Human Cathepsin H Antibody

Catalog # Availability Size / Price Qty
Detection of Human Cathepsin H by Western Blot.
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Product Details
Citations (1)
Supplemental Products

Human Cathepsin H Antibody Summary

Species Reactivity
Detects human Cathepsin H in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant mouse Cathepsin H is observed, and less than 1% cross-reactivity with recombinant human Cathepsin L2 is observed.
Polyclonal Sheep IgG
Antigen Affinity-purified
Chinese hamster ovary cell line CHO-derived recombinant human Cathepsin H
Accession # CAA34734
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Cathepsin H antibody by Western Blot. View Larger

Detection of Human Cathepsin H by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, A431 human epithelial carcinoma cell line, human kidney tissue, and human lung tissue. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human Cathepsin H Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7516) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for Cathepsin H at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Knockout Validated View Larger

Western Blot Shows Human Cathepsin H Specificity Using Knockout Cell Line. Western blot shows lysates of HEK293T human embryonic kidney cell line and Cathepsin H knockout HEK293T cell line (KO). Nitrocellulose membrane was probed with 0.2 µg/mL of Sheep Anti-Human Cathepsin H Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7516) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody. A specific band was detected for Cathepsin H at approximately 25 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See for additional details.

Reconstitution Calculator

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Preparation and Storage

Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cathepsin H

Cathepsin H (CTSH; alos CPSB) is a 28-30 kDa ubiquitously expressed member of the peptidase C1 family of cysteine lysosomal enzymes. Depending upon the isoform, it may either be secreted or retained in the lysosomes. Although this family of enzymes plays a key role in general intracellular molecule degradation, CTSH is also known to participate in other activities. Such activities include the activation of progranzyme B, the late stage processing of pulmonary surfactant protein B, and the cleavage, in endosomes, of TLR3, allowing for subsequent TLR signaling. Human preproCTSH is 335 amino acids (aa) in length. It contains one 22 aa signal peptide, a 75 aa prosequence (aa 23-97), an intervening "mini fragment" (aa 98-105), a second prosequence (aa 106-115), and a 220 aa C-terminal mature region that could be further cleaved after Asn292 into two peptide fragments. CTSH is self-activating. The 40-42 kDa proprecursor (aa 23-335) is cleaved thrice to initially generate a 30 kDa intermediate form, followed by a 28 kDa mature form that is covalently attached to the aforementioned "mini fragment". Further processing at the C-terminus may create a smaller 24-25 kDa mature segment. CTSH is unusual in that it possess both aminopeptidase and endopeptidase activity. While endopeptidase activity is intrinsic to the mature region, aminopeptidase activity requires the presence of a covalently-linked "mini fragment". There is one splice variant that shows a deletion of aa 11-22. Although this occurs within the signal sequence, the molecule is secreted and shows high enzymatic activity. Over aa 23-335, human CTSH shares 84% aa sequence identity with mouse CTSH.

Entrez Gene IDs
1512 (Human); 13036 (Mouse)
Alternate Names
ACC-4; ACC-5; aleurain; cathepsin B3; cathepsin BA; Cathepsin H; CPSB; CTSH; DKFZp686B24257; EC 3.4.22; EC; MGC1519; minichain; N-benzoylarginine-beta-naphthylamide hydrolase

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Citation for Human Cathepsin H Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Distinct functions of macrophage-derived and cancer cell-derived cathepsin Z combine to promote tumor malignancy via interactions with the extracellular matrix.
    Authors: Akkari L, Gocheva V, Kester J, Hunter K, Quick M, Sevenich L, Wang H, Peters C, Tang L, Klimstra D, Reinheckel T, Joyce J
    Genes Dev, 2014;28(19):2134-50.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P


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