CCL22, also named stimulated T cell chemotactic protein (STCP-1), is a CC chemokine initially isolated from clones of monocyte-derived macrophages. Human CCL22 cDNA encodes a precursor protein of 93 amino acid residues with a 24 amino acid residue predicted signal peptide that is cleaved to yield a 69 amino acid residue mature 8 kDa protein. At the amino acid sequence level, CCL22 shows less than 35% identity to other CC chemokine family members. Human CCL22 is expressed in dendritic cells, macrophages and activated monocytes. In addition, CCL22 expression is also detected in the tissues of thymus, lymph node and appendix. The gene for human CCL22 has been mapped to chromosome 16 rather than chromosome 17 where the genes for many human CC chemokines are clustered. Recombinant or chemically synthesized mature CCL22 has been shown to induce chemotaxis or Ca2+ mobilization in dendritic cells, IL-2 activated NK cells, and activated T lymphocytes. A CD8+ T lymphocyte-derived secreted soluble activity that suppresses infection by primary non-syncytium-inducing and syncytium-inducing HIV-1 isolates and the T-cell line-adapted isolate HIV-1IIIB, has been identified as CCL22. Based on amino-terminal sequence analysis, the major CD8+ T lymphocyte-derived CCL22 protein yielded an amino-terminal sequence of YGANM, which is two amino acid residues shorter than the predicted mature CCL22. The difference in potency between the two mature CCL22 isoforms has not been determined.
Human CCL22/MDC Antibody [Biotin]
R&D Systems | Catalog # BAF336
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Guinea Pig
Applications
Validated:
Western Blot, ELISA Detection (Matched Antibody Pair)
Cited:
ELISA Detection, ELISA Development
Label
Biotin
Antibody Source
Polyclonal Chicken IgY
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Product Specifications
Immunogen
E. coli-derived recombinant human CCL22/MDC (R&D Systems, Catalog # 336-MD)
Gly25-Gln93
Accession # O00626
Gly25-Gln93
Accession # O00626
Specificity
Detects human CCL22/MDC in ELISAs and Western blots. In sandwich immunoassays, less than 0.03% cross-reactivity with recombinant mouse TARC, recombinant human (rh) TARC, and rh6Ckine is observed.
Clonality
Polyclonal
Host
Chicken
Isotype
IgY
Scientific Data Images for Human CCL22/MDC Antibody [Biotin]
Human CCL22 / MDC ELISA Standard Curve
Recombinant Human CCL22/MDC (Catalog # 336-MD) was serially diluted and captured by Mouse Anti-Human CCL22/MDC Monoclonal Antibody (Catalog # MAB336) coated on a Clear Polystyrene Microplate (Catalog # DY990). Chicken Anti-Human CCL22/MDC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF336) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)Applications for Human CCL22/MDC Antibody [Biotin]
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human CCL22/MDC (Catalog # 336-MD)
Sample: Recombinant Human CCL22/MDC (Catalog # 336-MD)
Human CCL22/MDC Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 4 using BAF336 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified from egg yolks
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: CCL22/MDC
References
- Godiska, R. et al. (1997) J. Exp. Med. 185:1595.
- Chang, M-S. et al. (1997) J. Biol. Chem. 272:25229.
- Pal, R. et al. (1997) Science 278:5338.
Alternate Names
ABCD-1, MDC, STCP-1
Gene Symbol
CCL22
UniProt
Additional CCL22/MDC Products
Product Documents for Human CCL22/MDC Antibody [Biotin]
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human CCL22/MDC Antibody [Biotin]
For research use only
Citations for Human CCL22/MDC Antibody [Biotin]
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Customer Reviews for Human CCL22/MDC Antibody [Biotin] (2)
4 out of 5
2 Customer Ratings
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Application: ELISASample Tested: See PMID 21801246Species: HumanVerified Customer | Posted 01/13/2015
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
