Human C2CD3 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human C2CD3 in direct ELISAs.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human C2CD3
    Met1-Asp109
    Accession # Q4AC94
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
C2CD3 in HeLa Human Cell Line. C2CD3 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human C2CD3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7348) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: C2CD3

C2CD3 (C2 domain-containing protein 3) is a 260 kDa (predicted) intracellular protein that apparently belongs to no known structural protein family. It is ubiquitously expressed, and plays a role in cilia formation and associated hedgehog signaling. In particular, C2CD3 is localized to the basal body, and appears to promote Gli3 proteolytic processing into a truncated transcriptional repressor. Human C2CD3 is 2353 amino acids (aa) in length. It contains two C2 domains (aa 1163-1289 and 1622-1728) that participate in Ca++-dependent protein-lipid and protein-protein interactions. Three utilized phosphorylation sites exist at Thr1130, Ser2114 and Ser 2132. There are multiple potential splice variants. One possesses a three aa substitution for aa 1961-2353, a second shows a deletion of aa 1364-1382 coupled to a 27 aa substitution for aa 1881-2353, a third contains a seven aa substitution for aa 1961-2353, and a fourth contains a 34 aa substitution for aa 1888-2353. Over aa 1-109, human C2CD3 shares 94% aa identity with mouse C2CD3.

  • Long Name:
    C2 Domain-containing Protein 3
  • Entrez Gene IDs:
    26005 (Human); 277939 (Mouse); 293148 (Rat)
  • Alternate Names:
    C2 calcium-dependent domain containing 3; C2CD3
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