Detects human CCL24/Eotaxin-2/MPIF-2 in ELISAs and Western blots. In sandwich immunoassays, less than 0.03% cross-reactivity with recombinant human (rh) Eotaxin, rhMIP-1 alpha, and rhMCP-3 is observed.
Polyclonal Goat IgG
E. coli-derived recombinant human CCL24/Eotaxin-2/MPIF-2 (R&D Systems, Catalog # 343-E2) Val27-Cys119 Accession # AAB51135
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Recombinant Human CCL24/Eotaxin‑2/MPIF‑2 (Catalog # 343-E2)
Human CCL24/Eotaxin-2/MPIF-2 Sandwich Immunoassay
ELISA Capture (Matched Antibody Pair)
Human CCL24/Eotaxin‑2/MPIF‑2 Antibody (Catalog #
ELISA Detection (Matched Antibody Pair)
Human CCL24/Eotaxin‑2/MPIF‑2 Biotinylated Antibody (Catalog #
Recombinant Human CCL24/Eotaxin-2/MPIF-2 Protein (Catalog #
Please Note: Optimal dilutions should be determined by each laboratory for each application.
are available in the Technical Information section on our website.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Eotaxin, also named MPIF-2 and Ck beta 6, is a novel CC chemokine identified in the Human Genome Sciences, Inc. database based on the presence of the CC motif and homology with other known CC chemokines. Eotaxin-2 cDNA encodes a 119 amino acid residue precursor protein with a 26 aa residue signal peptide that is cleaved to generate a mature protein predicted to contain 93 amino acid residues with an N-glycosylation site. Although one recombinant preparation of MPIF‑2/Eotaxin-2 from insect cells was reported to be glycosylated and of the predicted size (10.5 kDa), a second preparation of recombinant Eotaxin-2 prepared by a different group, also from insect cells, was shown to contain a 78 amino acid residue carboxy-terminally truncated variant of Eotaxin-2. Additional minor carboxy-terminally truncated variants with 73, 75 and 76 residues were also isolated. Compared to other CC chemokines, Eotaxin-2 exhibits 40%, 42%, and 39% amino acid identity to MCP-3, MIP-1 alpha, and Eotaxin, respectively. Eotaxin-2 mRNA is weakly expressed in activated monocytes and T lymphocytes.
Recombinant Eotaxin-2 has been shown to induce chemotaxis of eosinophils, basophils, and resting T lymphocytes but not monocytes and activated T lymphocytes. Eotaxin-2 has also been shown to suppress the colony formation by the high proliferative potential colony-forming cells which represent multipotential hematopoietic progenitors. On eosinophils, the effects of Eotaxin-2 was shown to be inhibited by an anti-CCR-3 antibody and to be cross-desensitized by Eotaxin or MCP-4, suggesting that all three CC chemokines act through CCR3, at least on eosinophils.
Forssmann, U. et al. (1997) J. Exp. Med. 185:2171.
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The data collected includes not only links to publications in PubMed,
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