Human CCL22/MDC Biotinylated Antibody

R&D Systems | Catalog # BAF336

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Guinea Pig

Applications

Validated:

Western Blot, ELISA Detection (Matched Antibody Pair)

Cited:

ELISA Detection, ELISA Development

Label

Biotin

Antibody Source

Polyclonal Chicken IgY
View all CCL22/MDC Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CCL22/MDC (R&D Systems, Catalog # 336-MD)
Gly25-Gln93
Accession # O00626

Specificity

Detects human CCL22/MDC in ELISAs and Western blots. In sandwich immunoassays, less than 0.03% cross-reactivity with recombinant mouse TARC, recombinant human (rh) TARC, and rh6Ckine is observed.

Clonality

Polyclonal

Host

Chicken

Isotype

IgY

Applications for Human CCL22/MDC Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human CCL22/MDC (Catalog # 336-MD)

Human CCL22/MDC Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Human CCL22/MDC Antibody (Catalog # MAB336)
  • Standard: Recombinant Human CCL22/MDC Protein (Catalog # 336-MD)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 4 using BAF336 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified from egg yolks

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL22/MDC

CCL22, also named stimulated T cell chemotactic protein (STCP-1), is a CC chemokine initially isolated from clones of monocyte-derived macrophages. Human CCL22 cDNA encodes a precursor protein of 93 amino acid residues with a 24 amino acid residue predicted signal peptide that is cleaved to yield a 69 amino acid residue mature 8 kDa protein. At the amino acid sequence level, CCL22 shows less than 35% identity to other CC chemokine family members. Human CCL22 is expressed in dendritic cells, macrophages and activated monocytes. In addition, CCL22 expression is also detected in the tissues of thymus, lymph node and appendix. The gene for human CCL22 has been mapped to chromosome 16 rather than chromosome 17 where the genes for many human CC chemokines are clustered. Recombinant or chemically synthesized mature CCL22 has been shown to induce chemotaxis or Ca2+ mobilization in dendritic cells, IL-2 activated NK cells, and activated T lymphocytes. A CD8+ T lymphocyte-derived secreted soluble activity that suppresses infection by primary non-syncytium-inducing and syncytium-inducing HIV-1 isolates and the T-cell line-adapted isolate HIV-1IIIB, has been identified as CCL22. Based on amino-terminal sequence analysis, the major CD8+ T lymphocyte-derived CCL22 protein yielded an amino-terminal sequence of YGANM, which is two amino acid residues shorter than the predicted mature CCL22. The difference in potency between the two mature CCL22 isoforms has not been determined.

References

  1. Godiska, R. et al. (1997) J. Exp. Med. 185:1595.
  2. Chang, M-S. et al. (1997) J. Biol. Chem. 272:25229.
  3. Pal, R. et al. (1997) Science 278:5338.

Alternate Names

ABCD-1, MDC, STCP-1

Entrez Gene IDs

6367 (Human); 20299 (Mouse)

Gene Symbol

CCL22

UniProt

O00626

Additional CCL22/MDC Products

Product Documents for Human CCL22/MDC Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CCL22/MDC Biotinylated Antibody

For research use only

Citations for Human CCL22/MDC Biotinylated Antibody

Customer Reviews for Human CCL22/MDC Biotinylated Antibody (2)

4 out of 5
2 Customer Ratings
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  • Name: Anonymous
    Application: ELISA
    Sample Tested: See PMID 21801246
    Species: Human
    Verified Customer | Posted 01/13/2015
  • Name: Anonymous
    Application: ELISA
    Sample Tested: See PMID 23517039
    Species: Human
    Verified Customer | Posted 01/13/2015

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