Human HSP70/HSPA1A DuoSet ELISA

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    • Assay Type
      Solid Phase Sandwich ELISA
    • Format
      96-well strip plate
    • Sample Volume Required
      100 µL
    • Sufficient Materials
      For five 96-well plates*
    • Specificity
      Please see the product datasheet
    * Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
    Ancillary Reagent Kit Available
    DY008 , DuoSet ELISA Ancillary Reagent Kit 2 - Please Inquire
    This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human HSP70 / HSPA1A. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

    Product Features
    • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
    • Development protocols are provided to guide further assay optimization
    • Assay can be customized to your specific needs
    • Economical alternative to complete kits
    Kit Content
    • Capture Antibody
    • Detection Antibody
    • Recombinant Standard
    • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
    Other Reagents Required

    PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

    Wash Buffer: (Catalog # WA126), or equivalent

    Reagent Diluent*

    Blocking Buffer*

    Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

    Stop Solution: 2 N H2SO4 (Catalog # DY994)

    Microplates: R&D Systems (Catalog # DY990), or equivalent

    Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

    *For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

    Product Datasheets

    Certificate of Analysis Lookup

    To download a Certificate of Analysis, please enter a lot number in the search box below.

    Note: Certificate of Analysis not available for kit components.

    Certificate of Analysis Lookup

    Certificate of Analysis Request Form

    To download a Certificate of Analysis, please enter the catalog and lot numbers in the search box below.

    Note: Certificate of Analysis not available for kit components.

    Preparation and Storage
    • Stability & Storage
      Store the unopened product at 2 - 8 °C. Do not use past expiration date.
    Background: HSP70/HSPA1A
    The heat shock proteins are a highly conserved family of stress response proteins. HSPs function primarily as molecular chaperones, facilitating the folding of other cellular proteins, preventing protein aggregation, or targeting improperly folded proteins to specific degradative pathways. Some HSPs are expressed at low levels under normal physiological conditions but show dramatically increased expression in response to cellular stress, others are constitutively expressed. Specific HSPs play a role in regulating apoptosis by interacting directly with key components of the apoptotic pathway.
    • Long Name:
      Heat Shock Protein 70
    • Entrez Gene IDs:
      3303 (Human); 15511 (Mouse); 24472 (Rat)
    • Alternate Names:
      dnaK-type molecular chaperone HSP70-1; FLJ54303; FLJ54370; FLJ54392; FLJ54408; FLJ75127; Heat shock 70 kDa protein 1/2; heat shock 70 kDa protein 1A/1B; heat shock 70kD protein 1A; heat shock 70kDa protein 1A; heat shock-induced protein; HSP70; HSP70.1/HSP70.2; HSP70-1; HSP70-1/HSP70-2; HSP70-1A; HSP70I; HSP72; HSPA1; HSPA1A; HSPA1B

FAQs

No product specific FAQs exist for this product, however you may

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DuoSet Ancillary Reagent Kits
Description Application Cat# Citations Images  

DuoSet ELISA Ancillary Reagent Kit 2

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Supplemental ELISA Products
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Substrate Reagent Pack

ELISA DY999 59
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Reagent Diluent Concentrate 2

ELISA DY995 17
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Stop Solution 2N Sulfuric Acid

ELISA DY994 13
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ELISA Plate Sealers

ELISA DY992
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Streptavidin-HRP

ELISA DY998 46
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Quantikine ELISA Wash Buffer 1

ELISA WA126 6
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Clear Polystyrene Microplates

ELISA DY990 3
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Reagent Additive 1

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Glo Substrate Reagent Pack

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ELISA Plate-coating Buffer

ELISA DY006 1
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Reagent Diluent Concentrate 3 (5X)

ELISA DY004
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Black Polystyrene Microplates, 25 Pack

ELISA DY991 2
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Average Rating: 4 (Based on 1 review)

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 Human HSP70/HSPA1A DuoSet ELISA DY1663-05 Array DY1663-05
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Very Good
  Anonymous 04/18/2017
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 Human HSP70/HSPA1A DuoSet ELISA DY1663-05
: Human HSP70/HSPA1A DuoSet ELISA [DY1663-05].

Summary

Sample Tested EDTA Plasma

Other Experimental Details

Other Experimental Details We run human plasma samples at an 1:2 dilution for maximum precision. Samples could be run at a maximum 1:5 dilution with reduced precision, but decreased loss of sample volume,

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