Detects human Cerebellin-2 in direct ELISAs. In direct ELISAs, 100% cross-reactivity
with recombinant human (rh) Cerebellin-1 and rhCerebellin-4 is observed, and
approximately 50% cross-reactivity with rhCerebellin-3 is observed.
Monoclonal Mouse IgG1 Clone # 704323
Protein A or G purified from hybridoma culture supernatant
Chinese hamster ovary cell line CHO-derived recombinant human Cerebellin-2 Arg50-Leu224 Accession # Q8IUK8
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Cerebellin-2 in Human Brain. Cerebellin-2 was detected in immersion fixed paraffin-embedded sections of human brain (medulla) using Mouse Anti-Human Cerebellin-2 Monoclonal Antibody (Catalog # MAB70441) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Cerebellin-2 (CBLN2) is an approximately 25 kDa glycoprotein in the Cerebellin family of C1q- and TNF-related proteins. It contains a potential membrane-associating segment but may also be secreted. It contains one C1q-like domain in its extracellular region. Cerebellin-2 expression is developmentally regulated in the cerebellum, neocortex, sensory neurons of the dorsal root ganglion and spinal cord, and adrenal cortex. It associates with Cerebellin-1 which is involved in the formation and maintenance of synapses in the cerebellum. Within the ectodomain (aa 50-224), Human Cerebellin-2 shares 99% aa sequence identity with mouse and rat Cerebellin‑2.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.