R&D Systems Human Cell Stress Array, Panel A (Catalog # ARY018) is a rapid, sensitive, and economical tool used to simultaneously detect the relative levels of 26 different cell stress-related proteins in a single sample. No specialized equipment is necessary.
|Simultaneously detect the levels of these cell stress-related proteins in a single sample.
||Phospho-p38 alpha (T180/Y182)
|Carbonic Anhydrase IX
||Phospho-JNK Pan (T183/Y185)
General Assay Principle
Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Cell or tissue lysate samples are diluted and mixed with a cocktail of biotinylated detection antibodies. The sample/antibody mixture is then incubated with the array. Any protein/detection antibody complex present is bound by its cognate immobilized capture antibody on the membrane. Streptavidin-Horseradish Peroxidase and chemiluminescent detection reagents are added, and a signal is produced in proportion to the amount of cytokine bound. Chemiluminescence is detected in the same manner as a Western blot.
- 4 Array Membranes
- 4-Well Multi-dish
- Array Buffers
- Lysis Buffer
- Wash Buffer
- Detection Antibody Cocktail
- Chemiluminescent Detection Reagents
- Transparency Overlay Template
- Detailed Protocol
For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.