Detection of CCR7 in Human Blood Lymphocytes by Flow Cytometry.
Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD4 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB3791F) and either (A) Mouse Anti-Human CCR7 PerCP‑conjugated Monoclonal Antibody (Catalog # FAB197C) or (B) Mouse IgG2A PerCP Isotype Control (Catalog # IC003C). View our protocol for Staining Membrane-associated Proteins.
Detection of CCR7 in HEK293 Human Cell Line Transfected with Human CCR7 and eGFP by Flow Cytometry.
The specificity of Mouse Anti-Human CCR7 Clone 150503 was demonstrated by its ability to react with (A) HEK293 human embryonic kidney cell line transfected with human CCR7 and not react with (B) irrelevant transfectants (HEK293 human embryonic kidney cell line transfected with human CCR7 and eGFP). Antibody binding was monitored using Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
CCR7 is a 7 transmembrane G protein coupled chemokine receptor. CCR7 is expressed on T cells and mature dendritic cells and transduces chemotactic signals in response to CCL19 and CCL21. Human CCR7 shares 87% amino acid sequence identity with mouse CCR7.
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