Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, Flow Cytometry, Simple Western, CyTOF-ready

Cited:

Immunohistochemistry, Western Blot, Immunocytochemistry, Co-Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all CD109 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CD109
Val22-Ser1268 (Tyr703Ser, Thr1241Met)
Accession # Q6YHK3

Specificity

Detects human CD109 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human CD109 Antibody

Detection of Human CD109 by Western Blot.

Western blot shows lysates of U‑87 MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human CD109 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4385) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for CD109 at approximately 161 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of CD109 antibody in A431 Human Cell Line antibody by Flow Cytometry.

Detection of CD109 in A431 Human Cell Line by Flow Cytometry.

A431 human epithelial carcinoma cell line was stained with Human CD109 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF4385, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL010).

CD109 antibody in Human Squamous Cell Carcinoma by Immunohistochemistry (IHC-P).

CD109 in Human Squamous Cell Carcinoma.

CD109 was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using 15 µg/mL Sheep Anti-Human CD109 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4385) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human CD109 by Simple WesternTM.

Simple Western lane view shows lysates of U‑87 MG human glioblastoma/astrocytoma cell line, loaded at 0.2 mg/mL. A specific band was detected for CD109 at approximately 161 kDa (as indicated) using 20 µg/mL of Sheep Anti-Human CD109 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4385). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human CD109 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: A431 human epithelial carcinoma cell line

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human squamous cell carcinoma

Simple Western

20 µg/mL
Sample: U‑87 MG human glioblastoma/astrocytoma cell line

Western Blot

0.5 µg/mL
Sample: U‑87 MG human glioblastoma/astrocytoma cell line

Reviewed Applications

Read 1 review rated 4 using AF4385 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD109

CD109 is a GPI-anchored member of the alpha-2-macroglobulin (A2M) and complement family of proteins (1). Mature human CD109 contains a bait region with recognition sequences for multiple proteases, an internal thioester bond, and a domain similar to the receptor binding domain of A2M (2). Cleavage of A2M family proteins within the bait region activates the thioester bond to promote covalent bonding to nucleophilic groups in adjacent molecules (3, 4). Within the region included in this recombinant protein, human CD109 shares 71-73% amino acid (aa) sequence identity with mouse and rat CD109. It shares 27-33% aa sequence identity with A2M and complement factors C3, C4, and C5. Alternate splicing of human CD109 generates two isoforms with short deletions and one that is truncated within the bait region. CD109 is expressed on activated T cells and platelets, hematopoietic stem cells, megakaryocyte precursors, vascular endothelial cells, basal and myoepithelial cells of secretory glands, and squamous cell carcinomas (2, 5-9). It is produced as a 170-180 kDa glycoprotein that is autocatalytically processed to 150 kDa and 120 kDa forms (2, 6, 10). CD109 on keratinocytes binds TGF-beta and associates with TGF-beta RI and TGF-beta RII, resulting in inhibition of TGF-beta signaling (11). Polymorphisms of CD109 include the platelet-specific Gov antigen and the blood group ABH antigens (12, 13). Alloantibodies directed against these antigens result in unsuccessful platelet transfusions, neonatal alloimmune thrombocytopenia, and posttransfusion purpura (14).

References

  1. Travis, J. and G.S. Salvesen (1983) Annu. Rev. Biochem. 52:655.
  2. Lin, M. et al. (2002) Blood 99:1683.
  3. Christensen, U. and L. Sottrup-Jensen (1984) Biochemistry 23:6619.
  4. Wallis, R. et al. (2007) J. Biol. Chem. 282:7844.
  5. Murray, L.J. et al. (1999) Exp. Hematol. 27:1282.
  6. Haregewoin, A. et al. (1994) Cell. Immunol. 156:357.
  7. Hasegawa, M. et al. (2007) Pathol. Int. 57:245.
  8. Brashem-Stein, C. et al. (1988) J. Immunol. 140:2330.
  9. Hashimoto, M. et al. (2004) Oncogene 23:3716.
  10. Solomon, K.R. et al. (2004) Gene 327:171.
  11. Finnson, K.W. et al. (2006) FASEB J. 20:1525.
  12. Schuh, A.C. et al. (2002) Blood 99:1692.
  13. Kelton, J.G. et al. (1998) J. Lab. Clin. Med. 132:142.
  14. Rozman, P. (2002) Transpl. Immunol. 10:165.

Alternate Names

CD109, CPAMD7

Entrez Gene IDs

135228 (Human); 235505 (Mouse); 363104 (Rat)

Gene Symbol

CD109

UniProt

Q6YHK3

Additional CD109 Products

Product Documents for Human CD109 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CD109 Antibody

For research use only

Citations for Human CD109 Antibody

Customer Reviews for Human CD109 Antibody (1)

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  • Human CD109 Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: HUVEC human umbilical vein endothelial cells
    Species: Human
    Verified Customer | Posted 11/29/2016
    Human CD109 Antibody AF4385

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Protocols

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