Human CCL18/PARC Biotinylated Antibody Summary
Accession # P55774
Human CCL18/PARC Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of CCL18/PARC in Human Macrophages by Flow Cytometry. Macrophages were generated from positively selected (MAGH105) CD14+ human PBMC, cultured with 50 ng/ml rhM-CSF (216-MC), 10 ng/ml rhIL-10 (217-IL) and 10 ng/ml rhIL-4 (204-IL) for 6 days, then treated with 3nM Monensin overnight and stained with (A) Goat Anti-Human CCL18/PARC Biotinylated Polyclonal Antibody (Catalog # BAF394) or (B) control antibody (BAF108), followed by Allophycocyanin-conjugated Streptavidin (F0050) and Mouse anti-Human CD163 Alexa Fluor® 488-conjugated Monoclonal Antibody (FAB1607G). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our Staining Intracellular Molecules protocol.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
CCL18, also known as alternative macrophage activation-associated CC chemokine (AMAC)-1, macrophage inflammatory protein (MIP)-4, and dendritic cell chemokine (DC-CK1), is a novel CC chemokine that is highly homologous to MIP-1 alpha (61% amino acid (aa) sequence identity). CCL18 cDNA encodes an 89 aa residue precursor protein with a 20 aa putative signal peptide that is cleaved to generate a 69 aa residue mature protein which lacks potential glycosylation sites. In vitro, CCL18 mRNA expression is induced in alternatively activated macrophages by Th2 cytokines such as IL-4, IL-10, and IL-13, and inhibited by IFN‑ gamma. CCL18 mRNA is also expressed by GM-CSF/IL-4-induced monocyte-derived dendritic cells. In vivo, CCL18 is highly expressed in lung and placenta but is not expressed in epidermal Langerhans cells. Recombinant CCL18 has been shown to chemoattract naive T cells but not monocytes or neutrophils.
Citations for Human CCL18/PARC Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 4
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Prominent Levels of the Profibrotic Chemokine CCL18 during Peritonitis: In Vitro Downregulation by Vitamin D Receptor Agonists
Authors: M Ossorio, V Martínez, MA Bajo, G Del Peso, MJ Castro, S Romero, R Selgas, T Bellón
Biomed Res Int, 2018;2018(0):6415892.
Sample Types: Whole Cells
Multiplex serum protein analysis reveals potential mechanisms and markers of response to hyperimmune caprine serum in systemic sclerosis
Authors: N Quillinan, KE Clark, B Youl, J Vernes, D McIntosh, S Haq, CP Denton
Arthritis Res. Ther, 2017;19(1):45.
Sample Types: Serum
Applications: ELISA Development (Detection)
Potential biomarkers of osteonecrosis in Gaucher disease.
Authors: Pavlova EV, Deegan PB, Tindall J, McFarlane I, Mehta A, Hughes D, Wraith JE, Cox TM
Blood Cells Mol. Dis., 2011;46(1):27-33.
Sample Types: Serum
Applications: Luminex Development
Osseous manifestations of adult Gaucher disease in the era of enzyme replacement therapy.
Authors: Deegan PB, Pavlova E, Tindall J
Medicine (Baltimore), 2011;90(1):52-60.
Sample Types: Plasma
Applications: Complex Application
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