Protein kinases are the largest class of enzymes in the human genome. These enzymes regulate almost all cellular processes by adding phosphate groups to proteins, thereby modifying the activity, localization, and overall function of their targets. Consequently, abnormal activity of kinases underlies many diseases including cancer.
Proteome Profiler Human Cell Stress Array Kit
R&D Systems | Catalog # ARY018
Contains 4 membranes - each spotted in duplicate with 26 different cell stress antibodies
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Key Product Details
Species
Human
Product Summary for Proteome Profiler Human Cell Stress Array Kit
Kit Summary
A membrane-based antibody array for the parallel determination of the relative levels of selected human cell stress-related proteins. Validated for analyte detection in cell and tissue lysates.
Key Benefits
- Detects 26 human cell stress-related proteins simultaneously
- Requires no specialized equipment
Principle of the Array
The Proteome Profiler Human Cell Stress Array Kit is a membrane-based sandwich immunoassay. Samples are mixed with a cocktail of biotinylated detection antibodies (Step 1) and then incubated with the array membrane which is spotted in duplicate with capture antibodies to specific target proteins (Step 2). Captured proteins are visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of analyte bound.
Why Use an Antibody Array to Detect Cell Stress-Related Proteins?
Determining the expression of multiple cell stress-related proteins in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple proteins in a single sample can be cost-effective and also save time and sample.
Kit Contents
- 4 Array Membranes
- 4-Well Multi-dish
- Array Buffers
- Lysis Buffer
- Wash Buffer
- Detection Antibody Cocktail
- Streptavidin-HRP
- Chemiluminescent Detection Reagents
- Transparency Overlay Template
- Detailed Protocol
For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.
Stability and Storage
Store the unopened kit at 2 °C to 8 °C. Do not use past kit expiration date.
| Simultaneously detect the levels of these cell stress-related proteins in a single sample. | ||
|---|---|---|
| ADAMTS1 | HIF-2 alpha | Phospho-p38 alpha (T180/Y182) |
| Bcl-2 | Phospho-HSP27 (S78/S82) | Phospho-p53 (S46) |
| Carbonic Anhydrase IX | HSP60 | PON1 |
| Cited-2 | HSP70 | PON2 |
| COX-2 | IDO | PON3 |
| Cytochrome c | Phospho-JNK Pan (T183/Y185) | Thioredoxin-1 |
| Dkk-4 | NFkappaB1 | SIRT2 |
| FABP1/L-FABP | p21/CIP1 | SOD2 |
| HIF-1 alpha | p27/Kip1 | |
Assays for analytes represented in the Human Cell Stress Array Kit
| Analyte | Quantkine® ELISA Kits | DuoSet® ELISA Development Systems | DuoSet® IC ELISA Development Systems (Total) | DuoSet® IC ELISA Development Systems (Phospho) |
|---|---|---|---|---|
| ADAMTS1 | DY2197 | |||
| Bcl-2 | DYC827B | |||
| Carbonic Anhydrase IX | DCA900 | DY2188 | ||
| Cited-2 | ||||
| COX-2 | DYC4198 | |||
| Cytochrome c | DCTC0 | |||
| Dkk-4 | DY1269 | |||
| FABP1/L-FABP | ||||
| HIF-1 alpha | DYC1935 | |||
| HIF-2 alpha | ||||
| Phospho-HSP27 (S78/S82) | DYC1580 | DYC2314 | ||
| HSP60 | DYC1800 | |||
| HSP70 | DY1663 | DYC1663 | ||
| IDO | DY6030 | |||
| Phospho-JNK Pan (T183/Y185) | DYC1387B | DYC2236 | ||
| NFkappaB1 | ||||
| p21/CIP1 | DYC1047 | |||
| p27/Kip1 | DYC2256 | |||
| Phospho-p38 alpha (T180/Y182) | DYC8691B | DYC869B | ||
| Phospho-p53 (S46) | DYC1043 | |||
| PON1 | DYC5816 | |||
| PON2 | ||||
| PON3 | ||||
| Thioredoxin-1 | ||||
| SIRT2 | ||||
| SOD2 | DYC3419 |
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Scientific Data Images for Proteome Profiler Human Cell Stress Array Kit
Determination of Relative Levels of Selected Human Cell Stress-related Proteins.
HeLa human cervical epithelial carcinoma cells were either untreated or exposed to 150 J/m2 of UV light followed by a 30 minute recovery period before lysis.
Determination of Relative Levels of Selected Human Cell Stress-related Proteins.
The Human Cell Stress Array detects multiple analytes in tissue lysates. 300 µg of tissue lysate was run on each array. Data shown are from a 5 minute exposure to X-ray film.Formulation, Preparation, and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Cell Stress Array Kits
Additional Cell Stress Array Kits Products
Product Documents for Proteome Profiler Human Cell Stress Array Kit
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Proteome Profiler Human Cell Stress Array Kit
For research use only
Citations for Proteome Profiler Human Cell Stress Array Kit
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