Human CCL26/Eotaxin-3 Antibody

(2 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human CCL26/Eotaxin-3 in ELISAs and Western blots. Does not cross-react with recombinant human (rh) CCL11/Eotaxin, recombinant mouse (rm) CCL11/Eotaxin, rhCCL24/Eotaxin-2, rmCCL24/Eotaxin-2, rhCCL3, rhCCL4, rmCCL3, rmCCL4, rhCCL5, rhCCL13, or rhCCL14.
  • Source
    Monoclonal Mouse IgG1 Clone # 115002
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    E. coli-derived recombinant human CCL26/Eotaxin-3
    Thr24-Leu94
    Accession # Q9Y258
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
    • Human CCL26/Eotaxin-3 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Human CCL26/Eotaxin‑3 Antibody (Catalog # MAB653)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Human CCL26/Eotaxin‑3 Biotinylated Antibody (Catalog # BAF346)
  • ELISA Standard
     
    Recombinant Human CCL26/Eotaxin-3 Protein (Catalog # 653-E3)
  • Neutralization
    Measured by its ability to neutralize CCL26/Eotaxin‑3-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CCR3. The Neutralization Dose (ND50) is typically 2-8 µg/mL in the presence of 1 µg/mL Recombinant Human CCL26/Eotaxin‑3.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human CCL26/Eotaxin‑3 by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells untreated (-) or treated (+) with
100 U/mL Recombinant Human IL‑4 (Catalog # 204-IL) for 24 hours. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human CCL26/Eotaxin‑3 Monoclonal Antibody (Catalog # MAB653) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CCL26/
Eotaxin‑3 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Chemotaxis Induced by CCL26/Eotaxin‑3 and Neutralization by Human CCL26/Eotaxin‑3 Antibody. Recombinant Human CCL26/Eotaxin‑3 (Catalog # 346‑E3) chemoattracts the BaF3 mouse pro‑B cell line transfected with mouse CCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL26/Eotaxin‑3 (1 µg/mL) is neutralized (green line) by increasing concentrations of Human CCL26/Eotaxin‑3 Monoclonal Antibody (Catalog # MAB653). The ND50 is typically 2-8 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL26/Eotaxin-3
CCL26, also known as Eotaxin-3, is a CC chemokine that functions as a chemoattractant for eosinophils and activated T cells through interactions with CCR3.
  • Entrez Gene IDs:
    10344 (Human)
  • Alternate Names:
    CC chemokine IMAC; C-C motif chemokine 26; CCL26; chemokine (C-C motif) ligand 26; chemokine N1; Eotaxin3; Eotaxin-3; IMAC; Macrophage inflammatory protein 4-alpha; MGC126714; MIP-4a; MIP-4alpha; SCYA26; SCYA26MIP-4-alpha; small inducible cytokine A26; small inducible cytokine subfamily A (Cys-Cys), member 26; Small-inducible cytokine A26; TSC-1Thymic stroma chemokine-1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. Eotaxins and CCR3 interaction regulates the Th2 environment of cutaneous T-cell lymphoma.
    Authors: Miyagaki T, Sugaya M, Fujita H
    J. Invest. Dermatol., 2010;130(9):2304-11.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  2. Rapid chemokine secretion from endothelial cells originates from 2 distinct compartments.
    Authors: Oynebraten I, Bakke O, Brandtzaeg P, Johansen FE, Haraldsen G
    Blood, 2004;104(2):314-20.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Isotype Control

Ctrl MAB002 138  
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Mouse IgG1 Isotype Control

Ctrl MAB002R 1
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse IgG Antibody

WB AF007 4
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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