Human Cell Stress Array


R&D Systems Human Cell Stress Array, Panel A (Catalog # ARY018) is a rapid, sensitive, and economical tool used to simultaneously detect the relative levels of 26 different cell stress-related proteins in a single sample. No specialized equipment is necessary.

Simultaneously detect the levels of these cell stress-related proteins in a single sample.
ADAMTS1 HIF-2 alpha Phospho-p38 alpha (T180/Y182)
Bcl-2 Phospho-HSP27 (S78/S82) Phospho-p53 (S46)
Carbonic Anhydrase IX HSP60 PON1
Cited-2 HSP70 PON2
Cytochrome c Phospho-JNK Pan (T183/Y185) Thioredoxin-1
Dkk-4 NFkappaB1 SIRT2
HIF-1 alpha p27/Kip1  

General Assay Principle

Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Cell or tissue lysate samples are diluted and mixed with a cocktail of biotinylated detection antibodies. The sample/antibody mixture is then incubated with the array. Any protein/detection antibody complex present is bound by its cognate immobilized capture antibody on the membrane. Streptavidin-Horseradish Peroxidase and chemiluminescent detection reagents are added, and a signal is produced in proportion to the amount of cytokine bound. Chemiluminescence is detected in the same manner as a Western blot.

Kit Contents

  • 4 Array Membranes
  • 4-Well Multi-dish
  • Array Buffers
  • Lysis Buffer
  • Wash Buffer
  • Detection Antibody Cocktail
  • Streptavidin-HRP
  • Chemiluminescent Detection Reagents
  • Transparency Overlay Template
  • Detailed Protocol

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.