Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Human IgG1 Clone # Hu113
View all CD3 Primary Antibodies »

Product Specifications

Immunogen

Human CD3

Specificity

Detects Human CD3 based on Teplizumab therapeutic antibody. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Teplizumab. This product is for research use only.

Clonality

Monoclonal

Host

Human

Isotype

IgG1

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human CD3 (Research Grade Teplizumab Biosimilar) Antibody

Detection of CD3 antibody in Human PBMCs antibody by Flow Cytometry.

Detection of CD3 in Human PBMCs by Flow Cytometry.

Human PBMCs were stained with (A) Human CD3 (Teplizumab Biosimilar) Monoclonal Antibody (Catalog # MAB9929) or (B) no primary antibody, followed by APC-conjugated Anti-Human IgG Secondary Antibody (Catalog # F0135) and Mouse anti-Human CD3 PE-conjugated Monoclonal Antibody (Catalog # FAB100P). View our protocol for Staining Membrane-associated Proteins.
Detection of CD3 antibody in Human Jurkat cell line antibody by Flow Cytometry.

Detection of CD3 in Human Jurkat cell line by Flow Cytometry.

Human Jurkat cell line was stained with Human CD3 (Teplizumab Biosimilar) Monoclonal Antibody (Catalog # MAB9929, filled histogram) or Normal Human IgG control antibody (Catalog # 1-001-A, open histogram) followed by APC-conjugated Anti-Human IgG Secondary Antibody (Catalog # F0135). View our protocol for Staining Membrane-associated Proteins.

Applications for Human CD3 (Research Grade Teplizumab Biosimilar) Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Human PBMCs and Jurkat cell line

Reviewed Applications

Read 1 review rated 5 using MAB9929 in the following applications:

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C, as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 to -70 °C under sterile conditions after opening.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: CD3

Teplizumab is a biosimilar directed against the extracellular domain of CD3. This antibody can be used in flow cytometry or various assay format to measure CD3 expression.

Alternate Names

CD_antigen: CD3e, CD3 antigen, delta subunit, CD3d antigen, CD3d antigen, delta polypeptide (TiT3 complex), CD3d molecule, delta (CD3-TCR complex), CD3-DELTA, CD3e, CD3e antigen, CD3e antigen, epsilon polypeptide (TiT3 complex), CD3e molecule, epsilon (CD3-TCR complex), CD3-epsilon, CD3G, CD3g antigen, CD3g antigen, gamma polypeptide (TiT3 complex), CD3g molecule, epsilon (CD3-TCR complex), CD3g molecule, gamma (CD3-TCR complex), CD3-GAMMA, FLJ17620, FLJ17664, FLJ18683, FLJ79544, FLJ94613, IMD18, MGC138597, T3DOKT3, delta chain, T3E, T-cell antigen receptor complex, epsilon subunit of T3, T-cell receptor T3 delta chain, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 delta chain, T-cell surface glycoprotein CD3 epsilon chain, TCRE

Entrez Gene IDs

916 (Human); 12501 (Mouse)

Gene Symbol

CD3E

Additional CD3 Products

Product Documents for Human CD3 (Research Grade Teplizumab Biosimilar) Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human CD3 (Research Grade Teplizumab Biosimilar) Antibody

For research use only

Customer Reviews for Human CD3 (Research Grade Teplizumab Biosimilar) Antibody (1)

5 out of 5
1 Customer Rating
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Human CD3 (Research Grade Teplizumab Biosimilar) Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • Human CD3 (Research Grade Teplizumab Biosimilar) Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Human recombinant antibody and Jurkat human acute T cell leukemia cell line
    Species: Human
    Verified Customer | Posted 12/12/2024
    Human CD3 (Research Grade Teplizumab Biosimilar) Antibody MAB9929

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

FAQs for Human CD3 (Research Grade Teplizumab Biosimilar) Antibody

Showing  1 - 3 of 3 FAQs Showing All

  • Q: Does this Biosimilar antibody have the same sequence as the therapeutic antibody?

    A: The Biosimilar antibody has the same variable region and constant region sequence as the therapeutic antibody, except for Catalog numbers MAB9677, MAB9575, MAB9577, MAB9589, and their conjugates. These four antibodies have the same variable region as the Biosimilar, but not the same constant region.

  • Q: What is a Biosimilar antibody?

    A: A Biosimilar antibody is a non-therpeutic, recombinantly-expressed antibody which has the same sequence as the corresponding therapeutic antibody.  It is for research use only. The nomenclature of the Biosimilar antibody includes the name of the therapeutic antibody.  For example, Catalog # MAB9577 is called Human EGFR (Research Grade CetuxiMab Biosimilar) antibody.

  • Q: What is the light chain of Human CD3 (Research Grade Teplizumab Biosimilar) Antibody, Catalog # MAB9929? 

    A: The light chain is kappa. 

  • Q: Does this Biosimilar antibody have the same sequence as the therapeutic antibody?

    A: The Biosimilar antibody has the same variable region and constant region sequence as the therapeutic antibody, except for Catalog numbers MAB9677, MAB9575, MAB9577, MAB9589, and their conjugates. These four antibodies have the same variable region as the Biosimilar, but not the same constant region.

  • Q: What is a Biosimilar antibody?

    A: A Biosimilar antibody is a non-therpeutic, recombinantly-expressed antibody which has the same sequence as the corresponding therapeutic antibody.  It is for research use only. The nomenclature of the Biosimilar antibody includes the name of the therapeutic antibody.  For example, Catalog # MAB9577 is called Human EGFR (Research Grade CetuxiMab Biosimilar) antibody.

  • Q: What is the light chain of Human CD3 (Research Grade Teplizumab Biosimilar) Antibody, Catalog # MAB9929? 

    A: The light chain is kappa. 

  • Q: Does this Biosimilar antibody have the same sequence as the therapeutic antibody?

    A: The Biosimilar antibody has the same variable region and constant region sequence as the therapeutic antibody, except for Catalog numbers MAB9677, MAB9575, MAB9577, MAB9589, and their conjugates. These four antibodies have the same variable region as the Biosimilar, but not the same constant region.

  • Q: What is a Biosimilar antibody?

    A: A Biosimilar antibody is a non-therpeutic, recombinantly-expressed antibody which has the same sequence as the corresponding therapeutic antibody.  It is for research use only. The nomenclature of the Biosimilar antibody includes the name of the therapeutic antibody.  For example, Catalog # MAB9577 is called Human EGFR (Research Grade CetuxiMab Biosimilar) antibody.

  • Q: What is the light chain of Human CD3 (Research Grade Teplizumab Biosimilar) Antibody, Catalog # MAB9929? 

    A: The light chain is kappa. 

Showing  1 - 3 of 3 FAQs Showing All
View all FAQs for Antibodies