Human Total Bcl-2 DuoSet IC ELISA

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Product Details
Citations (9)
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Human Total Bcl-2 DuoSet IC ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
96-well strip plate
Assay Length
4 hours 40 mins (after plate preparation)
Sample Volume Required
Cell lysates (100 µL)
Assay Range
62.5 - 4,000 pg/mL
Sufficient Materials
Kits available for two, five, or fifteen 96-well plates*
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure total Bcl-2 in cell lysates. An immobilized capture antibody specific for Bcl-2 binds both phosphorylated and unphosphorylated Bcl-2. After washing away unbound material, a biotinylated detection antibody is used to detect both phosphorylated and unphosphorylated protein, utilizing a standard Streptavidin-HRP format.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Available in 2, 5, and 15- (96-well) plate pack sizes
  • Economical alternative to Western blot

Kit Content

  • Capture Antibody
  • Conjugated Detection Antibody
  • Calibrated Immunoassay Standard or Control
  • Streptavidin-HRP

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Lysis Buffer*

IC Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product

Data Example

Standard Curve

Product Datasheets

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Preparation and Storage

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Bcl-2

Bcl-2 is a member of a family of proteins that regulates outer mitochondrial membrane permeability. Bcl-2 is an anti-apoptotic member that prevents release of cytochrome c from the mitochondria intermembrane space into the cytosol. Bcl-2 is present on the outer mitochondrial membrane and is also found on other membranes in some cell types. Natural Bcl-2 contains a carboxyl-terminal mitochondria targeting sequence.

Long Name:
B Cell Lymphoma/Leukemia 2
Entrez Gene IDs:
596 (Human); 12043 (Mouse); 24224 (Rat)
Alternate Names:
apoptosis regulator Bcl-2; B-cell CLL/lymphoma 2; Bcl2; Bcl-2

Citations for Human Total Bcl-2 DuoSet IC ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Pancreatic secretory trypsin inhibitor reduces multi-organ injury caused by gut ischemia/reperfusion in mice
    Authors: RJ Playford, T Marchbank
    PLoS ONE, 2020;15(1):e0227059.
    Species: Human
    Sample Types: Cell Lysates
  2. The Effects of Silver Nanoparticles Biosynthesized Using Fig and Olive Extracts on Cutaneous leishmaniasis Induced Inflammation in Female Balb/c Mice
    Authors: MA Almayouf, MF El-Khadrag, MA Awad, EM Al-Olayan
    Bioscience Reports, 2020;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  3. MiR-507 inhibits the growth and invasion of trophoblasts by targeting CAMK4
    Authors: HQ Li, JJ Fan, XH Li, D Bao
    Eur Rev Med Pharmacol Sci, 2020;24(11):5856-5862.
    Species: Human
    Sample Types: Cell Lysates
  4. Probiotics and yogurt modulate oxidative stress and fibrosis in livers of Schistosoma mansoni-infected mice
    Authors: MF El-Khadrag, EM Al-Olayan, MIY Elmallah, AM Alharbi, HM Yehia, AE Abdel Mone
    BMC Complement Altern Med, 2019;19(1):3.
    Species: Mouse
    Sample Types: Tissue Homogenates
  5. Redox correlation in muscle lengthening and immune response in eccentric exercise
    Authors: F He, CC Chuang, T Zhou, Q Jiang, DA Sedlock, L Zuo
    PLoS ONE, 2018;13(12):e0208799.
    Species: Human
    Sample Types: Cell Lysates
  6. Oxidative stress-mediated apoptosis induced by ethanolic mango seed extract in cultured estrogen receptor positive breast cancer MCF-7 cells.
    Authors: Abdullah A, Mohammed A, Rasedee A, Mirghani M
    Int J Mol Sci, 2015;16(2):3528-36.
    Species: Human
    Sample Types: Cell Lysates
  7. Engagement of CD31 delivers an activating signal that contributes to the survival of chronic lymphocytic leukaemia cells.
    Authors: Poggi A, Prevosto C, Catellani S, Rocco I, Garuti A, Zocchi MR
    Br. J. Haematol., 2010;151(3):252-64.
    Species: Human
    Sample Types: Cell Lysates
  8. Bone morphogenetic proteins and receptors are over-expressed in bone-marrow cells of multiple myeloma patients and support myeloma cells by inducing ID genes.
    Authors: Grcevic D, Kusec R, Kovacic N, Lukic A, Lukic IK, Ivcevic S, Nemet D, Seiwerth RS, Ostojic SK, Croucher PI, Marusic A
    Leuk. Res., 2010;34(6):742-51.
    Species: Human
    Sample Types: Cell Lysates
  9. AMP-activated protein kinase-dependent and -independent mechanisms underlying in vitro antiglioma action of compound C.
    Authors: Vucicevic L, Misirkic M, Janjetovic K, Harhaji-Trajkovic L, Prica M, Stevanovic D, Isenovic E, Sudar E, Sumarac-Dumanovic M, Micic D, Trajkovic V
    Biochem. Pharmacol., 2009;77(11):1684-93.
    Species: Human
    Sample Types: Cell Lysates


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