Human CDO Antibody

Catalog # Availability Size / Price Qty
AF4384
AF4384-SP
Detection of CDO in C2C12 Mouse Cell Line by Flow Cytometry.
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Product Details
Citations (1)
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Human CDO Antibody Summary

Species Reactivity
Human
Specificity
Detects human CDO in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross-reactivity with recombinant mouse CDO is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CDO
Asp26-Pro943 (Leu669Ile)
Accession # NP_058648
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human CDO (Catalog # 4384-CD)
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of CDO in C2C12 Mouse Cell Line by Flow Cytometry. View Larger

Detection of CDO in C2C12 Mouse Cell Line by Flow Cytometry.

C2C12 mouse myoblast cell line was stained with Sheep Anti-Human CDO Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4384, filled histogram) or control antibody (Catalog # 5‑001‑A, open histogram), followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL010).

Immunocytochemistry CDO in  C2C12 Mouse Cell Line. View Larger

CDO in C2C12 Mouse Cell Line. CDO was detected in immersion fixed C2C12 mouse myoblast cell line using Sheep Anti-Human CDO Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4384) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cell membranes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CDO

CDO (CAM-related/down‑regulated by oncogenes, also CDON; pronounced “kid-oh”) is a 190 kDa member of the Immunoglubulin (Ig) superfamily, Ig/Fibronectin (FN) type III repeat family of cell surface proteins (1). Human CDO is a type I transmembrane (TM) glycoprotein. It is synthesized as a 1287 amino acid (aa) precursor that contains a 25 aa signal sequence, a 938 aa extracellular domain (ECD), a 21 aa TM segment and a 303 aa cytoplasmic region (1, 2). The ECD contains five C2‑type Ig-like domains, followed by three FN type III repeats. The first FN repeat (aa 577‑673) is known to bind numerous cadherins, while the third (or juxtramembrane) FN type III repeat (aa 826‑923) binds SHH (3, 4). The intracellular region is believed to signal through various bHLH transcription factors (2). One alternate splice form is reported that shows a deletion of aa 1212‑1234 in the cytoplasmic tail. The ECD of human CDO is 85% aa identical to mouse CDO ECD. CDO is found on muscle precursor and neural progenitor cells of the embryo (5, 6). It likely promotes muscle differentiation, and contributes to axon guidance and neuronal patterning (2, 7, 8, 9). These effects may be mediated through two different receptor complexes. On muscle precursors, CDO apparently acts as both a coordinating and signaling subunit. Here, it integrates N- and M-cadherin, neogenin, netrin-3 and BOC into a cis-oriented receptor complex (2). While this complex has no identified ligand, intercellular cadherin interactions or netrin, may be enough to trigger CDO/cadherin/neogenin signaling. On axons, CDO may participate in a poorly‑defined receptor complex minimally composed of CDO, BOC and Gas1 that binds SHH, and interacts with PTCH1 (7, 8, 10).

References
  1. Kang, J.S. et al. (1997) J. Cell Biol. 138:203. 
  2. Krauss, R.S. et al. (2005) J. Cell Sci. 118:2355. 
  3. Yao, S. et al. (2006) Cell 125:343. 
  4. Kang, J-S. et al. (2003) Proc. Natl. Acad. Sci. USA 100:3989. 
  5. Kang, J-S. et al. (2002) EMBO J. 21:114. 
  6. Zhang, W. et al. (2006) Mol. Cell. Biol. 26:3764.
  7. Okada, A. et al. (2006) Nature 444:369.
  8. Allen, B.L. et al. (2007) Genes Dev. 21:1244.
  9. Kang, J-S. et al. (2004) J. Cell. Biol. 167:493.
  10. Tenzen, T. et al. (2006) Dev. Cell 10:647.
Long Name
Cell Adhesion Molecule-related/Down-regulated by Oncogenes
Entrez Gene IDs
50937 (Human); 57810 (Mouse)
Alternate Names
CDO; Cdon homolog (mouse); CDON; CDOORCAM; cell adhesion molecule-related/down-regulated by oncogenes; MGC111524; ORCAM; surface glycoprotein, Ig superfamily member

Product Datasheets

Citation for Human CDO Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Cdon acts as a Hedgehog decoy receptor during proximal-distal patterning of the optic vesicle.
    Authors: Cardozo M, Sanchez-Arrones L, Sandonis A, Sanchez-Camacho C, Gestri G, Wilson S, Guerrero I, Bovolenta P
    Nat Commun, 2014;5(0):4272.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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