Human CD14 Antibody

Name: Human CD14 Antibody
Brand: R&D Systems
SKU: AB383

R&D Systems | Catalog # AB383

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Citations (2)

Product Documents

Discontinued Product

AB383 has been discontinued. An alternative/replacement product is available: AF3831. View all CD14 products.

Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Western Blot, Neutralization, Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all CD14 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human CD14

Specificity

Detects human CD14 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant mouse CD14 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human CD14 Antibody

Detection of Human CD14 by Western Blot.

Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nM of PMA for 24 hours and 10 ug/mL of LPS for 3 hours. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human CD14 Polyclonal Antibody (Catalog # AB383) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for CD14 at approximately 55 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Western Blot Buffer Group 1.

Detection of CD14 in Human Liver.

CD14 was detected in immersion fixed paraffin-embedded sections of Human Liver using Sheep Anti-Human CD14 Polyclonal Antibody (Catalog # AB383) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Sheep IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC006). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to sinusoids. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of CD14 in Human Tonsil.

CD14 was detected in immersion fixed paraffin-embedded sections of Human Tonsil using Sheep Anti-Human CD14 Polyclonal Antibody (Catalog # AB383) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Sheep IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC006). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes in germinal center. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human CD14 Antibody

Application

Recommended Usage

Immunohistochemistry

3-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of Human Liver and Human Tonsil

Western Blot

1 µg/mL
Sample: THP‑1 human acute monocytic leukemia cell line treated with PMA and LPS

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: CD14

CD14 is a 55 kDa cell surface glycoprotein that is preferentially expressed on monocytes/macrophages. The human CD14 cDNA encodes a 375 amino acid (aa) residue precursor protein with a 19 aa signal peptide and a C-terminal hydrophobic region characteristic for glycosylphosphatidyinositol (GPI)-anchored proteins. Human CD14 has four potential N-linked glycosylation sites and also bears O-linked carbohydrates. The amino acid sequence of human CD14 is approximately 65% identical with the mouse, rat, rabbit, and bovine proteins. CD14 is a pattern recognition receptor that binds lipopolysaccharides (LPS) and a variety of ligands derived from different microbial sources. The binding of CD14 with LPS is catalyzed by LPS-binding protein (LBP). The toll-like-receptors have also been implicated in the transduction of CD14-LPS signals. Similar to other GPI-anchored proteins, soluble CD14 can be released from the cell surface by phosphatidyinositol-specific phospholipase C. Soluble CD14 has been detected in serum and body fluids. High concentrations of soluble CD14 have been shown to inhibit LPS-mediated responses. However, soluble CD14 can also potentiate LPS response in cells that do not express cell surface CD14.