Human CCL22/MDC Antibody

R&D Systems | Catalog # AF336

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Neutralization

Cited:

Neutralization, Flow Cytometry, Bioassay

Label

Unconjugated

Antibody Source

Polyclonal Chicken IgY
View all CCL22/MDC Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CCL22/MDC (R&D Systems, Catalog # 336-MD)
Gly25-Gln93
Accession # O00626

Specificity

Detects human CCL22/MDC in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse MDC and recombinant human MPIF-1 is observed.

Clonality

Polyclonal

Host

Chicken

Isotype

IgY

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human CCL22/MDC Antibody

Chemotaxis Induced by CCL22/MDC and Neutralization by Human CCL22/MDC Antibody.

Chemotaxis Induced by CCL22/MDC and Neutralization by Human CCL22/MDC Antibody.

Recombinant Human CCL22/MDC (Catalog # 336-MD) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL22/MDC (0.03 µg/mL) is neutralized (green line) by increasing concentrations of Chicken Anti-Human CCL22/MDC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF336). The ND50 is typically 0.4-2.0 µg/mL.

Applications for Human CCL22/MDC Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human CCL22/MDC (Catalog # 336-MD)

Neutralization

Measured by its ability to neutralize CCL22/MDC-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR4. The Neutralization Dose (ND50) is typically 0.4-2.0 µg/mL in the presence of 0.03 µg/mL Recombinant Human CCL22/MDC.

Reviewed Applications

Read 1 review rated 3 using AF336 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified from egg yolks

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL22/MDC

CCL22, also named stimulated T cell chemotactic protein (STCP-1), is a CC chemokine initially isolated from clones of monocyte-derived macrophages. Human CCL22 cDNA encodes a precursor protein of 93 amino acid residues with a 24 amino acid residue predicted signal peptide that is cleaved to yield a 69 amino acid residue mature 8 kDa protein. At the amino acid sequence level, CCL22 shows less than 35% identity to other CC chemokine family members. Human CCL22 is expressed in dendritic cells, macrophages and activated monocytes. In addition, CCL22 expression is also detected in the tissues of thymus, lymph node and appendix. The gene for human CCL22 has been mapped to chromosome 16 rather than chromosome 17 where the genes for many human CC chemokines are clustered. Recombinant or chemically synthesized mature CCL22 has been shown to induce chemotaxis or Ca2+ mobilization in dendritic cells, IL-2 activated NK cells, and activated T lymphocytes. A CD8+ T lymphocyte-derived secreted soluble activity that suppresses infection by primary non-syncytium-inducing and syncytium-inducing HIV-1 isolates and the T cell line-adapted isolate HIV-1IIIB, has been identified as CCL22. Based on amino-terminal sequence analysis, the major CD8+ T lymphocyte-derived CCL22 protein yielded an amino-terminal sequence of YGANM, which is two amino acid residues shorter than the predicted mature CCL22. The difference in potency between the two mature CCL22 isoforms has not been determined.

Alternate Names

ABCD-1, MDC, STCP-1

Entrez Gene IDs

6367 (Human); 20299 (Mouse)

Gene Symbol

CCL22

UniProt

O00626

Additional CCL22/MDC Products

Product Documents for Human CCL22/MDC Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CCL22/MDC Antibody

For research use only

Citations for Human CCL22/MDC Antibody

Customer Reviews for Human CCL22/MDC Antibody (1)

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  • Human CCL22/MDC Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 06/09/2020
    We used this antibody in an in-house ELISA along with mAb (336) and protein standard (336-MD) to quantify CCL22 in human serum and plasma. This combination could not detect CCL22 in our samples but generated a good standard curve.
    Human CCL22/MDC Antibody AF336

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