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Human HSP70 / HSPA1A ELISA Standard Curve
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Citations (6)
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Human HSP70/HSPA1A DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
96-well strip plate
Sample Volume Required
100 µL
Assay Range
125.0 - 8,000 pg/mL
Sufficient Materials
For five 96-well plates*
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human HSP70 / HSPA1A. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data

Human HSP70 / HSPA1A ELISA Standard Curve

Product Datasheets

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Preparation and Storage

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: HSP70/HSPA1A

The heat shock proteins are a highly conserved family of stress response proteins. HSPs function primarily as molecular chaperones, facilitating the folding of other cellular proteins, preventing protein aggregation, or targeting improperly folded proteins to specific degradative pathways. Some HSPs are expressed at low levels under normal physiological conditions but show dramatically increased expression in response to cellular stress, others are constitutively expressed. Specific HSPs play a role in regulating apoptosis by interacting directly with key components of the apoptotic pathway.

Long Name:
Heat Shock Protein 70
Entrez Gene IDs:
3303 (Human); 15511 (Mouse); 24472 (Rat)
Alternate Names:
dnaK-type molecular chaperone HSP70-1; FLJ54303; FLJ54370; FLJ54392; FLJ54408; FLJ75127; Heat shock 70 kDa protein 1/2; heat shock 70 kDa protein 1A/1B; heat shock 70kD protein 1A; heat shock 70kDa protein 1A; heat shock-induced protein; HSP70; HSP70.1/HSP70.2; HSP70-1; HSP70-1/HSP70-2; HSP70-1A; HSP70I; HSP72; HSPA1; HSPA1A; HSPA1B

Citations for Human HSP70/HSPA1A DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Alterations of the 70�kDa heat shock protein (HSP70) and sequestosome-1 (p62) in women with breast cancer
    Authors: T Orfanelli, S Giannopoul, E Zografos, A Athanasiou, AM Bongiovann, G Doulaveris, TA Moo, D LaPolla, CN Bakoyianni, GE Theodoropo, GC Zografos, E Andreopoul, SS Witkin
    Scientific Reports, 2021-11-15;11(1):22220.
    Species: Human
    Sample Types: Cell Culture Supernates
  2. A tumor-intrinsic PD-L1-NLRP3 inflammasome signaling pathway drives resistance to anti-PD-1 immunotherapy
    Authors: B Theivanthi, KS Evans, NC DeVito, MP Plebanek, M Sturdivant, LP Wachsmuth, AK Salama, Y Kang, D Hsu, JM Balko, DB Johnson, M Starr, AB Nixon, A Holtzhause, BA Hanks
    J. Clin. Invest., 2020-05-01;0(0):.
    Species: Human
    Sample Types: Plasma
  3. Vaginal Biomarkers That Predict Cervical Length and Dominant Bacteria in the Vaginal Microbiomes of Pregnant Women
    Authors: SS Witkin, AF Moron, BJ Ridenhour, E Minis, A Hatanaka, SGP Sarmento, MS Franca, FHC Carvalho, TK Hamamoto, R Mattar, E Sabino, IM Linhares, MVC Rudge, LJ Forney
    MBio, 2019-10-22;10(5):.
    Species: Human
    Sample Types: Vaginal Fluid
  4. Intrapulmonary Autoantibodies to HSP72 Are Associated with Improved Outcomes in IPF
    Authors: R Mills, A Mathur, LM Nicol, JJ Walker, AA Przybylski, AC Mackinnon, SEM Howie, WAH Wallace, I Dransfield, N Hirani
    J Immunol Res, 2019-04-11;2019(0):1845128.
    Species: Human
    Sample Types: Serum
  5. Increased Plasma Levels of Danger-Associated Molecular Patterns Are Associated With Immune Suppression and Postoperative Infections in Patients Undergoing Cytoreductive Surgery and Hyperthermic Intraperitoneal Chemotherapy
    Authors: GP Leijte, H Custers, J Gerretsen, A Heijne, J Roth, T Vogl, GJ Scheffer, P Pickkers, M Kox
    Front Immunol, 2018-04-05;9(0):663.
    Species: Human
    Sample Types: Plasma
  6. Genotoxic stress modulates the release of exosomes from multiple myeloma cells capable of activating NK cell cytokine production: Role of HSP70/TLR2/NF-kB axis
    Authors: E Vulpis, F Cecere, R Molfetta, A Soriani, C Fionda, G Peruzzi, G Caracciolo, S Palchetti, L Masuelli, L Simonelli, U D'Oro, MP Abruzzese, MT Petrucci, MR Ricciardi, R Paolini, M Cippitelli, A Santoni, A Zingoni
    Oncoimmunology, 2017-01-13;6(3):e1279372.
    Species: Human
    Sample Types: Cell Lysates


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Reviews for Human HSP70/HSPA1A DuoSet ELISA

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By Jonatan Dereke on 04/18/2017
Sample Tested: EDTA Plasma

We run human plasma samples at an 1:2 dilution for maximum precision. Samples could be run at a maximum 1:5 dilution with reduced precision, but decreased loss of sample volume,