Human ADAMTS1 DuoSet ELISA

R&D Systems | Catalog # DY2197

R&D Systems
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Key Product Details

Assay Type

Solid Phase Sandwich ELISA

Assay Range

93.8-6000 pg/mL

Sample Type

Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet

Reactivity

Human

Human ADAMTS1 DuoSet ELISA Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits
View all ADAMTS1 ELISA Kits »

Product Summary for Human ADAMTS1 DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human ADAMTS1. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Specifications

Assay Format

96-well strip plate (sold separately)

Sample Volume Required

100 µL

Detection Method

Colorimetric ELISA - 450nm (TMB)

Conjugate

Biotin

Specificity

Please see the product datasheet

Label

HRP

Scientific Data Images for Human ADAMTS1 DuoSet ELISA

Human ADAMTS1 ELISA Standard Curve

Human ADAMTS1 ELISA Standard Curve

Kit Contents for Human ADAMTS1 DuoSet ELISA

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required


PBS:

(Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: ADAMTS1

Members of the ADAMTS family contain pro, catalytic, disintegrin-like, cysteine-rich, spacer and a variable number of TS type 1 motifs. In contrast to membrane-anchored ADAMs, ADAMTSs are secreted and are all active proteases. They are essential for aggrecan turnover and procollagen processing, regulation of which is important in many physiological and pathological processes such as angiogenesis and arthritis.

Long Name

A Disintegrin-like and Metalloproteinase Domain with Thrombospondin Motifs 1

Alternate Names

C3-C5, METH1

Entrez Gene IDs

9510 (Human)

Gene Symbol

ADAMTS1

Additional ADAMTS1 Products

Product Documents for Human ADAMTS1 DuoSet ELISA

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human ADAMTS1 DuoSet ELISA

For research use only

Related Research Areas

Metalloproteases and Regulators

Citations for Human ADAMTS1 DuoSet ELISA

Customer Reviews for Human ADAMTS1 DuoSet ELISA (2)

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  • Human ADAMTS1 DuoSet ELISA
    Name: Anonymous
    Sample Tested: Plasma and Urine
    Verified Customer | Posted 11/07/2022
    Detectable in low levels in plasma & urine at 2-fold. Standard curve was good.
    Human ADAMTS1 DuoSet ELISA DY2197
  • Human ADAMTS1 DuoSet ELISA
    Name: Anonymous
    Sample Tested: Serum and Plasma
    Verified Customer | Posted 09/23/2019
    This kit works well for detecting the ADAMTS 1 in human serum samples. Easy to use protocol and very good standard curve.
    Human ADAMTS1 DuoSet ELISA DY2197

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