Human CCL26/Eotaxin-3 Antibody Summary
Accession # Q9Y258
Human CCL26/Eotaxin-3 Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human CCL26/Eotaxin‑3 by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells untreated (-) or treated (+) with 100 U/mL Recombinant Human IL-4 (Catalog # 204-IL) for 24 hours. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human CCL26/Eotaxin-3 Monoclonal Antibody (Catalog # MAB653) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CCL26/ Eotaxin-3 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Chemotaxis Induced by CCL26/Eotaxin‑3 and Neutralization by Human CCL26/Eotaxin‑3 Antibody. Recombinant Human CCL26/Eotaxin-3 (Catalog # 346-E3) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL26/Eotaxin-3 (1 µg/mL) is neutralized (green line) by increasing concentrations of Human CCL26/Eotaxin-3 Monoclonal Antibody (Catalog # MAB653). The ND50is typically 2-8 µg/mL.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
CCL26, also known as Eotaxin-3, is a CC chemokine that functions as a chemoattractant for eosinophils and activated T cells through interactions with CCR3.
Citations for Human CCL26/Eotaxin-3 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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Eotaxins and CCR3 interaction regulates the Th2 environment of cutaneous T-cell lymphoma.
Authors: Miyagaki T, Sugaya M, Fujita H
J. Invest. Dermatol., 2010;130(9):2304-11.
Sample Types: Whole Tissue
Rapid chemokine secretion from endothelial cells originates from 2 distinct compartments.
Authors: Oynebraten I, Bakke O, Brandtzaeg P, Johansen FE, Haraldsen G
Sample Types: Cell Culture Supernates
Applications: ELISA Development
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