Human BASP1 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human BASP1 in Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human BASP1
    Gly2-Ala45
    Accession # P80723
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human BASP1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and DU145 human prostate carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Human BASP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6479) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for BASP1 at approximately 60 kDa, possibly a higher molecular weight isoform or a product of SUMOyation. Lower molecular weight isoforms were also observed. This experiment was conducted under reducing conditions on a 4-20% SDS-PAGE gel and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BASP1

BASP1 (Brain acid soluble protein 1; also NAP-22) is a transcriptional cosuppressor that belongs to the BASP1 family of proteins. Although its predicted MW is 23 kDa, BASP1 will show multiple bands in SDS-Page. First, BASP1 forms oligomers in SDS, ranging in size from 30-150 kDa. Second, varying concentrations of SDS will change the apparent BASP1 MW from 56 kDa in 8% SDS-Page to 41 kDa in 13% SDS-Page. At least two forms of BASP1 apparently exist in human, one that is 48-52 kDa, and another that is 32-40 kDa in size. They are not functionally equivalent. BASP1 can be SUMOylated that add 20 kDa in apparent MW. There are also N-terminal fragments termed BIRPs that run from 30-50 kDa in 12% acidic SDS-Page. BASP1 is expressed in neurons, renal podocytes and spermatids. It binds to the cytoplasmic side of the plasmalemma via its myristroylate adduct, and undergoes nuclear translocation when SUMOylated. Human BASP1 is 227 amino acids (aa) in length. It contains a myristoylation site at Gly2, SUMOylation sites at Lys79 and 84, at least eight utilized phosphorylation sites, and an NLS. One splice variant shows a deletion of aa 88-141. Over aa 1-45, human BASP1 shares 96% and 91% aa identity with mouse and rat BASP1, respectively.

  • Long Name:
    Brain Acid Soluble Protein 1
  • Entrez Gene IDs:
    10409 (Human); 70350 (Mouse); 64160 (Rat)
  • Alternate Names:
    BASP1; brain abundant, membrane attached signal protein 1,22 kDa neuronal tissue-enriched acidic protein; brain acid-soluble protein 1; CAP23; CAP-23; CAP23brain acid soluble protein 1; NAP22; NAP-22; NAP22MGC8555; Neuronal axonal membrane protein NAP-22; neuronal tissue-enriched acidic protein
Related Research Areas
Isotype Controls
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Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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