Detection of CD277/BTN3A1 in Human Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD277/BTN3A1 Monoclonal Antibody (Catalog # MAB7136, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Butyrophilin 3A1 (BTN3A1), also known as CD277, has high structural homology to the B7 superfamily of proteins and is expressed in various immune cells such as T and NK cells. BTN3A1 enhances TCR-induced cytokine production and cell proliferation. Early T-cell activation events such as TCR-induced cell signaling are increased upon BTN3A1 engagement. The BTN3A1 co-stimulatory pathway may be involved in the regulation of various cell-mediated immune responses.
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