Insulin and the IGFs are members of the insulin family of molecules. IGF-I and II are structurally homologous to proinsulin, and can be thought of as taking the shape of an exaggerated, inverted letter G. Intrachain disulfide bonds link sections of the G. Insulin processes out part of the G, leaving two parallel, but disulfide linked segments; the IGFs only process at the ends, retaining the basic configuration.
Best Seller
Human/Canine/Porcine Insulin Quantikine ELISA Kit
R&D Systems | Catalog # DINS00
Select the "Bulk Orders" button to request additional sizes or formulations.
Loading...
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL)
Sensitivity
2.15 pmol/L
Assay Range
15.6-500 pmol/L (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Loading...
Product Summary for Human/Canine/Porcine Insulin Quantikine ELISA Kit
The Quantikine Human/Canine/Porcine Insulin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure Insulin in cell culture supernates, serum, and plasma. It contains S. cerevisiae-expressed recombinant human Insulin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural Insulin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring Insulin.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Species
Human, Porcine, Canine
Specificity
Recombinant human Insulin and natural human, canine, and porcine Insulin. This assay cross-reacts 0.25% with Human Proinsulin.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity observed with 1 or more species tested.
Interference
No significant interference observed with available related molecules.
Sample Values
Serum/Plasma - Samples from apparently healthy, fasting volunteers were evaluated for the
presence of human/canine/porcine Insulin in this assay. No medical histories were available for the
donors used in this study.
ND=Non-detectable
ND=Non-detectable
| Human Samples | Mean (pmol/L) | Range (pmol/L) | Standard Deviation (pmol/L) |
| Serum (n=34) | 58.0 | 17.2-218 | 41 |
| EDTA plasma (n=34) | 60.3 | 15.8-208 | 39 |
| Heparin plasma (n=34) | 59.4 | 16.4-190 | 45 |
| Canine Samples | Mean (pmol/L) | Range (pmol/L) | Standard Deviation (pmol/L) |
| Serum (n=10) | 58.8 | 16.7-190 | 52 |
| Heparin plasma (n=5) | 78.3 | 21.5-146 | 56 |
| Canine Samples | Mean of Detectable (pmol/L) | % Detectable | Range (pmol/L) |
| EDTA plasma (n=5) | 96.4 | 80 | ND-145 |
| Porcine Samples | Mean of Detectable (pmol/L) | % Detectable | Range (pmol/L) |
| Serum (n=10) | 22.6 | 90 | ND-34.3 |
| EDTA plasma (n=5) | 27.1 | 80 | ND-40.8 |
| Heparin plasma (n=5) | 30.4 | 80 | ND-40.9 |
Cell Culture Supernates - Human peripheral blood mononuclear cells (PBL) were cultured in DMEM
supplemented with 5% fetal bovine serum, 50 μM beta -mercaptoethanol, 2 mM L-glutamine, 100 U/mL
penicillin, and 100 μg/mL streptomycin sulfate. Cells were cultured unstimulated or stimulated with
10 μg/mL PHA for 1 and 5 days. Aliquots of the cell culture supernates were removed and assayed
for human/canine/porcine Insulin. No detectable levels were observed.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pmol/L) | 66.8 | 158 | 299 | 62.2 | 148 | 288 |
| Standard Deviation | 2.58 | 5.89 | 11.9 | 4.60 | 10.2 | 21.5 |
| CV% | 3.9 | 3.7 | 4.0 | 7.4 | 6.9 | 7.5 |
Recovery for Human/Canine/Porcine Insulin Quantikine ELISA Kit
The recovery of Insulin spiked to levels throughout the range of the assay in various matrices was evaluated
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 95 | 85-108 |
| EDTA Plasma (n=4) | 95 | 86-102 |
| Heparin Plasma (n=4) | 92 | 85-105 |
| Serum (n=4) | 96 | 87-103 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Insulin were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human/Canine/Porcine Insulin Quantikine ELISA Kit
Human/Porcine/Canine Insulin ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Insulin
Alternate Names
IDDM2, ILPR, INS, IRDN, MODY10
Gene Symbol
INS
Additional Insulin Products
Product Documents for Human/Canine/Porcine Insulin Quantikine ELISA Kit
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human/Canine/Porcine Insulin Quantikine ELISA Kit
For research use only
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.Citations for Human/Canine/Porcine Insulin Quantikine ELISA Kit
Powered by Bioz
Customer Reviews for Human/Canine/Porcine Insulin Quantikine ELISA Kit (3)
4.7 out of 5
3 Customer Ratings
Have you used Human/Canine/Porcine Insulin Quantikine ELISA Kit?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Customer Images
Showing
1
-
3 of
3 reviews
Showing All
Filter By:
-
Sample Tested: human floxp embryonal stem cell culture mediumVerified Customer | Posted 11/22/2021
-
Sample Tested: Cell Culture MediaVerified Customer | Posted 10/25/2018
-
Sample Tested: Heparin PlasmaVerified Customer | Posted 04/12/2018Ran here according to manufacturers instructions vs a Duoset Kit (#DY8056-05) before purchasing multiple kits for large study. Human heparinised plasma from an IVGTT, ran in both plates at the same time, by the same researcher on the same aliquots. Broadly - Duoset signal was stronger throughout, and resultant IVGTT curve was smoother. Quantikine kit may be underexposed with 30 minutes substrate development in the Quantikine instructions? We've moved forward with the Duosets.
There are no reviews that match your criteria.
Protocols
View specific protocols for Human/Canine/Porcine Insulin Quantikine ELISA Kit (DINS00):
Refer to the product for complete assay procedure.
Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Aspirate each well and wash, repeating once more for a total of 2 washes.
- Add 150 µL of Assay Diluent to each well.
- Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
- Aspirate and wash 4 times.
- Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
- Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
150 µL Assay Diluent
50 µL Standard, Control, or Sample
200 µL Conjugate
200 µL Substrate Solution
50 µL Stop Solution
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
