Human CD109 Antibody

Catalog # Availability Size / Price Qty
AF4385
AF4385-SP
Human CD109 Antibody in Flow Cytometry
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Human CD109 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD109 in direct ELISAs and Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD109
Val22-Ser1268 (Tyr703Ser, Thr1241Met)
Accession # Q6YHK3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human CD109 (Catalog # 4385-CD)
Flow Cytometry
2.5 µg/106 cells
See below
Immunohistochemistry
5-15 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of CD109 in A431 Human Cell Line by Flow Cytometry. View Larger

Detection of CD109 in A431 Human Cell Line by Flow Cytometry. A431 human epithelial carcinoma cell line was stained with Human CD109 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF4385, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL010).

Immunohistochemistry CD109 in Human Squamous Cell Carcinoma. View Larger

CD109 in Human Squamous Cell Carcinoma. CD109 was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using 15 µg/mL Sheep Anti-Human CD109 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4385) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD109

CD109 is a GPI-anchored member of the alpha-2-macroglobulin (A2M) and complement family of proteins (1). Mature human CD109 contains a bait region with recognition sequences for multiple proteases, an internal thioester bond, and a domain similar to the receptor binding domain of A2M (2). Cleavage of A2M family proteins within the bait region activates the thioester bond to promote covalent bonding to nucleophilic groups in adjacent molecules (3, 4). Within the region included in this recombinant protein, human CD109 shares 71-73% amino acid (aa) sequence identity with mouse and rat CD109. It shares 27-33% aa sequence identity with A2M and complement factors C3, C4, and C5. Alternate splicing of human CD109 generates two isoforms with short deletions and one that is truncated within the bait region. CD109 is expressed on activated T cells and platelets, hematopoietic stem cells, megakaryocyte precursors, vascular endothelial cells, basal and myoepithelial cells of secretory glands, and squamous cell carcinomas (2, 5-9). It is produced as a 170-180 kDa glycoprotein that is autocatalytically processed to 150 kDa and 120 kDa forms (2, 6, 10). CD109 on keratinocytes binds TGF-beta and associates with TGF-beta RI and TGF-beta RII, resulting in inhibition of TGF-beta signaling (11). Polymorphisms of CD109 include the platelet-specific Gov antigen and the blood group ABH antigens (12, 13). Alloantibodies directed against these antigens result in unsuccessful platelet transfusions, neonatal alloimmune thrombocytopenia, and posttransfusion purpura (14).

References
  1. Travis, J. and G.S. Salvesen (1983) Annu. Rev. Biochem. 52:655.
  2. Lin, M. et al. (2002) Blood 99:1683.
  3. Christensen, U. and L. Sottrup-Jensen (1984) Biochemistry 23:6619.
  4. Wallis, R. et al. (2007) J. Biol. Chem. 282:7844.
  5. Murray, L.J. et al. (1999) Exp. Hematol. 27:1282.
  6. Haregewoin, A. et al. (1994) Cell. Immunol. 156:357.
  7. Hasegawa, M. et al. (2007) Pathol. Int. 57:245.
  8. Brashem-Stein, C. et al. (1988) J. Immunol. 140:2330.
  9. Hashimoto, M. et al. (2004) Oncogene 23:3716.
  10. Solomon, K.R. et al. (2004) Gene 327:171.
  11. Finnson, K.W. et al. (2006) FASEB J. 20:1525.
  12. Schuh, A.C. et al. (2002) Blood 99:1692.
  13. Kelton, J.G. et al. (1998) J. Lab. Clin. Med. 132:142.
  14. Rozman, P. (2002) Transpl. Immunol. 10:165.
Entrez Gene IDs
135228 (Human); 235505 (Mouse); 363104 (Rat)
Alternate Names
150 kDa TGF-beta-1-binding protein; activated T-cell marker CD109; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 7; CD109 antigen (Gov platelet alloantigens); CD109 antigen; CD109 molecule; CD109; CPAMD7; CPAMD7r150; DKFZp762L1111; FLJ38569; FLJ41966; Gov platelet alloantigens; p180; Platelet-specific Gov antigen; RP11-525G3.1

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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Human CD109 Antibody
By Anonymous on 11/29/2016
Application: Flow Sample Tested: HUVEC human umbilical vein endothelial cells Species: Human