Human C2CD3 Antibody Summary
Accession # Q4AC94
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
C2CD3 in HeLa Human Cell Line. C2CD3 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human C2CD3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7348) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
C2CD3 (C2 domain-containing protein 3) is a 260 kDa (predicted) intracellular protein that apparently belongs to no known structural protein family. It is ubiquitously expressed, and plays a role in cilia formation and associated hedgehog signaling. In particular, C2CD3 is localized to the basal body, and appears to promote Gli3 proteolytic processing into a truncated transcriptional repressor. Human C2CD3 is 2353 amino acids (aa) in length. It contains two C2 domains (aa 1163-1289 and 1622-1728) that participate in Ca++-dependent protein-lipid and protein-protein interactions. Three utilized phosphorylation sites exist at Thr1130, Ser2114 and Ser 2132. There are multiple potential splice variants. One possesses a three aa substitution for aa 1961-2353, a second shows a deletion of aa 1364-1382 coupled to a 27 aa substitution for aa 1881-2353, a third contains a seven aa substitution for aa 1961-2353, and a fourth contains a 34 aa substitution for aa 1888-2353. Over aa 1-109, human C2CD3 shares 94% aa identity with mouse C2CD3.
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