Human CD19 Biotinylated Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human CD19 in flow cytometry.
  • Source
    Monoclonal Mouse IgG1 Clone # 4G7-2E3
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Human CLL cells
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Biotin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CD19 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD3 epsilon APC‑conjugated Monoclonal Antibody (Catalog # FAB100A) and either (A) Mouse Anti-Human CD19 Biotinylated Monoclonal Antibody (Catalog # FAB4867B) or (B) Mouse IgG1 Biotinylated Isotype Control (Catalog # IC002B) followed by Streptavidin-Phycoerythrin (Catalog # F0040). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD19

CD19 is a 95 kDa transmembrane glycoprotein with two Ig-like C2-set domains. CD19 regulates B cell development and activation through interactions with CD21, CD22, and the B cell receptor. CD19 polymorphisms and up‑regulation lead to the development of autoimmunity by promoting autoantibody production. Within the extracellular domain, human CD19 (Accession # P15391) shares 57% amino acid sequence identity with mouse and rat CD19.

  • Entrez Gene IDs:
    930 (Human); 12478 (Mouse); 365367 (Rat); 607898 (Canine); 100147468 (Equine)
  • Alternate Names:
    B4; B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; CD19 antigen; CD19 molecule; CD19; CVID3; Differentiation antigen CD19; Leu-12; MGC12802; T-cell surface antigen Leu-12
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Biotinylated Antibody

Ctrl IC002B 2
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Streptavidin-Phycoerythrin

Flow F0040 6
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Streptavidin-Alkaline Phosphatase

ELISA(Det) AR001 1
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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NorthernLights Streptavidin NL557

Flow, IHC, ICC NL999 4
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NorthernLights Streptavidin NL637

Flow, IHC, ICC NL998 2
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NorthernLights Streptavidin NL493

Flow, IHC, ICC NL997 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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Streptavidin-Allophycocyanin

Flow F0050 1
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Avidin-Fluorescein

Flow F0030 1
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Supplemental Cell Selection Products
Description Application Cat# Citations Images  

MagCellect Streptavidin Ferrofluid

MAG999B 4
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MagCellect Streptavidin Ferrofluid

MAG999 4
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Supplemental ELISA Products
Description Application Cat# Citations Images  

Streptavidin-HRP (set of 5 vials enough for 75 plates total)

ELISA DY998 43
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EvenCoat Streptavidin Coated Plates, 15 Pack

ELISA CP004
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EvenCoat Streptavidin Coated Plates, 5 Pack

ELISA CP003 3
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