Human CCL26/Eotaxin-3 Antibody [Biotin]

R&D Systems | Catalog # BAF346

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Primate - Macaca mulatta (Rhesus Macaque)

Applications

Validated:

Western Blot, ELISA Detection (Matched Antibody Pair)

Cited:

Immunohistochemistry, ELISA Development

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all CCL26/Eotaxin-3 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CCL26/Eotaxin-3 (R&D Systems, Catalog # 346-E3)
Thr24-Leu94
Accession # Q9Y258

Specificity

Detects human CCL26/Eotaxin-3 in ELISAs and Western blots. In sandwich immunoassays, less than 0.5% cross-reactivity with recombinant human (rh) Eotaxin, rhEotaxin‑2, recombinant mouse (rm) Eotaxin, and rmEotaxin‑2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human CCL26/Eotaxin-3 Antibody [Biotin]

Human CCL26 / Eotaxin-3 ELISA Standard Curve

Human CCL26 / Eotaxin-3 ELISA Standard Curve

Recombinant Human CCL26/Eotaxin‑3 (Catalog # 653-E3) was serially diluted and captured by Mouse Anti-Human CCL26/Eotaxin‑3 Monoclonal Antibody (Catalog # MAB653) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human BMPR‑IA/ALK‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF346) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human CCL26/Eotaxin-3 Antibody [Biotin]

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human CCL26/Eotaxin‑3 (Catalog # 346-E3)

Human CCL26/Eotaxin-3 Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Human CCL26/Eotaxin‑3 Antibody (Catalog # MAB653)
  • Standard: Recombinant Human CCL26/Eotaxin-3 Protein (Catalog # 653-E3)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using BAF346 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: CCL26/Eotaxin-3

Eotaxin-3, also named CCL26 or SCYA26, is a novel human CC chemokine that maps to chromosome 7q11.2, within 40 kilobases of the Eotaxin-2 loci. Eotaxin-3/CCL26 has been shown to be constitutively expressed in the heart and ovary. In addition, low levels of Eotaxin-3/CCL26 expression can also be detected in various tissues. The expression of Eotaxin-3/CCL26 in vascular endothelial cells has been shown to be up-regulated by IL-13 and IL-4. Eotaxin-3/CCL26 cDNA encodes a 94 amino acid (aa) residue protein with a putative signal peptide of either 23 or 26 aa residues. Eotaxin-3/CCL26 induces calcium flux in eosinophils as well as in CCR3-transfected cells. Eotaxin-3/CCL26 has also been shown to cross-desensitize cells to other CCR3 ligands.

References

  1. Gou, R.F. et al. (1999) Genomics 58:313.
  2. Kitamura, M. et al. (1999) J. Biol. Chem. 274:27975.
  3. Shinkai, A. et al. (1999) J. Immunol. 163:1602.

Alternate Names

Eotaxin-3, Eotaxin3, SCYA26

Entrez Gene IDs

10344 (Human)

Gene Symbol

CCL26

UniProt

Q9Y258

Additional CCL26/Eotaxin-3 Products

Product Documents for Human CCL26/Eotaxin-3 Antibody [Biotin]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human CCL26/Eotaxin-3 Antibody [Biotin]

For research use only

Citations for Human CCL26/Eotaxin-3 Antibody [Biotin]

Customer Reviews for Human CCL26/Eotaxin-3 Antibody [Biotin] (1)

5 out of 5
1 Customer Rating
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Human CCL26/Eotaxin-3 Antibody [Biotin]?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 for a review with an image

$10/€7/£6/$10CAN/¥1110 for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • Human CCL26/Eotaxin‑3 Biotinylated Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 07/19/2019
    This antibody was used to detected in an ELISA human CCL26 in human serum samples.
    Human CCL26/Eotaxin-3 Antibody [Biotin] BAF346

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies