Detection of Human CD160 by Western Blot. Western blot shows lysates of human small intestine tissue. PVDF Membrane was probed with 1 µg/mL of Human CD160 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6177) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD160 at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
Sterile PBS to a final concentration of 0.2 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
CD160 (also BY55) is a 27 kDa member of Ig Superfamily of molecules. It is expressed on select hematopoietic cell types, including CD56dim CD16+ cytotoxic NK cells, CD8+ CD28- effector T cells, gamma δ T cells, and restricted CD4+ T cells. It is a receptor for HLA-C molecules, and its engagement induces CD160+ NK cells to both secrete IFN-gamma plus TNF-alpha and initiate a cytotoxic program. Human CD160 was originally identified as a 155 amino acid (aa) proprotein (aa 27-181) (SwissProt #:O95971). It contains a 132 aa mature region (aa 27-159) and a C-terminal prosegment that is cleaved to create a GPI linkage. The mature region possesses one V‑type Ig-like domain (aa 27-122). CD160 is found as a soluble, disulfide-linked 80 kDa multimer (likely trimer) that is generated by proteolysis of the GPI-linked form. This 80 kDa form, plus others, are highly resistant to reduction. There is also a 100-110 kDa multimeric transmembrane (TM) form that is associated with activated NK cells. It contains a 55 aa substitution for Gly180Leu181, and shows a 20 aa TM segment between aa 163-182. The TM form appears to have a splice variant that lacks aa 25-133. Over aa 27-159, human CD160 shares only 62% aa sequence identity with mouse CD160.
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