Human Carbonic Anhydrase XIII/CA13 Antibody

Catalog # Availability Size / Price Qty
AF2194
AF2194-SP
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Human Carbonic Anhydrase XIII/CA13 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Carbonic Anhydrase XIII/CA13 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 15% cross‑reactivity with recombinant human (rh) CA1, rhCA2, and rhCA3 is observed and 5% cross‑reactivity with rhCA4, rhCA8, rhCA9, rhCA10, rhCA12, and rhCA14 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Carbonic Anhydrase XIII/CA13
Ser2-His262
Accession # Q8N1Q1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Carbonic Anhydrase XIII (Catalog # 2194-CA)
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Carbonic Anhydrase XIII (Catalog # 2194-CA), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Carbonic Anhydrase XIII/CA13

Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6th International Conference on the CAs, June 20‑25, 2003, Slovakia) ranged from the use of CAs as markers for tumor and hypoxia in the clinic, as a nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. CA13 is a cytosolic enzyme with a unique and widespread distribution pattern as compared to the other cytosolic CAs, indicating that it may play important physiological roles in several organs (2).

References
  1. Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
  2. Lehtonen, J. et al. (2003) J. Biol. Chem. 279: 2719.
Entrez Gene IDs
377677 (Human)
Alternate Names
CA13; carbonic anhydrase 13; Carbonic Anhydrase XIII; carbonic anhydrase XIIICarbonate dehydratase XIII; CAXIII; CA-XIII; EC 4.2.1.1; FLJ37995; MGC59868

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