Human CEBP  alpha Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human CEBP  alpha in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) CEBP beta, rhCEBP gamma, rhCEBP δ, rhCEBP zeta, and rhCEBP epsilon is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human CEBP  alpha
    Met1-Ala124
    Accession # P49715
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
CEBP  alpha in Human Liver. CEBP  alpha was detected in immersion fixed paraffin-embedded sections of human liver using Sheep Anti-Human CEBP  alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7094) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CEBP alpha

CEBP alpha (CCAAT/Enhancer-Binding Protein; also C/EBP) is a 42-43 kDa member of the C/EBP subfamily, bZIP family of proteins. It is expressed in multiple cell types, particularly those that are fully differentiated, and includes hepatocytes, skeletal muscle cells, adipocytes, intestinal epithelium and proliferating macrophages. CEBP alpha acts as a transcriptional activator for a large and diverse number of genes, including glycogen synthase, GLUT4, and G-CSFR, and participates in both cell cycle progression and differentiation. Human CEBP alpha  is 358 amino acids (aa) in length). It contains one transactivation domain (aa 1-96), poly-Gly segment (aa 99-104), a poly-Pro motif (aa 183-189), a bZIP dimerization region (aa 281-334), and an overlapping DNA-binding Leu-zipper domain (aa 317-345). CEBP alpha  forms both homodimers, and heterodimers with other subfamily members. One alternative start site at Met120 generates a low activity 30-32 kDa isoform variant. There is also a utilized phosphorylation site at Ser21 that regulates activity. Over aa 1-124, human CEBP alpha shares 93% aa identity with mouse CEBP alpha.

  • Long Name:
    CCAAT/enhancer-Binding Protein alpha
  • Entrez Gene IDs:
    1050 (Human); 12606 (Mouse); 24252 (Rat)
  • Alternate Names:
    C/EBP alpha; C/EBP-alpha; CCAAT/enhancer binding protein (C/EBP), alpha; CCAAT/enhancer-binding protein alpha; CEBP alpha; CEBP; CEBPA
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