Human CCL24/Eotaxin-2/MPIF-2 Antibody

Catalog # Availability Size / Price Qty
Chemotaxis Induced by CCL24/Eotaxin‑2 and Neutralization by Human CCL24/Eotaxin‑2 Antibody.
1 Image
Product Details
Citations (3)
Supplemental Products

Human CCL24/Eotaxin-2/MPIF-2 Antibody Summary

Species Reactivity
Detects human CCL24/Eotaxin‑2/MPIF‑2 in ELISAs and Western blots. In sandwich ELISAs, less than 0.04% cross-reactivity with recombinant human (rh) Eotaxin, rhMIP-1 alpha, and rhMCP-3 is observed.
Monoclonal Mouse IgG1 Clone # 61016
Protein A or G purified from ascites
E. coli-derived recombinant human CCL24/Eotaxin‑2/MPIF‑2
Accession # AAB51135
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.


Recommended Concentration
Western Blot
1 µg/mL
Recombinant Human CCL24/Eotaxin‑2/MPIF‑2 (Catalog # 343-E2)

Human CCL24/Eotaxin-2/MPIF-2 Sandwich Immunoassay

Recommended Concentration
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human CCL24/Eotaxin‑2/MPIF‑2 Biotinylated Antibody (Catalog # BAF343)

Standard: Recombinant Human CCL24/Eotaxin-2/MPIF-2 Protein (Catalog # 343-E2)

Measured by its ability to neutralize CCL24/Eotaxin‑2/MPIF‑2-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CCR3. The Neutralization Dose (ND50) is typically 0.2-0.8 µg/mL in the presence of 100 ng/mL Recombinant Human CCL24/Eotaxin‑2/MPIF‑2.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Chemotaxis Induced by CCL24/Eotaxin‑2 and Neutralization by Human CCL24/Eotaxin‑2 Antibody. View Larger

Chemotaxis Induced by CCL24/Eotaxin‑2 and Neutralization by Human CCL24/Eotaxin‑2 Antibody. Recombinant Human CCL24/ Eotaxin-2 (Catalog # 343-E2) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL24/ Eotaxin-2 (100 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL24/ Eotaxin-2 Monoclonal Antibody (Catalog # MAB343). The ND50 is typically 0.2-0.8 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL24/Eotaxin-2/MPIF-2

Eotaxin-2, also named MPIF-2 and Ck beta 6, is a CC chemokine that is designated CCL24. Eotaxin-2 cDNA encodes a 10.5 kDa, 119 amino acid residue precursor protein with a 26 aa residue signal peptide that is cleaved to generate a mature protein predicted to contain 93 amino acid residues with an N-glycosylation site. C‑terminally truncated variants with 78, 73, 75 and 76 residues have also been described. Eotaxin-2 shares 40%, 42% and 39% amino acid sequence identity with other CC chemokines CCL7/MCP-3, CCL3/MIP-1 alpha, and CCL11/Eotaxin, respectively. Eotaxin-2 mRNA is weakly expressed in activated monocytes and T lymphocytes. Recombinant Eotaxin-2 induces chemotaxis of eosinophils, basophils, and resting T lymphocytes, but not monocytes or activated T lymphocytes. Eotaxin-2 also suppresses colony formation by high proliferative multipotential hematopoietic progenitors. On eosinophils, the effects of Eotaxin-2, Eotaxin and CCL13/MCP-4 are mediated by the receptor CCR3.

  1. Forssmann, U. et al. (1997) J. Exp. Med. 185:2171.
  2. Patel, V.P. et al. (1997) J. Exp. Med. 185:1163.
Entrez Gene IDs
6369 (Human); 56221 (Mouse)
Alternate Names
CCL24; chemokine (C-C motif) ligand 24; Ckb-6; Eosinophil chemotactic protein 2; Eotaxin-2; member 24; MPIF2; MPIF-2; MPIF2Myeloid progenitor inhibitory factor 2; MPIF-2Small-inducible cytokine A24; SCYA24CK-beta-6

Product Datasheets

You must select a language.


Citations for Human CCL24/Eotaxin-2/MPIF-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
Filter your results:

Filter by:

  1. Eotaxin-2 and colorectal cancer: a potential target for immune therapy.
    Authors: Cheadle EJ, Riyad K, Subar D, Rothwell DG, Ashton G, Batha H, Sherlock DJ, Hawkins RE, Gilham DE
    Clin. Cancer Res., 2007-10-01;13(19):5719-28.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: ELISA Development
  2. A pharmacological chaperone improves memory by reducing A&beta and tau neuropathology in a mouse model with plaques and tangles
    Authors: JG Li, J Chiu, M Ramanjulu, BE Blass, D Praticò
    Mol Neurodegener, 2020-01-22;15(1):1.
  3. Assessment of folate receptor alpha and beta expression in selection of lung and pancreatic cancer patients for receptor targeted therapies
    Authors: Jiayin Shen, Yingwen Hu, Karson S. Putt, Sunil Singhal, Haiyong Han, Daniel W. Visscher et al.


No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human CCL24/Eotaxin-2/MPIF-2 Antibody

There are currently no reviews for this product. Be the first to review Human CCL24/Eotaxin-2/MPIF-2 Antibody and earn rewards!

Have you used Human CCL24/Eotaxin-2/MPIF-2 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review