Human CCL24/Eotaxin-2/MPIF-2 Antibody Summary
Accession # AAB51135
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
CCL24/Eotaxin‑2/MPIF‑2 in Human Skin. CCL24/Eotaxin-2/MPIF-2 was detected in immersion fixed paraffin-embedded sections of human skin using 15 µg/mL Goat Anti-Human CCL24/Eotaxin-2/ MPIF-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF343) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Chemotaxis Induced by CCL24/Eotaxin‑2 and Neutralization by Human CCL24/Eotaxin‑2 Antibody. Recombinant Human CCL24/Eotaxin-2 (Catalog # 343-E2) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL24/Eotaxin-2 (0.1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL24/Eotaxin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF343). The ND50 is typically 0.5-2.5 µg/mL.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Eotaxin, also named MPIF-2 and Ck beta 6, is a novel CC chemokine identified in the Human Genome Sciences, Inc. database based on the presence of the CC motif and homology with other known CC chemokines. Eotaxin-2 cDNA encodes a 119 amino acid residue precursor protein with a 26 aa residue signal peptide that is cleaved to generate a mature protein predicted to contain 93 amino acid residues with an N-glycosylation site. Although one recombinant preparation of MPIF‑2/Eotaxin‑2 from insect cells was reported to be glycosylated and of the predicted size (10.5 kDa), a second preparation of recombinant Eotaxin-2 prepared by a different group, also from insect cells, was shown to contain a 78 amino acid residue carboxy-terminally truncated variant of Eotaxin-2. Additional minor carboxy‑terminally truncated variants with 73, 75 and 76 residues were also isolated. Compared to other CC chemokines, Eotaxin-2 exhibits 40%, 42% and 39% amino acid identity to MCP-3, MIP-1 alpha, and Eotaxin, respectively. Eotaxin-2 mRNA is weakly expressed in activated monocytes and T lymphocytes.
Recombinant Eotaxin-2 has been shown to induce chemotaxis of eosinophils, basophils, and resting T lymphocytes but not monocytes and activated T lymphocytes. Eotaxin-2 has also been shown to suppress the colony formation by the high proliferative potential colony-forming cells which represent multipotential hematopoietic progenitors. On eosinophils, the effects of Eotaxin-2 was shown to be inhibited by an anti-CCR-3 antibody and to be cross-desensitized by Eotaxin or MCP-4, suggesting that all three CC chemokines act through CCR-3, at least on eosinophils.
- Forssmann, U. et al. (1997) J. Exp. Med. 185:2171.
- Patel, V.P. et al. (1997) J. Exp. Med. 185:1163.
Citations for Human CCL24/Eotaxin-2/MPIF-2 Antibody
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Mast cell beta-tryptase selectively cleaves eotaxin and RANTES and abrogates their eosinophil chemotactic activities.
Authors: Pang L, Nie M, Corbett L, Sutcliffe A, Knox AJ
J. Immunol., 2006;176(6):3788-95.
Sample Types: Recombinant Protein
Applications: Western Blot
An initial investigation into endothelial CC chemokine expression in the human rheumatoid synovium.
Authors: Rump L, Mattey D, Kehoe O, Middleton J
Sample Types: Whole Tissue
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