Recombinant Human CCL24/Eotaxin‑2/MPIF‑2 (Catalog # 343-E2)
Human CCL24/Eotaxin-2/MPIF-2 Sandwich Immunoassay
ELISA Capture (Matched Antibody Pair)
Human CCL24/Eotaxin‑2/MPIF‑2 Antibody (Catalog #
ELISA Detection (Matched Antibody Pair)
Human CCL24/Eotaxin‑2/MPIF‑2 Biotinylated Antibody (Catalog #
Recombinant Human CCL24/Eotaxin-2/MPIF-2 Protein (Catalog #
Measured by its ability to neutralize CCL24/Eotaxin‑2/MPIF‑2-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CCR3. The Neutralization Dose (ND50) is typically 0.2-0.8 µg/mL in the presence of 100 ng/mL Recombinant Human CCL24/Eotaxin‑2/MPIF‑2.
Please Note: Optimal dilutions should be determined by each laboratory for each application.
are available in the Technical Information section on our website.
Chemotaxis Induced by CCL24/Eotaxin‑2 and Neutralization by Human CCL24/Eotaxin‑2 Antibody.
Recombinant Human CCL24/ Eotaxin‑2 (Catalog # 343‑E2) chemoattracts the BaF3 mouse pro‑B cell line transfected with mouse CCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL24/ Eotaxin‑2 (100 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL24/ Eotaxin‑2 Monoclonal Antibody (Catalog # MAB343). The ND50 is typically 0.2‑0.8 µg/mL.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Eotaxin-2, also named MPIF-2 and Ck beta 6, is a CC chemokine that is designated CCL24. Eotaxin-2 cDNA encodes a 10.5 kDa, 119 amino acid residue precursor protein with a 26 aa residue signal peptide that is cleaved to generate a mature protein predicted to contain 93 amino acid residues with an N-glycosylation site. C‑terminally truncated variants with 78, 73, 75 and 76 residues have also been described. Eotaxin-2 shares 40%, 42% and 39% amino acid sequence identity with other CC chemokines CCL7/MCP-3, CCL3/MIP-1 alpha, and CCL11/Eotaxin, respectively. Eotaxin-2 mRNA is weakly expressed in activated monocytes and T lymphocytes. Recombinant Eotaxin-2 induces chemotaxis of eosinophils, basophils, and resting T lymphocytes, but not monocytes or activated T lymphocytes. Eotaxin-2 also suppresses colony formation by high proliferative multipotential hematopoietic progenitors. On eosinophils, the effects of Eotaxin-2, Eotaxin and CCL13/MCP-4 are mediated by the receptor CCR3.
Forssmann, U. et al. (1997) J. Exp. Med. 185:2171.
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