Human Bcl9-2 Antibody

Detection of Human Bcl9‑2 by Western Blot. Western blot shows lysates of A172 human glioblastoma cell line and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Human Bcl9-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4967) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Bcl9-2 at approximately 190 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Bcl9-2 by Western Blot BCL9L expression in primary pancreatic tumor tissue and cell lines(A) Box-and-whisker plot showing results from BCL9L mRNA expression analysis by qRT-PCR in tissue samples derived from primary human pancreatic tumors (n = 26 cases), chronic pancreatitis (n = 6 cases) and normal pancreas (n = 13 cases). Expression was normalized to ribosomal protein, large, P0 (RPLP0) mRNA levels. Bars represent median and 2nd and 3rd quartiles (boxes) as well as minimum and maximum values (whiskers). **p ≤ 0.01, ***p ≤ 0.001 (Student's t-test). (B) BCL9L mRNA levels in pancreatic cancer (8988t, IMIM PC1, IMIM PC2, MiaPaca-2, Panc1, S2-007, S2-028) and control (HEK 293) cell lines. (C) Staining of tissue microarrays (TMA) for BCL9L expression using immunohistochemistry. Enhanced expression was seen in PDAC tissues (bottom panels) compared to normal pancreas (upper panels). Staining intensity in PDAC tissues increased with tumor grade (bottom left: G1, bottom middle: G2, bottom right: G3). (D) BCL9L staining intensity of tissue microarrays was quantitatively evaluated and scored in normal and PDAC cases as described in Materials and Methods. Scores significantly increased with the progression to less differentiated PDAC (grade 2 and 3). ***p ≤ 0.001 (Mann-Whitney and Kruskal-Wallis non parametric test) (E) BCL9L protein expression in pancreatic cancer and control (HPNE) cell lines was quantified by western blotting. Detection of alpha -tubulin was used as a loading control. Shown is a representative image of 3 experiments. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/27713160), licensed under a CC-BY license. Not internally tested by R&D Systems.