.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 101 | 87-112 |
| EDTA Plasma (n=4) | 101 | 85-114 |
| Heparin Plasma (n=4) | 98 | 86-110 |
| Serum (n=4) | 101 | 86-114 |
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of BAFF were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human BAFF/BLyS/TNFSF13B Quantikine ELISA Kit
Human BAFF/BLyS/TNFSF13B ELISA Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: BAFF/BLyS/TNFSF13B
Long Name
Alternate Names
Gene Symbol
Additional BAFF/BLyS/TNFSF13B Products
Product Documents for Human BAFF/BLyS/TNFSF13B Quantikine ELISA Kit
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human BAFF/BLyS/TNFSF13B Quantikine ELISA Kit
For research use only
Citations for Human BAFF/BLyS/TNFSF13B Quantikine ELISA Kit
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Sample Tested: SerumVerified Customer | Posted 12/04/2017
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